A5071754158- Viral Infectious Diseases and Gene Expression in Insects
- Anesthesia and Neurotoxicity Research
- Neonatal Respiratory Health Research
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Allergic Rhinitis and Sensitization
- Ubiquitin and proteasome pathways
- Infant Development and Preterm Care
- Glycosylation and Glycoproteins Research
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Birth, Development, and Health
- CAR-T cell therapy research
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Genetic Mapping and Diversity in Plants and Animals
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- Mitochondrial Function and Pathology
- Chromosomal and Genetic Variations
- interferon and immune responses
- Essential Oils and Antimicrobial Activity
- Advanced Proteomics Techniques and Applications
- Meat and Animal Product Quality
- Plant Genetic and Mutation Studies
Duke-NUS Medical School
2019-2025
Bioprocessing Technology Institute
2015-2025
Agency for Science, Technology and Research
2015-2025
Singapore Institute of Technology
2023-2025
National University of Singapore
2003-2024
Japan Science and Technology Agency
2024
Weatherford College
2023
Northeast Agricultural University
2018-2020
A*STAR Graduate Academy
2018
Singapore Bioimaging Consortium
2009-2015
Linking the heavy chain (HC) and light (LC) genes required for monoclonal antibodies (mAb) production on a single cassette using 2A peptides allows control of LC HC ratio reduces non-expressing cells. Four derived from foot-and-mouth disease virus (F2A), equine rhinitis A (E2A), porcine teschovirus-1 (P2A) Thosea asigna (T2A), respectively, were compared expression 3 biosimilar IgG1 mAbs in Chinese hamster ovary (CHO) cell lines. linked by different both absence presence GSG linkers....
Translocation of a nascent protein from the cytosol into ER mediated by its signal peptide is critical step in secretion. The aim this work was to develop platform technology optimize peptides for high level production therapeutic antibodies CHO cells. A database large number human immunoglobulin (Ig) heavy chain (HC) and kappa light (LC) generated. Most HC contain 19 amino acids which can be divided three domains LC 22 acids. were then clustered according sequence similarity. Based on...
The present study sought to use microbial transglutaminase (MTG) modulate the physicochemical properties of brown rice protein isolate (BRPI), pea (PPI) and their blend, determine MTG's effect on structural digestive profile changes. MTG at concentration 1 U/g significantly increased water holding capacity (91.6% enhancement) decreased oil (39.3% reduction) BRPI + PPI (4:6) respectively, accompanied by decreasing solubility zeta potential values in aqueous system. From rheological analysis,...
Colorectal cancer is the second leading killer worldwide and presently most common among males in Singapore. The study aimed to detect changes of protein profiles associated with process colorectal tumorigenesis identify specific markers for early detection diagnosis or as potential therapeutic targets. Seven pairs tissues adjacent normal mucosa were examined by two-dimensional gel electrophoresis at basic pH range (pH 7–10). Intensity 34 spots detected statistical significance. 16...
To cite this article: Kidon MI, Chin CW, Kang LW, Ching OT, Seng TY, Ning WK, Angus AC, Theng OS, Feng GY, Reginald K, Zhi BX, Shen SH & Tim CF. Mite component–specific IgE repertoire and phenotypes of allergic disease in childhood: The tropical perspective. Pediatr Allergy Immunol 2011; 22 : 202–210. Sensitization to perennial aeroallergens correlates with the risk persistent asthma (AS) children. In Singapore, multiple codominant species mites abound indoor environment, preferential...
The innate immune system senses cytosolic dsDNA and bacterial cyclic dinucleotides initiates signaling via the adaptor STING to induce type 1 interferon (IFN) response. We demonstrate here that BTK-deficient cells have impaired IFN-β production TBK1/IRF3 activation when stimulated with agonists or infected pathogens activate signaling. BTK interacts DDX41 helicase. kinase SH3/SH2 interaction domains of bind, respectively, DEAD-box domain transmembrane region STING. phosphorylates DDX41, its...
Akt2 and its downstream effectors mediate insulin-stimulated GLUT4-storage vesicle (GSV) translocation fusion with the plasma membrane (PM). Using mass spectrometry, we identify actin-capping protein Tropomodulin 3 (Tmod3) as an Akt2-interacting partner in 3T3-L1 adipocytes. We demonstrate that Tmod3 is phosphorylated at Ser71 on activation, phosphorylation required for GLUT4 PM insertion glucose uptake. Phosphorylated regulates insulin-induced actin remodelling, essential step GSV PM....
Olive oil polyphenol extract (OOPE) has been reported to have antibacterial activity; however, its effect on Listeria monocytogenes is less studied so far. This study, thus, aimed reveal antimicrobial activity and action approach against L. via evaluating the minimum inhibitory concentration (MIC) as well changes of intracellular adenosine 5'-triphosphate (ATP) concentration, cell membrane potential, bacterial protein, DNA, morphology. The results showed that OOPE could inhibit growth with a...
House dust mites (HDM) such as Dermatophagoides pteronyssinus and farinae represent a major cause of type 1 allergies worldwide. Hence large quantities well-characterized HDM extracts are needed to prepare pharmaceutical-grade allergy vaccines. To this aim, the present study was undertaken define optimal conditions for large-scale cultures.D. D. were grown on different media combining various proportions wheat germ, yeast synthetic amino acids (the latter resembling composition human stratum...
Fluorescent small molecules have become indispensable tools for biomedical research along with the rapidly developing optical imaging technology. We report here a neural stem cell specific boron-dipyrromethane (BODIPY) derivative compound of designation red 3 (CDr3), developed through high throughput/content screening in-house generated diversity oriented fluorescence library in cells at different developmental stages. This novel specifically detects living both human and mouse origin....
The cell-by date: fluorescent compound CDy1 selectively stains embryonic stem cells (see scheme). was used to identify fibroblasts undergoing reprogramming induced pluripotent prior the expression of green protein under control Oct4 promoter. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please note: publisher is responsible for content...
Seipin regulates lipid homeostasis by preventing droplet (LD) formation in non-adipocytes but promoting it developing adipocytes. Here, we report that seipin interacts with 14-3-3β through its N- and C-termini. Expression of is upregulated during adipogenesis, deletion results defective adipogenesis without affecting key adipogenic transcription factors. We further identified the actin-severing protein cofilin-1 as an interacting partner to 14-3-3β. Cofilin-1 was spatiotemporally recruited...
Phosphopeptides play a crucial role in many biological processes and constitute some of the most powerful biomarkers disease detection. However they are often present very low concentration, which makes their detection highly challenging. Here, we demonstrate use solution-dispersible graphene-titania platform for selective extraction phosphopeptides from peptide mixtures. This is followed by direct analysis surface-enhanced laser desorption/ionization time-of-flight mass spectrometry...
The repression of telomerase activity during cellular differentiation promotes replicative aging and functions as a physiological barrier for tumorigenesis in long-lived mammals, including humans. However, the underlying mechanisms remain largely unclear. Here we describe how miR-615-3p represses hTERT expression. mir-615-3p is located an intron HOXC5 gene, member highly conserved homeobox family transcription factors controlling embryogenesis development. Unexpectedly, found that HoxC5 also...
Abstract Chinese hamster ovary (CHO) cells are the most prevalent mammalian cell factories for producing recombinant therapeutic proteins due to their ability synthesize human‐like post‐translational modifications and ease of maintenance in suspension cultures. Currently, a wide variety CHO host lines has been developed; substantial differences exist phenotypes even when transfected with same target vector. However, relatively less is known about influence inherited genetic heterogeneity on...
Amaranthus tricolor has been reported to contain some antimicrobial compounds, such as alkaloids, polyphenols, and terpenoids. However, its effect on Staphylococcus aureus less well researched. Therefore, this study was designed evaluate the activity possible mechanism of action crude extract (ATCE) against S. potential application in cooked meat. The assessed by disk diffusion, minimum inhibitory concentration (MIC) determinations, growth curve. changes bacterial membrane potential,...
A comprehensive characterization of biotherapeutics, mandated by regulatory authorities, requires analyses a protein drug at multiple structure levels. Such multilevel can be performed mass spectrometry (MS), with established conventional MS-based assays product quality attributes (PQAs) comprising intact and subunit middle-up MS analytes resolved on C4 column, bottom-up peptide mapping C18 column. Recent advances in have facilitated the increasing use middle-down analysis, expanding...
During muscle differentiation, mitochondria undergo dramatic changes in their morphology and distribution to prepare for the higher rate of energy consumption. By applying a mitochondria-targeted rosamine library C2C12 myogenesis, we discovered one compound that controls differentiation. When treated undifferentiated myoblasts, our selected compound, B25, inhibited myotube formation, when fully differentiated myotubes, it induced fission multinucleated myotubes into mononucleated fragments....
A set of mutated Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) elements with varying strengths is generated by mutating the translation initiation codons 10th, 11th, and 12th AUG to non-AUG triplets. They are able control relative expression multiple genes over a wide range in mammalian cells both transient stable transfections. The strength each IRES mutant remains similar different cell lines not gene specific. expressed proteins have correct molecular weights....