Twelve new compounds, variecolorins A–L (1–12), together with eleven known analogues (13–23) were isolated from the broth of a halotolerant fungus, Aspergillus variecolor. The structures compounds 1–12 determined by chemical and spectroscopic methods. Compounds 1–11, 13–15, 20–23 exhibited weak radical scavenging activity against DPPH, IC50 values 43 to 103 µM. all essentially nontoxic P388, HL-60, BEL-7402, A-549 cell lines 70 260

In order to search for structurally novel and bioactive natural compounds from microorganisms, a halotolerant fungal strain, Penicillium citrinum B-57, which mainly produces citrinin derivatives, was isolated sediments collected the Jilantai salt field. From ethyl acetate extract of P. two new dimers, pennicitrinone C (1) penicitrinol B (2), 11 known related were identified by spectroscopic chemical methods. These showed antioxidative activity against DPPH radicals with IC50 values ranging 0.8 59 µM.

Six new tetramic acids derivatives, penicillenols A1, A2, B1, B2, C1, and C2 (1—6), together with citrinin, phenol A acid, A, dihydrocitrinin, were identified from Penicillium sp. GQ-7, an endophytic fungus associated Aegiceras corniculatum. Their structures elucidated on the basis of comprehensive spectral analysis. All compounds evaluated for their cytotoxic effects four cell lines by MTT method. Penicillenols A1 B1 showed cytotoxicities against HL-60 line IC50 values 0.76 μM 3.20 μM, respectively.

Eight drimane sesquiterpenes (1-8), six isochromane derivatives (9-14), and three known compounds, daldinin B (15), 9alpha-hydroxy-6beta-[(2E,4E,6E)-octa-2,4,6-trienoyloxy]-5alpha-drim-7-en-11,12-olide (16), pergillin (17), were isolated from the EtOAc extract of marine-derived fungus Aspergillus ustus 094102. The structures new compounds elucidated on basis spectroscopic analysis. cytotoxic effects A549 HL-60 cell lines evaluated by SRB MTT methods. Ustusorane E (13) showed significant...

Four new depsipeptides, mirabamides E−H (1−4), and the known depsipeptide mirabamide C (5) have been isolated from sponge Stelletta clavosa, collected Torres Strait. The planar structures were determined on basis of extensive 1D 2D NMR HRESIMS. absolute configurations established by advanced Marfey's method, NMR, GC-MS. four compounds all showed strong inhibition HIV-1 in a neutralization assay with IC50 values 121, 62, 68, 41 nM, respectively.

As part of our ongoing chemical investigation biologically active metabolites from marine-derived fungi, four new polyphenols containing both phenolic bisabolane and diphenyl ether units, expansols C–F (<b>1</b>–<b>4</b>), one derivative, 3-<i>O</i>-methyldiorcinol (<b>5</b>), as well twelve known compounds (<b>6</b>–<b>17</b>), were isolated <i>Penicillium expansum</i> 091006 endogenous with the mangrove plant <i>Excoecaria agallocha</i> (Euphorbiaceae). The structures determined on basis...

Seven new ophiobolins (1-5, 12, and 14) along with the 11 known analogues (6-11, 13, 15-18) were isolated from ethyl acetate extracts of liquid solid cultures mangrove fungus Aspergillus ustus 094102. The structures including absolute configurations seven compounds elucidated by spectroscopic analysis, chemical methods, quantum ECD calculations. Compounds 4-8 11-15 showed cytotoxicities against G3K, MCF-7, MD-MBA-231, MCF/Adr, A549, HL-60 human cancer cell lines IC50 values ranging 0.6 to 9.5 μM.

To obtain structurally novel and bioactive natural compounds from marine-derived microorganisms, the effect of high salt stress on secondary metabolite production in fungal strain, Spicaria elegans KLA-03, was investigated. The organism, which isolated marine sediment, produced different metabolites when cultured 3% 10% saline conditions. Four characteristic metabolites, only salinity culture, were isolated, their structures identified as...

As part of our screening for anti-HIV agents from marine invertebrates, the MeOH extract Didemnum molle was tested and showed moderate in vitro activity. Bioassay-guided fractionation a large-scale allowed identification two new cyclopeptides, mollamides E F (1 2), one tris-phenethyl urea, molleurea A (3). The absolute configurations were established using advanced Marfey's method. three compounds evaluated activity both an HIV integrase inhibition assay cytoprotective cell-based assay....

Transient receptor potential (TRP) channels are activated by environmental particulate materials. We hypothesized that polymorphic variants of transient vanilloid-1 (TRPV1) would be uniquely responsive to insoluble coal fly ash compared with the prototypical soluble agonist capsaicin. Furthermore, these changes manifest as differences in lung cell responses agonists and perhaps correlate asthma symptom control. The TRPV1-I315M -T469I were more capsaicin ash. I585V variant was less particles...

To explore the association of trajectory serum Cystatin C (Cysc) with diabetic kidney disease (DKD), a retrospective cohort study Chinese subjects was carried out. A review 2,928 diabetes mellitus (DM) patients admitted to clinic and ward Endocrinology Department, Shengjing Hospital China Medical University from January 1, 2014 December 31, performed. Subsequent visits hospital were followed until 2020. The primary endpoint incidence DKD as diagnosed by urinary albumin/creatinine ratio ≥30...

Background: Transient receptor potential ankyrin-1 [transient cation channel subfamily A member 1 (TRPA1)] and vanilloid-1 V (TRPV1)] detect inhaled irritants, including air pollutants have roles in the development exacerbation of asthma. Objectives: This study tested hypothesis that increased expression TRPA1, stemming from loss-of-function TRPV1 (I585V; rs8065080) polymorphic variant by airway epithelial cells may explain prior observations worse asthma symptom control among children with...

Diesel exhaust particulate (DEP) causes pulmonary irritation and inflammation, which can exacerbate asthma other diseases. These effects may arise from the activation of transient receptor potential ankyrin-1 (TRPA1). This study shows that a representative DEP activate TRPA1-expressing C-fibers in mouse lung. Furthermore, collected idling vehicles at an emissions inspection station, tailpipe on-road "black smoker" diesel truck, waste filter regeneration machine, NIST SRM 2975 human TRPA1...

To better understand how adverse health effects are caused by exposure to particulate materials, and develop preventative measures, it is important identify the properties of particles molecular targets that link with specific biologic outcomes. Coal fly ash (CFA) a by-product coal combustion can affect human health. We report transient receptor potential melastatin-8 (TRPM8) an N-terminally truncated TRPM8 variant (TRPM8-Δ801) activated CFA calcium-rich nanoparticles and/or soluble salts...

Abstract Mi RNA s contribute greatly to epithelial mesenchymal transition ( EMT ) of peritoneal mesothelial cells PMC s), which is a crucial step in fibrosis PF ). In this study, we tried profile whether mi expression differences exist after human umbilical cord stem hUCMSC s) treatment rats and investigate the possible role miR‐153‐3p involved anti‐ process. We randomly assigned 34 into three groups: control group (Group Control), MGO ‐induced s‐treated + s). microarrays real‐time PCR...

We report an unusual 3-substituted pyridine polyketide, onydecalin A (1), which was obtained along with 2 as a major constituent from the fungus Aioliomyces pyridodomos (order: Onygenales) following two-month fermentation. Feeding studies demonstrated that subunit originates via unprecedented biosynthetic process in comparison to other polyketide-linked pyridines or derivatives such pyridones. The slow growth of led us perform one-year fermentation, leading production compounds 2–4...

Two ring-A-aromatized bile acids, 1 and 2, were isolated from the sponge Sollasella moretonensis, collected seabed of northern Queensland. Structures assigned on basis extensive 1D 2D NMR studies, as well analysis by HRESIMS. Compound 2 has previously been produced synthetically, though this marks its first isolation a natural source.

By means of bioassay-guided fractionation, a new steroidal alkaloid, plakinamine M (1), and the known compound, L (2), with unique acyclic side chain, were isolated from marine sponge Corticium sp. collected New Britain, Papua Guinea. The structures determined on basis extensive 1D 2D NMR HRESIMS. two compounds showed inhibition Mycobacterium tuberculosis MIC values 15.8 3.6 μg/mL, respectively.

Seven cyclic depsipeptides were isolated from Hapsidospora irregularis and structurally characterized as the calcium channel blocker leualacin six new analogues based on NMR HRESIMS data. These compounds named leualacins B–G. The absolute configurations of amino acids 2-hydroxyisocaproic determined by recording optical rotation values. Biological studies showed that influx elicited F in primary human lobar bronchial epithelial cells involves TRPA1 channel. Through genome sequencing targeted...

Four new isochromane derivatives (1–4) along with the known peniciphenol (5) and ( R)-2-(hydroxymethyl)-3-(2-hydroxypropyl)phenol (6) were isolated from EtOAc extract of fermentation broth mangrove fungus, Aspergillus ustus 094102. The structures compounds including absolute configuration elucidated on basis spectroscopic analysis, CD ECD calculation. Compounds 1 2 exhibited α-glucosidase inhibition anti-oxidation against DPPH radical IC 50 values 1.4 mM 25.7 μM, respectively.