Steve Rozen
A5078961996- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Gastrointestinal Tumor Research and Treatment
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Head and Neck Cancer Studies
- Lymphoma Diagnosis and Treatment
- Genomics and Rare Diseases
- Gastrointestinal disorders and treatments
- Lung Cancer Treatments and Mutations
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Chromatin Remodeling and Cancer
- Nephrotoxicity and Medicinal Plants
- Scientific Computing and Data Management
- Evolution and Genetic Dynamics
- Cervical Cancer and HPV Research
- Gastric Cancer Management and Outcomes
Duke-NUS Medical School
2015-2024
National Cancer Centre Singapore
2012-2024
University of Virginia
2024
Duke University
2024
SingHealth
2017-2023
National Heart Centre Singapore
2017-2023
National University of Singapore
2012-2022
Howard Hughes Medical Institute
1998-2015
Duke Medical Center
2009-2015
Duke University Hospital
2009-2015
Designing PCR and sequencing primers are essential activities for molecular biologists around the world. This chapter assumes acquaintance with principles practice of PCR, as outlined in, example, refs. 1–4.
Polymerase chain reaction (PCR) is a basic molecular biology technique with multiplicity of uses, including deoxyribonucleic acid cloning and sequencing, functional analysis genes, diagnosis diseases, genotyping discovery genetic variants. Reliable primer design crucial for successful PCR, over decade, the open-source Primer3 software has been widely used design, often in high-throughput genomics applications. It also incorporated into numerous publicly available packages web services....
Choosing appropriate primers is probably the single most important factor affecting polymerase chain reaction (PCR). Specific amplification of intended target requires that do not have matches to other targets in certain orientations and within distances allow undesired amplification. The process designing specific typically involves two stages. First, flanking regions interest are generated either manually or using software tools; then they searched against an nucleotide sequence database...
Abstract Somatic mutations in cancer genomes are caused by multiple mutational processes, each of which generates a characteristic signature 1 . Here, as part the Pan-Cancer Analysis Whole Genomes (PCAWG) Consortium 2 International Cancer Genome (ICGC) and The Atlas (TCGA), we characterized signatures using 84,729,690 somatic from 4,645 whole-genome 19,184 exome sequences that encompass most types cancer. We identified 49 single-base-substitution, 11 doublet-base-substitution, 4...
Single-nucleotide polymorphisms (SNPs) are the most frequent type of variation in human genome, and they provide powerful tools for a variety medical genetic studies. In large-scale survey SNPs, 2.3 megabases genomic DNA was examined by combination gel-based sequencing high-density variation-detection chips. A total 3241 candidate SNPs were identified. map constructed showing location 2227 these SNPs. Prototype genotyping chips developed that allow simultaneous 500 The results...
Abstract Cholangiocarcinoma (CCA) is a hepatobiliary malignancy exhibiting high incidence in countries with endemic liver-fluke infection. We analyzed 489 CCAs from 10 countries, combining whole-genome (71 cases), targeted/exome, copy-number, gene expression, and DNA methylation information. Integrative clustering defined 4 CCA clusters—fluke-positive (clusters 1/2) are enriched ERBB2 amplifications TP53 mutations; conversely, fluke-negative 3/4) exhibit copy-number alterations PD-1/PD-L2 or...
A physical map has been constructed of the human genome containing 15,086 sequence-tagged sites (STSs), with an average spacing 199 kilobases. The project involved assembly a radiation hybrid 6193 loci and incorporated genetic linkage 5264 loci. This information was combined results STS-content screening 10,850 against yeast artificial chromosome library to produce integrated map, anchored by maps. provides coverage 99 percent 94 genome. also represents early step in international generate...
The human genome is thought to harbor 50,000 100,000 genes, of which about half have been sampled date in the form expressed sequence tags. An international consortium was organized develop and map gene-based tagged site markers on a set two radiation hybrid panels yeast artificial chromosome library. More than 16,000 genes mapped relative framework that contains 1000 polymorphic genetic markers. gene unifies existing physical maps with nucleotide protein databases fashion should speed...
A map of 30,181 human gene–based markers was assembled and integrated with the current genetic by radiation hybrid mapping. The new gene contains nearly twice as many genes previous release, includes most that encode proteins known function, is twofold to threefold more accurate than version. redesigned, informative functional World Wide Web site ( www.ncbi.nlm.nih.gov/genemap ) provides mapping information associated data annotations. This resource constitutes an important infrastructure...
Gastric cancer is a major gastrointestinal malignancy for which targeted therapies are emerging as treatment options. This study sought to identify the most prevalent molecular targets in gastric and elucidate systematic patterns of exclusivity co-occurrence among these targets, through comprehensive genomic analysis large panel cancers.Using high-resolution single nucleotide polymorphism arrays, copy number alterations were profiled 233 cancers (193 primary tumours, 40 cell lines) 98...
Background The development of plasma biomarkers could facilitate early detection, risk assessment and therapeutic monitoring in Alzheimer's disease (AD). Alterations ceramides sphingomyelins have been postulated to play a role amyloidogensis inflammatory stress related neuronal apoptosis; however few studies conducted comprehensive analysis the sphingolipidome AD using analytical platforms with accuracy, sensitivity reproducibility. Methods Findings We prospectively analyzed from 26 patients...