A5018791841- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Sperm and Testicular Function
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Ovarian function and disorders
- Xenotransplantation and immune response
- Mesenchymal stem cell research
- Reproductive Physiology in Livestock
- Cancer Research and Treatments
- 3D Printing in Biomedical Research
- Veterinary Medicine and Surgery
- Genetic and phenotypic traits in livestock
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Additive Manufacturing and 3D Printing Technologies
- Sexual Differentiation and Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Electrospun Nanofibers in Biomedical Applications
- Muscle Physiology and Disorders
- Reproductive System and Pregnancy
Seoul National University
2016-2025
Airlangga University
2022-2023
Advanced Institute of Convergence Technology
2012-2021
New Generation University College
2018-2021
National Institute of Ecology
2019
Seoul National University Hospital
2017
Gyeonggi Research Institute
2015
Catholic University of Daegu
2012
Schön Klinik Neustadt
2004
Kobe University
2004
Melatonin is a multifunctional molecule that mediates several circadian and seasonal processes in animal reproduction. its metabolites are antioxidants free radical scavengers. We investigated the effects of melatonin on porcine oocyte maturation embryo development. then local expression receptor 1 (MT1) gene cumulus cells, granulosa oocytes with reverse transcription-polymerase chain reaction (RT-PCR) method. further evaluated antioxidant [reactive oxygen species (ROS) levels...
Mesenchymal stem cells (MSCs) are attractive candidates for clinical repair or regeneration of damaged tissues. Oct4 and Sox2, which essential transcription factors pluripotency self-renewal, naturally expressed in MSCs at low levels early passages, their gradually decrease as the passage number increases. Therefore, to improve MSC proliferation stemness, we introduced human Sox2 conferring higher expansion differentiation capabilities. The Oct4-IRES-Sox2 vector was transfected into adipose...
Over the world, canine species, including gray wolf, have been gradually endangered or extinct. Many efforts made to recover and conserve these canids. The aim of this study was produce wolf with somatic cell nuclear transfer (SCNT) for conservation. Adult ear fibroblasts from a female (Canis lupus) were isolated cultured in vitro as donor cells. Because limitations obtaining matured oocytes, vivo oocytes obtained by flushing oviducts isthmus infundibulum used. After removing cumulus cells,...
Livestock, particularly cattle, are crucial for biotechnology fields, such as genetic breeding, infectious diseases, bioreactors, and specific disease models. However, engineering in cattle has lagged due to long gestation periods, single embryo pregnancies, high rearing costs. Additionally, the slow validation of germline transmission absence germline-competent embryonic stem cells hinder progress. With development genome editing technologies like ZFN, TALEN, CRISPR-Cas9, recent...
Abstract Background As the global population increases, demand for protein sources is expected to increase, driving cell-based cultivated meat. This study aimed enhance productivity of meat through optimization cell source and organization process. Results We engineered fibroblasts into myogenic cells via non-viral introduction MYOD1 gene, avoiding viral methods safety. After confirming stable derivation cells, we combined knockout (KO) MSTN , a negative regulator myogenesis, with -mediated...
Abstract Cell penetrating peptides (CPPs) are that can be translocated into cells and used as a carrier platform for the intracellular uptake of cargo molecules. Subject to source CPP sequences their positively charged nature, cytotoxicity immunogenicity conventional CPPs needs optimized expand utility biomedical applications. In addition these safety issues, stability addressed since residues prone interact with biological milieu. As an effort overcome limitations current technology, we...
Genome editing has been considered as powerful tool in agricultural fields. However, genome progress cattle not fast other mammal species, for some disadvantages including long gestational periods, single pregnancy, and high raising cost. Furthermore, technically demanding methods such microinjection somatic cell nuclear transfer (SCNT) are needed gene cattle. In this point of view, electroporation embryos risen an alternative.First, efficiency our were tested embryos. Presence mutation on...
Other SectionsABSTRACTINTRODUCTIONINTRODUCTION OF GENE ENGINEERING TECHNOLOGIESIN VITRO ANIMAL PRODUCTION TECHNOLOGYAPPLICATION GENOME-ENGINEERED LIVESTOCK (FIG. 3)TRENDS IN FDA APPROVAL FOR GENETICALLY ENGINEERED LIVESTOCKDISCUSSIONACKNOWLEDGEMENTSCONFLICTS INTERESTFIGURESTABLEREFERENCES
Abstract Numerous studies have explored the cultivation of muscle cells using non-animal materials for cultivated meat production. Achieving cell proliferation and alignment 3D scaffolds made from plant-based remains challenging. This study introduces a technique to culture align only materials, avoiding toxic chemical modifications. Zein-alginate fibers (ZA fibers) were fabricated by coating zein protein onto alginate (A fibers). Zein’s excellent compatibility biodegradability enable high...
Abstract Dogs ( Canis familiaris ) share many common genetic diseases with humans and development of disease models using a transgenic approach has long been awaited. However, due to the technical difficulty in obtaining fertilizable eggs unavailability embryonic stem cells, no dog generated. Canine fetal fibroblasts were stably transfected red fluorescent protein RFP gene‐expressing construct retrovirus gene delivery method. Somatic cell nuclear transfer was then employed replace nucleus an...
Kisspeptin (Kp) is best known as a multifunctional peptide with roles in reproduction, the cardiovascular system and cancer. In present study expression of kisspeptin hierarchy elements (KISS1, GNRH1 LHB) their receptors (KISS1R, GNRHR LHCGR, respectively) porcine ovary cumulus-oocyte complexes (COCs) were investigated, its effects on vitro maturation (IVM) oocytes subsequent ability to sustain preimplantation embryo competence after parthenogenetic electrical activation. Kp expressed...
Quercetin is a plant-derived flavonoid found in fruits or vegetables that has antioxidant properties and acts as free radical scavenger.We investigated the effects of quercetin on porcine oocyte nuclear maturation embryonic development after parthenogenetic activation.We then evaluated activities by measuring reactive oxygen species (ROS) levels matured oocytes.Immature oocytes were untreated treated with 1, 10, 50 μg/mL during vitro (IVM).Quercetin treatment did not improve maturation, but...
Abstract Determining cell lineage and function is critical to understanding human physiology pathology. Although advances in tracing methods provide new insight into fate, defining cellular diversity at the mammalian level remains a challenge. Here, we develop genome editing strategy using cytidine deaminase fused with nickase Cas9 (nCas9) specifically target endogenous interspersed repeat regions cells. The resulting mutation patterns serve as genetic barcode, which induced by targeted...
Mesenchymal stem cells (MSCs) promote therapeutic angiogenesis to cure serious vascular disorders. However, their survival period and cytokine-secretory capacity are limited. Although hepatocyte growth factor (HGF) can accelerate the rate of angiogenesis, recombinant HGF is limited because its very short half-life (<3-5 minutes). Thus, continuous treatment with required obtain an effective response. To overcome these limitations, we produced genome-edited MSCs that secreted upon...
Many genome-edited animals have been produced using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology to edit specific genes. However, there are few guidelines for the application of this technique cattle. The goal study was produce trait-improved cattle genome-editing CRISPR-Cas9. Myostatin (MSTN) selected as a target locus, and synthetic mRNA sgRNA Cas9 were microinjected into fertilized bovine embryos in vitro. As result, 17 healthy calves born, three them...
Abstract Background There have been many efforts to develop efficient vaccines for the control of porcine reproductive and respiratory syndrome virus (PRRSV). Although inactivated PRRSV are preferred their safety, they weak at inducing humoral immune responses controlling field infection, especially when heterologous viruses involved. Results In all groups, sample positive (S/P) ratio IDEXX ELISA neutralization (VN) titer remained negative until challenge. While viremia did not reduce in...
Abstract Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) exhibit potency for the regeneration of infarcted hearts. Vascular endothelial growth factor (VEGF) is capable inducing angiogenesis and can boost cell-based therapeutic effects. However, high levels VEGF cause abnormal blood vessel hemangiomas. Thus, a controllable system to induce required cell therapy. We generated an inducible VEGF-secreting (VEGF/hUCB-MSC) that controls expression tested efficacy in rat...