A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, universal delivery immortalized primary mammalian using surface-modified vertical silicon nanowires. The method relies on the ability nanowires to penetrate cell’s membrane subsequently release surface-bound molecules directly cytosol, thus allowing highly...

Zeolites are crystalline nanoporous aluminosilicates widely used in industry. In order for zeolites to find applications as innovative materials, they need be organized into large two- and three-dimensional (2D 3D) arrays. We report that uniformly aligned polyurethane films can serve templates the synthesis of 2D possibly 3D arrays silicalite-1 crystals, which orientations crystals controlled by nature polymers. propose supramolecularly organic-inorganic composites consist hydrolyzed organic...

Atoms, molecules, enzymes, proteins, DNAs, RNAs, nanoparticles, microparticles, tiles, and bricks are some of the most commonly encountered building blocks in chemistry architecture. These can be grouped into subnanometer (atoms), nanometer (molecules, nanoparticles), micrometer (microparticles), millimeter-to-centimeter (tiles bricks) based on their sizes. One important applications is to organize them as monolayers various substrates. Selfassembly has been method choice for monolayer...

Interactions between cells and the extracellular matrix (ECM) alter cellular behaviors, including adhesion, migration, proliferation, differentiation via focal adhesions that link ECM to actin cytoskeleton as an...

Intracellular thermometry is a powerful method for studying biological thermodynamics across various physiological contexts. In this study, we present an organelle-specific quantum utilizing nitrogen-vacancy (NV) centers in fluorescent nanodiamonds (FNDs) to obtain precise temperature measurements at the subcellular level. By conjugating antibodies, FNDs were selectively targeted mitochondria, nuclei, and cell membranes living fibroblasts, enabling real-time monitoring of changes during...

Which way to turn: The tendency of microcrystals attach surfaces through their largest faces has resulted in two orientations zeolite L monolayers on glass. Fluorophore-containing cylindrical and hexagonal columnar crystals assemble into vertically horizontally oriented monolayers, respectively, glass plates (see pictures). photoluminescences the are highly anisotropic.

Graphene domains with different sizes and densities were successfully grown on Cu foils use of a chemical vapor deposition method. We investigated the effects volume ratios argon to hydrogen during annealing process graphene growth, especially as function partial pressure. The mean size density increased an increase in pressure time. In addition, we found that only gas resulted snowflake-shaped carbon aggregates. Energy-dispersive X-ray spectroscopy (EDX) high-resolution photoemission...

Investigation of molecular interactions in polymeric films is crucial for understanding and engineering multiscale physical phenomena correlated to device function performance, but this often involves a compromise between theoretical experimental data, because poor film uniformity. Here, we report the intramolecular intermolecular inside ultrathin conformal hybrid organic-inorganic alucone grown by layer deposition, based on sequential self-limiting surface reactions. Varying carbon chain...

In this work, we report that high-density, vertically grown silicon nanowires (vg-SiNWs) direct a new in vitro developmental pathway of primary hippocampal neurons. Neurons on vg-SiNWs formed single, extremely elongated major neurite earlier than minor neurites, which led to accelerated polarization. Additionally, the development lamellipodia, generally occurs 2D culture coverslips, was absent vg-SiNWs. The results indicate surface topography is an important factor influences neuronal and...

Isocyanate groups readily form urethane linkages with surface hydroxy on glass and zeolites this phenomenon was utilized in the assembly of monolayers zeolite microcrystals by employing diisocyanates as novel molecular binders.

Development of methods enabling the preparation uniformly aligned polymer thin films at molecular level is a prerequisite for realizing their optoelectronic characteristics as innovative materials; however, these often involve compromise between scalability and accuracy. In this study, we have grown polyurea on SiO2 substrate using layer deposition (MLD) based sequential self-limiting surface reactions. By integrating plane-polarized Fourier-transform infrared, Raman spectroscopic tools,...

Cellular adhesion and growth on substrates with differing surface topography are known to induce unusual cell behaviors. Here, we investigated the of human embryonic kidney 293T cells vertically aligned silicon nanowires. Varying diameter nanowires affected their elasticity, which in turn caused variations morphology adhesion. Calculation elastic modulus revealed that long thin easier deflect thus provide more focal sites for adherent cells. And, induced mechanical tension triggered cellular...

We studied the differences in antibiotic susceptibilities of microorganisms that causeing urinary tract infections (UTI) children to obtain useful information on appropriate drug selection for childhood UTI.We retrospectively analyzed 429 isolated from 900 patients diagnosed with UTI Department Pediatrics, Chungbuk National University Hospital, 2003 2008.The most common causative were Escherichia coli (81.4%), Klebsiella pneumoniae (8.4%), Enterobacter spp. (1.7%), and Proteus (0.4%). E....

Do-it-yourself: Monoschichten von Molekülen und Nanopartikeln sollten auf den Substraten nur durch Selbstorganisation erzeugt werden. Für Mikropartikeln kommt als Alternative das direkte Aufbringen Hand hinzu, bei dem sich die Partikel wie Fliesen einpassen (siehe Bild). Die Qualität der händisch erzeugten übertrifft diejenige Produkte aus Selbstorganisationsverfahren. Supporting information for this article is available on the WWW under...

Here, we investigated the translocation of biomolecules, such as DNA and protein, through a sequentially polymerized polyurea nanopore, with thin (<10 nm) polymer membrane uniform thickness.

Abstract Anisotropic graphene domains are of significant interest since the electronic properties pristine strongly depend on its size, shape, and edge structures. In this work, considering that growth is governable by dynamics graphene-substrate interface during growth, we investigated shape defects grown copper lattices with different indices chemical vapor deposition methane at either low pressure or atmospheric pressure. Computational modeling identified crystallographic orientation...

Biosilification is of interest due to its capability produce a highly intricate structure under environmentally friendly conditions. Despite the considerable effort that has been devoted toward biomimetic silification, synthesis complex silica structures, as found in structures diatom cell walls, still infancy. Here, we report bioinspired fabrication well-organized and symmetric nanostructured networks, involving phase separation silicic acid polymerization processes, analogy morphogenesis...

Graphene has attracted great attention owing to its unique structural and electrical properties. Among various synthetic approaches of the graphene, metal assisted chemical vapor deposition (CVD) is most reasonable proper method produce large-scale low-defect graphene films. Until now, CVD from gaseous hydrocarbon sources shown promises for growth, but high growth temperature required such growth. A recent work by using liquid benzene precursor that monolayer could be obtained at 300 degrees...

Nanotopological cues are popular tools for in vivo investigation of the extracellular matrix (ECM) and cellular microenvironments. The ECM is composed multiple components generates a complex microenvironment. development accurate methods important disease diagnosis therapy, as well studies on cell behavior. Here, we fabricated anodized aluminum oxide (AAO) membranes using sulfuric oxalic acid under controlled voltage temperature. were designed to possess three different pore interpore sizes,...