A5103160672- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Microbial infections and disease research
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Respiratory viral infections research
- Veterinary medicine and infectious diseases
- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- Coccidia and coccidiosis research
- Viral gastroenteritis research and epidemiology
- Mycotoxins in Agriculture and Food
- Reproductive tract infections research
- Aquaculture disease management and microbiota
- Reproductive System and Pregnancy
- Herpesvirus Infections and Treatments
- Chromosomal and Genetic Variations
- Reproductive Physiology in Livestock
- Tissue Engineering and Regenerative Medicine
- Milk Quality and Mastitis in Dairy Cows
- Mesenchymal stem cell research
- Transgenic Plants and Applications
- Endometriosis Research and Treatment
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
Seoul National University
2005-2025
Genome editing has been considered as powerful tool in agricultural fields. However, genome progress cattle not fast other mammal species, for some disadvantages including long gestational periods, single pregnancy, and high raising cost. Furthermore, technically demanding methods such microinjection somatic cell nuclear transfer (SCNT) are needed gene cattle. In this point of view, electroporation embryos risen an alternative.First, efficiency our were tested embryos. Presence mutation on...
Other SectionsABSTRACTINTRODUCTIONINTRODUCTION OF GENE ENGINEERING TECHNOLOGIESIN VITRO ANIMAL PRODUCTION TECHNOLOGYAPPLICATION GENOME-ENGINEERED LIVESTOCK (FIG. 3)TRENDS IN FDA APPROVAL FOR GENETICALLY ENGINEERED LIVESTOCKDISCUSSIONACKNOWLEDGEMENTSCONFLICTS INTERESTFIGURESTABLEREFERENCES
Understanding the molecular characteristics and metabolic processes of mammalian endometrium is crucial for advancing biological research, particularly in veterinary obstetrics pathology. This study established analyzed organoids from endometrial epithelial stem cells five mammals with different placental types: cows (cotyledonary), dogs cats (zonary), pigs (diffuse), rats (discoid). The these species were maintained over 13 passages, successfully frozen-thawed, confirmed by pathological...
Livestock, particularly cattle, are crucial for biotechnology fields, such as genetic breeding, infectious diseases, bioreactors, and specific disease models. However, engineering in cattle has lagged due to long gestation periods, single embryo pregnancies, high rearing costs. Additionally, the slow validation of germline transmission absence germline-competent embryonic stem cells hinder progress. With development genome editing technologies like ZFN, TALEN, CRISPR-Cas9, recent...
The chronologic localization of Mycoplasma hyopneumoniae was examined by in situ hybridization experimentally infected pigs for a period 35 days after intratracheal inoculation. M. DNA detected bronchial and bronchiolar epithelial cells from at 7, 14, 21, 28 postinoculation (DPI) alveolar interstitial macrophages type I pneumocytes 28, DPI. Strong signals were mainly the luminal surface lining cells. When signal cells, given bronchus or bronchiole also exhibited peribronchiolar lymphoid...
Mycoplasma hyopneumoniae DNA was detected in 20 naturally infected pigs by situ hybridization using a nonradioactive digoxigenin-labeled probe. A 520-base-pair probe targeting reiterative sequence of the M. genome generated polymerase chain reaction. All with had distinct and positive signals without background staining. strong signal mainly luminal surface bronchial bronchiolar lining epithelial cells, whereas no seen cytoplasm cells. When given bronchus or bronchiole peribronchiolar...
The detection and distribution of interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α) IL-6 were studied, by in situ hybridization with a non-radioactive digoxigenin-labeled probe, formalin-fixed, paraffin-embedded lung tissue from 10 pigs naturally infected Mycoplasma hyopneumoniae. morphology host cells was preserved despite the relatively high temperature required during incubation procedure. Examination three serial sections each samples showed that cytokines closely resembled other...
Abstract Transgene silencing provides a significant challenge in animal model production via gene engineering using viral vectors or transposons. Selecting an appropriate strategy, contingent upon the species is crucial to circumvent transgene silencing, necessitating long-term observation of vivo expression. This study employed PiggyBac transposon create GFP rat address rats. Surprisingly, occurred while CAG promoter, contrary conventional understanding, whereas Ef1α promoter prevented...
Gnotobiotic piglets were inoculated intralaryngeally with swine Chlamydia trachomatis strain R33 or orally C. trachmatis R27. Archived formalin-fixed, paraffin-embedded tissues from euthanatized 4-7 days postinoculation examined by in situ hybridization for nucleic acid using a nonradioactive digoxigenin-labeled DNA probes that targeted specific ribosomal RNA ompl mRNA molecules of the strains. Positive signals detected bronchial epithelial cells, bronchiolar pneumocytes, alveolar and...
In situ hybridization techniques that employed a nonradioactive digoxigenin-labeled probe were used to detect and localize ApxI, II III genes in tissue sections of pneumonic lung naturally infected with Actinobacillus pleuropneumoniae. pigs either serotype 2 or 6, signal for apxIICA, apxIIICA, apxIBD, apxIIIBD was detected, 5, apxICA, apxIBD detected the lesions. A apxIICA 7. strong apx seen streaming degenerate alveolar leukocytes bordering zones coagulative necrosis. Simultaneous detection...
Transgenic pigs are promising animal resources for human disease models and organ donors xenotransplantation, because they resemble humans anatomically physiologically. have been produced from transfected donor cells using several gene delivery systems including retrovirus infection. Recently, it has reported that piggyBac (PB) transposition is a highly efficient tool in producing transgenic mice. This study investigated the use of PB to establish produce cloned embryos pigs. We constructed...
Abstract As the understandings about biotechnology and pathophysiology of diseases getting advanced, genetic materials or genetically engineered cells have brought hopes on patients with inherited diseases. Among many congenital diseases, muscular dystrophy has been globally one major subjects therap. To apply therapy selectively in tissue, promoters which express genes specifically muscle necessitated by researchers. In current study, promoter region MSTN gene was postulated as candidate...
<title>Abstract</title> Background Livestock, particularly cattle, are crucial for biotechnology fields, such as genetic breeding, infectious diseases, bioreactors, and specific disease models. However, engineering in cattle has lagged due to long gestation periods, single embryo pregnancies, high rearing costs. Additionally, the slow validation of germline transmission absence germline-competent embryonic stem cells hinder progress. With development genome editing technologies like ZFN,...
Gene therapy is a promising method for treating inherited diseases by directly delivering the correct genetic material into patient cells. However, limited packaging capacity of vectors poses challenge. Minimizing promoter size viable strategy among various approaches to address this issue. This study aims optimize bovine myostatin (MSTN) promoter, enhancing its utility in gene applications. We identified primary driver activity as proximal regulatory region. Isolated from native and termed...
Several DNA transposons including PiggyBac (PB), Sleeping Beauty (SB), and Tol2 have been applied as effective means for of transgenesis in many species. Cattle are not typically experimental animals, relatively little verification has presented on this Thus, the goal here was to determine applicability three transposon systems somatic embryo cells cattle, while also investigating which is appropriate each cell type. Green fluorescent protein (GFP)-expressing were used electroporation...
Abstract Background: Several DNA transposons, PiggyBac (PB), Sleeping beauty (SB) and Tol2 have been applied as effective means for transgenesis in many species. Cattle are not typical experimental animals, relatively little verification has studied this Thus, the goal of study was applicability three transposon systems somatic embryo cells cattle, while also determining which is appropriate each type cell. To conduct experiment, green fluorescent protein (GFP)-expressing were used...