A5035682350- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Cancer therapeutics and mechanisms
- Cancer-related gene regulation
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- Digestive system and related health
- Renal and related cancers
- Pluripotent Stem Cells Research
- Advanced biosensing and bioanalysis techniques
Institute of Molecular and Cell Biology
2020-2023
Agency for Science, Technology and Research
2020-2023
Massachusetts General Hospital
2017
Harvard University
2017
Polycomb group gene silencing Histone proteins wrap around DNA to form nucleosomes that package metazoan into the nucleus. Chromatin compaction is also believed be critical for repression of homeotic genes by repressive complex 1 (PRC1) during development. Lau et al. used mutagenesis and expression analyses examine this mechanism. Altering PRC1 region affected transcriptional patterning mouse body axis. Thus, chromatin may drive stable heritable involved in patterning. Science , issue p. 1081
Polycomb group (PcG) proteins maintain the repressed state of lineage-inappropriate genes and are therefore essential for embryonic development adult tissue homeostasis. One critical function PcG complexes is modulating chromatin structure. Canonical repressive complex 1 (cPRC1), particularly its component CBX2, can compact phase-separate in vitro. These activities hypothesized to be forming a physical environment cells. While much has been learned by studying these cell culture models, it...
Regulation of global transcription output is important for normal development and disease, but little known about the mechanisms involved. DNA topoisomerase I (TOP1) an enzyme well-known its role in relieving supercoils enabling transcription. Here, we report a non-enzymatic function TOP1 that downregulates RNA synthesis. This dependent on specific DNA-interacting residues located conserved protein surface. A loss-of-function knock-in mutation this surface, R548Q, sufficient to cause...
Summary Polycomb group (PcG) proteins maintain the repressed state of lineage-inappropriate genes and are therefore essential for embryonic development adult tissue homeostasis. One critical function PcG complexes is modulating chromatin structure. Canonical repressive complex 1 (cPRC1), particularly its component CBX2, can compact phase separate in vitro . These activities hypothesized to be forming a physical environment cells. While much has been learned by studying these cell culture...