A5019858433- Angiogenesis and VEGF in Cancer
- Plant Virus Research Studies
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Nematode management and characterization studies
- Insect-Plant Interactions and Control
- Plant Disease Management Techniques
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Erythrocyte Function and Pathophysiology
- Plant Parasitism and Resistance
- Transcranial Magnetic Stimulation Studies
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Bone Tissue Engineering Materials
- Numerical methods in engineering
- Neuroscience and Neural Engineering
- Developmental Biology and Gene Regulation
- Advanced ceramic materials synthesis
- Cancer, Hypoxia, and Metabolism
- Geotechnical Engineering and Underground Structures
- Electrospun Nanofibers in Biomedical Applications
- Legume Nitrogen Fixing Symbiosis
- Blood properties and coagulation
- Ubiquitin and proteasome pathways
Henan Cancer Hospital
2021-2024
Zhengzhou University
2021-2024
University of Michigan–Dearborn
2022-2024
Rensselaer Polytechnic Institute
2018-2023
Wilmington University
2023
Beihang University
2010-2022
University at Albany, State University of New York
2022
Ocean University of China
2017-2022
Huashan Hospital
2021
Fudan University
2021
The human genome is thought to harbor 50,000 100,000 genes, of which about half have been sampled date in the form expressed sequence tags. An international consortium was organized develop and map gene-based tagged site markers on a set two radiation hybrid panels yeast artificial chromosome library. More than 16,000 genes mapped relative framework that contains 1000 polymorphic genetic markers. gene unifies existing physical maps with nucleotide protein databases fashion should speed...
We evaluated homologous recombination deficient (HRD) phenotypes in epithelial ovarian cancer (EOC) considering BRCA1, BRCA2 and RAD51C a large well-annotated patient set. EOC patients for germline deleterious mutations (n = 899), somatic 279) epigenetic alterations 482) these genes using NGS genome-wide methylation arrays. Deleterious were identified 32 (3.6%) 28 (3.1%) 26 (2.9%) RAD51C. Ten somatically sequenced had alterations, six (2.1%) BRCA1 four (1.4%) BRCA2. Fifty two (10.8%)...
Significance The handedness of cells, also known as cell chirality, has become widely accepted with the determination chirality using micropatterning techniques. While reliable, this system only works cells that can attach, subsequently polarize, and migrate on a 2D protein-coated substrate, which does not necessarily recapitulate 3D cellular environment. With unique biomaterial-based approach, we now determine less adhesive and/or polarized in setting. Thus, it becomes much easier for...
The vertebrate body plan is overall symmetrical but left-right (LR) asymmetric in the shape and positioning of internal organs. Although several theories have been proposed, biophysical mechanisms underlying LR asymmetry are still unclear, especially role cell chirality, at cellular level, on organ asymmetry. Here with developing chicken embryos, we examine whether intrinsic chirality or handedness regulates cardiac C looping. Using a recently established biomaterial-based 3D culture...
Abstract Objective S1P was found to protect the ESG by inhibiting MMP activity‐dependent shedding of in cultured endothelial cell studies. We aimed further test that contributes maintenance normal vascular permeability protecting intact microvessels. Methods quantified post‐capillary venules rat mesentery and measured albumin presence absence 1 μ M S1P. also inhibitors compared with those predicted a transport model for inter‐endothelial cleft. Results S1P, fluorescence intensity FITC...
The handedness of the cell regulates permeability blood vessels.
To develop a mechanical model with which to investigate the relationship between crimping morphology of collagen fibrils and nonlinear behavior cornea.Uniaxial tensile experiments were performed corneal strips test their behavior. A constitutive was constructed based on Gaussian-distributed crimped fibrils. The parameters that represent micro characteristics determined by fitting experimental data model. Transmission electron microscopy (TEM) used visualize in stroma. quantitative analysis...
Abstract VEGF-loaded core/shell fibrous membranes were prepared by coaxial electrospinning with dextran (DEX) as the core component and poly(lactide-co-glycolide) (PLGA) shell polymer, respectively. The electrospun DEX/PLGA fibers observed scanning electron microscopy, transmission microscopy confocal to identify fiber structure protein distribution. results of tensile tests showed that possessed lower strength higher Young's modulus than PLGA one. release profiles demonstrated vascular...
SUMMARY The aim of this study is to investigate the dynamic response a multi‐segment model thoracolumbar spine and determine how sitting posture affects under impact ejection. A nonlinear finite element thoracolumbar‐pelvis complex (T9–S1) was developed validated. multi‐body pilot also constructed so an ejection seat restraint system could be incorporated into model. distribution trunk mass on each vertebra considered in Dynamics analysis showed that induced obvious axial compression...
Seven new ophiobolins (1-5, 12, and 14) along with the 11 known analogues (6-11, 13, 15-18) were isolated from ethyl acetate extracts of liquid solid cultures mangrove fungus Aspergillus ustus 094102. The structures including absolute configurations seven compounds elucidated by spectroscopic analysis, chemical methods, quantum ECD calculations. Compounds 4-8 11-15 showed cytotoxicities against G3K, MCF-7, MD-MBA-231, MCF/Adr, A549, HL-60 human cancer cell lines IC50 values ranging 0.6 to 9.5 μM.
The disruption of endothelial heparan sulfate (HS) is an early event in tumor cell metastasis across vascular barriers, and the reinforcement HS reduces adhesion to endothelium. Our recent study showed that while growth factor (VEGF) greatly at vitro blood–brain barrier (BBB) formed by human cerebral microvascular cells (hCMECs), it significantly enhances on a breast cancer cell, MDA-MB-231 (MB231). Here, we tested this differential effect VEGF favors MB231 transmigration. We also if agents...
To investigate the effect of tumor cell adhesion on microvascular permeability (P) in intact microvessels, we measured rate human mammary carcinoma MDA-MB-231, hydraulic conductivity (L(p)), P, and reflection coefficient (σ) to albumin microvessels at initial after ∼45 min perfusion postcapillary venules rat mesentery vivo. Rats (Sprague-Dawley, 250-300 g) were anesthetized with pentobarbital sodium given subcutaneously. A midline incision was made abdominal wall, gently taken out arranged...
In order to play different roles in vascular functions as a mechanosensor blood flows and barrier transvascular exchange, the endothelial surface glycocalyx (ESG) should have an organized structure. Due limitations of optical electron microscopy, ultra-structure ESG has not been revealed until recent development super-resolution STORM.To investigate components their organization on bEnd3 (mouse brain microvascular cells) monolayer.ESG was immunolabeled with anti-heparan sulfate (HS),...
Although a human eye comprises less than 0.1% of the frontal body surface area, injuries to are found be disproportionally common in survivors explosions. This study aimed introduce Lagrangian–Eulerian coupling model predict globe rupture resulting from primary blast effect. A finite element was created using Lagrangian mesh. An explosive and its surrounding air domain were modelled Eulerian Coupling two models allowed simulating wave generation, propagation interaction with eye. The results...
To investigate whether vascular endothelial growth factor (VEGF) enhances cancer cell adhesion to normal microvessels, we used in vivo video microscopy measure rates of MDA-MB-435s human breast cells and ErbB2-transformed mouse mammary carcinomas the postcapillary venules rat mesentery. An individual venule mesentery was injected via a glass micropipette with either perfusate mammalian Ringer solution containing 1% bovine serum albumin as control, or addition 1 nm VEGF for test measurements....
Pore size and porosity have been widely acknowledged as important structural factors in tissue-engineered scaffolds. In fact, scaffolds with similar pore can provide varied permeability due to different shape, interconnectivity tortuosity. However, the effects of scaffold on seeded cells remains largely unknown during tissue regeneration vitro. this study, we measured Darcy (K) tri-calcium phosphate by distributed them into three groups: Low, Medium High. As a result, cell proliferation,...