A5100403290- Alzheimer's disease research and treatments
- Force Microscopy Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Electrochemical sensors and biosensors
- Nanocluster Synthesis and Applications
- thermodynamics and calorimetric analyses
- Glaucoma and retinal disorders
- Molecular Junctions and Nanostructures
- Atmospheric Ozone and Climate
- Corneal surgery and disorders
- Mechanical and Optical Resonators
- Electrochemical Analysis and Applications
- Conducting polymers and applications
- Thermography and Photoacoustic Techniques
- Ionosphere and magnetosphere dynamics
- Membrane Separation and Gas Transport
- Advanced Fluorescence Microscopy Techniques
- CRISPR and Genetic Engineering
- Plant Surface Properties and Treatments
- Prion Diseases and Protein Misfolding
- Cellular Mechanics and Interactions
- Carbon and Quantum Dots Applications
- Optical Coherence Tomography Applications
Yonsei University
2010-2023
Korea National University of Transportation
2021-2022
Korea University
2019-2021
Severance Hospital
2017
It is of high importance to measure and map the surface charge distribution amyloids, since electrostatic interaction between amyloidogenic proteins biomolecules plays a vital role in amyloidogenesis. In this work, we have measured mapped distributions amyloids (i.e., β-lactoglobulin fibril) using Kelvin probe force microscopy. shown that highly dependent on conformation (e.g., helical pitch amyloid fibrils) as well pH solvent.
In recent years, Kelvin probe force microscopy (KPFM) has emerged as a versatile toolkit for exploring electrical properties on broad range of nanobiomaterials and molecules. An analysis using KPFM can provide valuable sample information including surface potential work function certain material. Accordingly, been widely used in the areas material science, electronics, biomedical science. this review, we will briefly explain setup its measuring principle then survey representative results...
Abstract Purpose To assess the associations between vascular and metabolic comorbidities prevalence of open‐angle glaucoma ( OAG ) with low‐teen high‐teen intraocular pressure IOP in Korea. Methods Cross‐sectional data from Korean National Health Nutrition Examination Survey 2008 to 2012 were analysed. Participants diagnosed normal further classified into ≤ 15 mmHg) (15 mmHg < 21 groups. Using multiple logistic regression analyses, investigated for low‐ Results The prevalences...
The detection of circulating protein (CP) is very important for the diagnosis and therapeutics cancer. Conventional techniques based on a specific antibody–antigen interaction are still lacking because shortage cost effectiveness, complicated sandwich structure tagging process, inconsistent CP due to inherent instability antibodies. Herein, we demonstrate hybrid device consisting two-dimensional (2D) nanoscale molybdenum disulfide (MoS2) field-effect transistor (FET) with an amyloid-β1–42...
Graphene-based e-textile gas sensors have received significant attention as wearable electronic devices for human healthcare and environmental monitoring. Theoretically, more the attached graphene on devices, better is gas-sensing performance. However, it has been hampered by poor adhesion between textile platforms. Meanwhile, amyloid nanofibrils are reputed their ability to improve materials, including microorganisms. Despite that fact, there no attempt apply fabricate graphene-based...
Abstract Amyloid fibrils are a hallmark of neurodegenerative diseases and exhibit conformational diversity that governs their pathological functions. Despite recent findings concerning the role diversity, way in which heterogeneous conformations amyloid can be formed has remained elusive. Here, we show microwave-assisted chemistry affects self-assembly process fibril formation, results heterogeneity. In particular, allows for delicate control thermodynamics process, enabled us to tune...
Abstract The generation of toxic amyloid β (Aβ) oligomers is a central feature the onset and progression Alzheimer’s disease (AD). Drug discoveries for Aβ oligomer degradation have been hampered by difficulty purification lack screening tools. Here, we report plasmonic nanoparticle corona (PNAC) quantifying efficacy oligomeric aggregate-degrading drugs. Our strategy to monitor drug-induced aggregates analyzing colorimetric responses PNACs. To test our strategy, use Aβ-degrading proteases...
To investigate the change of anterior chamber angle morphology and intraocular pressure (IOP) reduction after cataract surgery in patients with normal-tension glaucoma (NTG) using swept-source optical coherence tomography (SS-OCT).This prospective, comparative, observational study recruited into two groups. Group 1 was control group including normal subjects except those cataracts (cataract group, n=67 eyes 67 patients), 2 NTG who were diagnosed (n=43 43 which treated phacoemulsification...
Determination of the conformation (monomer, oligomer, or fibril) amyloid peptide aggregates in human brain is essential for diagnosis and treatment Alzheimer’s disease (AD). Accordingly, systematic investigation using analytical tools precisely quantifying relative amounts three conformations peptide. Here, we developed a reduced graphene oxide (rGO) based multiplexing biosensor that could be used to monitor various amyloid-β 40 (Aβ40) fluids. The electrical rGO was composed multichannel...
Kelvin probe force microscopy (KPFM) is a robust toolkit for profiling the surface potential (SP) of biomolecular interactions between DNAs and/or proteins at single molecule level. However, it has often suffered from background noise and low throughput due to instrumental or environmental constraints, which regarded as limiting KPFM applications detection minute changes in molecular structures such single-nucleotide polymorphism (SNP). Here, we show imaging DNA-capped nanoparticles (DCNP)...
Amyloid-β (Aβ) peptides can be aggregated into β-sheet rich fibrils or plaques and deposited on the extracellular matrix of brain tissues, which is a hallmark Alzheimer's disease. Several drug candidates have been designed to retard progression neurodegenerative disorder eliminate toxic Aβ aggregates. Recently, 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid (EPPS) has emerged as promising for elimination However, effect EPPS degradation aggregates such not yet fully elucidated. In this...
Here, we demonstrate a powerful method to discriminate DNA mismatches at single-nucleotide resolution from 0 5 (χ0 χ5) using Kelvin probe force microscopy (KPFM). Using our previously developed method, quantified the surface potentials (SPs) of individual DNA-capped nanoparticles (DCNPs, ∼100 nm). On each DCNP, hybridization occurs between ∼2200 immobilized (pDNA) and target with (tDNA, ∼80 nM). Thus, DCNP used in bioassay (each pDNA-tDNA interaction) corresponds single ensemble which large...
Melanoma is visible unlike other types of cancer, but it still challenging to diagnose correctly because the difficulty in distinguishing between benign nevus and melanoma. We conducted a robust investigation melanoma, identifying considerable differences local elastic properties melanoma tissues by using atomic force microscopy (AFM) indentation histological specimens. Specifically, histograms modulus displayed multimodal Gaussian distributions, exhibiting heterogeneous mechanical...
Amyloid aggregates have emerged as a significant hallmark of neurodegenerative diseases such Alzheimer's and Parkinson's diseases. Although it has been recently reported that microwave heating induces amyloid aggregation compared with conventional methods, the mechanism aggregate induction remained unclear. In this study, we investigated formation oligomeric (OAAs) by irradiation at microscale volumes solution. Microwave protein monomer solution triggered rapid OAAs within 7 min. We...
The interplay between metal ions and amyloids plays a significant role not only in the pathogenesis of neurodegenerative disorders such as Alzheimer's Parkinson's disease but also development application technologies for synthesis nanomaterials. In this study, we investigate heavy using screen-printed electrochemical sensor functionalized with amyloid fibrils. For purpose, fibril-functionalized was devised. Various (e.g., palladium, mercury, copper, cadmium) directly reacted fibrils at...
Amyloid fibrils have recently been highlighted due to their excellent mechanical properties, which not only play a role in biological functions but also imply applications biomimetic material design. Despite recent efforts unveil how the properties of amyloid originate, it has remained elusive anisotropic nanomechanical hierarchically structured are determined. Here, we characterize using atomic force microscopy experiments and atomistic simulations. It is shown that hierarchical structure...