Charles Auffray
A5055008823- Bioinformatics and Genomic Networks
- Asthma and respiratory diseases
- Genetics, Bioinformatics, and Biomedical Research
- T-cell and B-cell Immunology
- Gene expression and cancer classification
- Immune Cell Function and Interaction
- Gene Regulatory Network Analysis
- Monoclonal and Polyclonal Antibodies Research
- IL-33, ST2, and ILC Pathways
- Microbial Metabolic Engineering and Bioproduction
- Biomedical Text Mining and Ontologies
- Immunotherapy and Immune Responses
- Molecular Biology Techniques and Applications
- Glycosylation and Glycoproteins Research
- Health, Environment, Cognitive Aging
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Nutrition, Genetics, and Disease
- RNA and protein synthesis mechanisms
- Genomic variations and chromosomal abnormalities
- Genomics and Chromatin Dynamics
- Cell Adhesion Molecules Research
- Biomedical and Engineering Education
- Computational Drug Discovery Methods
- RNA Research and Splicing
- Respiratory and Cough-Related Research
Centre National de la Recherche Scientifique
2015-2024
Université Claude Bernard Lyon 1
2015-2024
European Institute for Systems Biology and Medicine
2015-2024
Inserm
2011-2024
Karolinska Institutet
2020-2023
Karolinska University Hospital
2023
Centre International de Recherche en Infectiologie
2016-2021
Lung Institute
2021
Imperial College London
2017-2021
Institute for Systems Biology
2011-2020
A procedure is described for the large-scale purification of light (L) and heavy (H) chain mRNAs from plasmacytomas produced in mice. Intact RNA selectively precipitated high yield frozen tumors homogenized 3 M LiCl 6 urea. L H-chain were purified by oligo(dT)-cellulose chromatography either sucrose gradient centrifugation conditions preventing aggregation or means high-resolution preparative gel electrophoresis under non-denaturing conditions. γ2a α sedimented as major components at 15.5 S...
The human genome is thought to harbor 50,000 100,000 genes, of which about half have been sampled date in the form expressed sequence tags. An international consortium was organized develop and map gene-based tagged site markers on a set two radiation hybrid panels yeast artificial chromosome library. More than 16,000 genes mapped relative framework that contains 1000 polymorphic genetic markers. gene unifies existing physical maps with nucleotide protein databases fashion should speed...
A map of 30,181 human gene–based markers was assembled and integrated with the current genetic by radiation hybrid mapping. The new gene contains nearly twice as many genes previous release, includes most that encode proteins known function, is twofold to threefold more accurate than version. redesigned, informative functional World Wide Web site ( www.ncbi.nlm.nih.gov/genemap ) provides mapping information associated data annotations. This resource constitutes an important infrastructure...
U-BIOPRED is a European Union consortium of 20 academic institutions, 11 pharmaceutical companies and six patient organisations with the objective improving understanding asthma disease mechanisms using systems biology approach. This cross-sectional assessment adults severe asthma, mild/moderate healthy controls from countries consisted analyses patient-reported outcomes, lung function, blood airway inflammatory measurements. Patients (nonsmokers, n=311; smokers/ex-smokers, n=110) had more...
High-throughput technologies for DNA sequencing and analyses of transcriptomes, proteomes metabolomes have provided the foundations deciphering structure, variation function human genome relating them to health disease states. The increased efficiency opens up possibility analyzing a large number individual genomes complete reference are within reach using powerful analytical techniques based on chromatography, mass spectrometry nuclear magnetic resonance. Computational mathematical tools...
The lymphocyte activation gene 3 (LAG-3), expressed in human activated T and natural killer (NK) cells, is closely related to CD4 at the protein levels. We report here initial characterization of LAG-3-encoded protein. have generated two monoclonal antibodies after immunization mice with a 30-amino acid peptide that corresponds an exposed extra loop region present LAG-3 immunoglobulin-like first domain. reactivity these reagents directed against since they recognize both membrane-expressed...
Asthma is characterised by heterogeneous clinical phenotypes. Our objective was to determine molecular phenotypes of asthma analysing sputum cell transcriptomics from 104 moderate-to-severe asthmatic subjects and 16 nonasthmatic subjects. After filtering on the differentially expressed genes between eosinophil- noneosinophil-associated inflammation, we used unbiased hierarchical clustering 508 gene set variation analysis specific sets. We defined three transcriptome-associated clusters...
Medicine and healthcare are undergoing profound changes. Whole-genome sequencing high-resolution imaging technologies key drivers of this rapid crucial transformation. Technological innovation combined with automation miniaturization has triggered an explosion in data production that will soon reach exabyte proportions. How we going to deal exponential increase production? The potential "big data" for improving health is enormous but, at the same time, face a wide range challenges overcome...
We propose an innovative, integrated, cost-effective health system to combat major non-communicable diseases (NCDs), including cardiovascular, chronic respiratory, metabolic, rheumatologic and neurologic disorders cancers, which together are the predominant problem of 21st century. This proposed holistic strategy involves comprehensive patient-centered integrated care multi-scale, multi-modal multi-level systems approaches tackle NCDs as a common group diseases. Rather than studying each...
Abstract Personalized medicine is a term for revolution in that envisions the individual patient as central focus of healthcare future. The “personalized medicine”, however, fails to reflect enormous dimensionality this new will be predictive, preventive, personalized, and participatory‐a vision we have termed P4 medicine. This reflects paradigm change how practiced revolutionary rather than evolutionary. arises from confluence systems approach digitalization creates large data sets...
Asthma is a heterogeneous disease driven by diverse immunologic and inflammatory mechanisms.Using transcriptomic profiling of airway tissues, we sought to define the molecular phenotypes severe asthma.The transcriptome derived from bronchial biopsies epithelial brushings 107 subjects with moderate asthma were annotated gene set variation analysis using 42 signatures relevant asthma, inflammation, immune function. Topological data clinical histologic was performed derive clusters, nearest...
BackgroundAlthough several studies link high levels of IL-6 and soluble receptor (sIL-6R) to asthma severity decreased lung function, the role trans-signaling (IL-6TS) in asthmatic patients is unclear.ObjectiveWe sought explore association between epithelial IL-6TS pathway activation molecular clinical phenotypes patients.MethodsAn gene signature obtained from air-liquid interface cultures human bronchial cells stimulated with sIL-6R was used stratify transcriptomic data (Unbiased Biomarkers...
Researchers around the world join forces to reconstruct molecular processes of virus-host interactions aiming combat cause ongoing pandemic.
A set of 1091 human skeletal muscle cDNA clone inserts representing more than 800 gene transcripts were spotted as PCR products at high density on nylon membranes. Replicas the filters hybridized in stringent conditions with 33P-radiolabeled probes transcribed from poly(A)+ RNA. Hybridization signals collected phosphor screens and processed using a software specifically adapted for this application to identify quantitate each spot. Parameters likely influence hybridization signal intensity...