A5100413516- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Animal Genetics and Reproduction
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Silk-based biomaterials and applications
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Invertebrate Immune Response Mechanisms
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- melanin and skin pigmentation
- DNA and Nucleic Acid Chemistry
- Genetics, Aging, and Longevity in Model Organisms
- Plant tissue culture and regeneration
- Iron-based superconductors research
- Studies on Chitinases and Chitosanases
- Electrospun Nanofibers in Biomedical Applications
- Genetic factors in colorectal cancer
- Skin Protection and Aging
- Supramolecular Self-Assembly in Materials
- Dental Implant Techniques and Outcomes
- Wnt/β-catenin signaling in development and cancer
Institute for Basic Science
2014-2024
Gachon University
2015-2024
Institute of Molecular Biotechnology
2018-2023
Austrian Academy of Sciences
2019-2023
Vienna Biocenter
2019-2023
Yonsei University
2008-2022
Jeonbuk National University
2007-2021
Gangnam Severance Hospital
2020
Gyeongsang National University
2020
Rural Development Administration
2020
Abstract We present a novel method of targeted gene disruption that involves direct injection recombinant Cas9 protein complexed with guide RNA into the gonad nematode Caenorhabditis elegans. Biallelic mutants were recovered among F1 progeny, demonstrating high efficiency this method.
The gastric corpus epithelium is the thickest part of gastrointestinal tract and rapidly turned over. Several markers have been proposed for stem cells in both isthmus base regions. However, identity (IsthSCs) interaction between distinct cell populations still under debate. Here, based on unbiased genetic labeling biophysical modeling, we show that glands are compartmentalized into two independent zones, with slow-cycling maintaining actively cycling pit-isthmus-neck region through a...
Abstract Nitric oxide (NO) is a crucial signaling molecule with various functions in physiological systems. Due to its potent biological effect, the preparation of responsive biomaterials upon NO having temporally transient properties challenging task. This study represents first therapeutic‐gas (i.e., NO)‐responsive hydrogel by incorporating NO‐cleavable crosslinker. The rapidly swollen response NO, and not other gases. Furthermore, NO‐responsive gel converted enzyme‐responsive gels cascade...
Adult stem cells constantly react to local changes ensure tissue homeostasis. In the main body of stomach, chief produce digestive enzymes; however, upon injury, they undergo rapid proliferation for prompt regeneration. Here, we identified p57Kip2 (p57) as a molecular switch reserve cell state in mice. During homeostasis, p57 is expressed but rapidly diminishes after followed by robust proliferation. Both single-cell RNA sequencing and dox-induced lineage tracing confirmed sequential loss...
Genome editing has been considered as powerful tool in agricultural fields. However, genome progress cattle not fast other mammal species, for some disadvantages including long gestational periods, single pregnancy, and high raising cost. Furthermore, technically demanding methods such microinjection somatic cell nuclear transfer (SCNT) are needed gene cattle. In this point of view, electroporation embryos risen an alternative.First, efficiency our were tested embryos. Presence mutation on...
Livestock, particularly cattle, are crucial for biotechnology fields, such as genetic breeding, infectious diseases, bioreactors, and specific disease models. However, engineering in cattle has lagged due to long gestation periods, single embryo pregnancies, high rearing costs. Additionally, the slow validation of germline transmission absence germline-competent embryonic stem cells hinder progress. With development genome editing technologies like ZFN, TALEN, CRISPR-Cas9, recent...
Seventeen saponins isolated from the root of Pulsatilla koreana were examined for their in vitro cytotoxic activity against human solid cancer cell lines, A-549, SK-OV-3, SK-MEL-2, and HCT15, using SRB assay method, vivo antitumor BDF1 mice bearing Lewis lung carcinoma (LLC). The 5-17, with a free acidic functional group at C-28 aglycon, exhibited moderate to considerable activity, however, 1-4, esterified trisaccharide did not exhibit (ED50; >300 microM). Among them, oleanolic acid...
A rhodamine triazole-based fluorescent chemosensor has been developed for the selective detection of platinum ions in aqueous solutions. The 6G hydroxamate linked with a propargyl group is converted to corresponding triazole by “click” reaction. dual binding unit composed and shows high selectivity sensitivity toward Pt2+ over range other metal water. probe applied monitor cisplatin
Emphysema is one of the characteristic features chronic obstructive pulmonary disease, which caused mainly by cigarette smoking. Recent data have suggested that apoptosis and cell cycle arrest may contribute to development emphysema. In this study, we addressed question whether how smoke affected Akt, plays a critical role in survival proliferation. normal human lung fibroblasts, extract (CSE) death, accompanying degradation total phosphorylated Akt (p-Akt), was inhibited MG132. CSE exposure...
Due to the morphological resemblance between electrospun nanofibers and extracellular matrix (ECM), fibers have been widely used fabricate scaffolds for tissue regeneration. Relationships scaffold morphologies cells are cell type dependent. In this study, we sought determine an optimum fiber diameter human vascular smooth muscle (VSMC) regeneration in scaffolds. Scaffolds were produced using poly(caprolactone) (PCL) diameters of 0.5, 0.7, 1, 2, 2.5, 5, 7 or 10 μm, VSMC survivals,...
Novel photocatalysts (TiO<sub>2</sub>@TiO<sub>1−x</sub>N<sub>x</sub>) with the core–shell structure, prepared by controlled nitridation of TiO<sub>2</sub> microspheres, show photocatalytic activity under visible-light irradiation.
Indolocarbazole-pyridine hybrid foldamers are strongly fluorescent in an extended random conformation, but the fluorescence is completely quenched upon folding to a helical conformation due compact stacking between aryl planes backbone. Anion binding disturbs thus regenerating of foldamers. This unique property has been utilized develop turn-on probe for anions such as sulfate and fluoride.
The hydroacylation of methanol with alkenes was developed using a catalytic system consisting Rh(I), 2-amino-4-picoline and benzoic acid; the reaction is speculated to occur by initial N-methylation methanol, subsequent dehydrogenation resulting N-methylamine, followed double chelation-assisted hydroimination alkene imine give dialkyl ketones after hydrolysis.
Modification of proteins at serine or threonine residues with N-acetylglucosamine, termed O-GlcNAcylation, plays an important role in most eukaryotic cells. To understand the molecular mechanism by which O-GlcNAcylation regulates entry Caenorhabditis elegans into non-aging dauer state, we performed proteomic studies using two mutant strains: O-GlcNAc transferase-deficient ogt-1(ok430) strain and O-GlcNAcase-defective oga-1(ok1207) strain. In presence pheromone daumone, ogt-1 showed...
Abstract A considerable number of studies have focused on the regulation mothers against decapentaplegic homologue (Smad)–dependent or –independent pathways in signaling by each transforming growth factor β (TGF-β) superfamily member diverse biologic contexts. The sophisticated actions these molecules and underlying molecular mechanisms still remain elusive. Here we show new ambilateral R (receptor-regulated)–Smad bone morphogenetic protein 2 (BMP-2)/TGF-β1 signals. In a specific context,...
Abstract The study aimed to investigate the effects of silk fibroin in a mouse model dry eye. experimental eye was developed using more than twelve-weeks-old NOD.B10. H2 b mice exposing them 30–40% ambient humidity and injecting with scopolamine hydrobromide for 10 days. Tear production corneal irregularity score were measured by instillation phosphate buffered saline or fibroin. Corneal detachment conjunctival goblet cell density observed hematoxylin eosin periodic acid Schiff staining...
Abstract Glycyl‐tRNA synthetase 1 (GARS1), a cytosolic enzyme secreted from macrophages, promotes apoptosis in cancer cells. However, the mechanism underlying GARS1 secretion has not been elucidated. Here, we report that is through unique extracellular vesicles (EVs) with hydrodynamic diameter of 20–58 nm (mean diameter: 36.9 nm) and buoyant density 1.13–1.17 g/ml. was anchored to surface these EVs palmitoylated C390 residue. Proteomic analysis identified 164 proteins were uniquely enriched...
Rho‐family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in closure of the Drosophila larval epidermis. Pak3‐deficient larvae failed to close hole this defect was rescued Pak1 expression, indicating differential functions two proteins. Pak3 localized margin, which selectively required Rac1. showed severe defects actin‐myosin organization at margin...