A5101992912- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Ion Transport and Channel Regulation
- Alcohol Consumption and Health Effects
- Biosensors and Analytical Detection
- Cancer-related molecular mechanisms research
- High Altitude and Hypoxia
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Traumatic Brain Injury and Neurovascular Disturbances
- Heme Oxygenase-1 and Carbon Monoxide
- Advanced biosensing and bioanalysis techniques
- Prostate Cancer Diagnosis and Treatment
- Medicinal Plants and Bioactive Compounds
- Hippo pathway signaling and YAP/TAZ
- Galectins and Cancer Biology
- Dialysis and Renal Disease Management
- Angiogenesis and VEGF in Cancer
- Phytochemistry and Biological Activities
- Nuclear Receptors and Signaling
- Pregnancy and Medication Impact
- Urologic and reproductive health conditions
- Occupational and environmental lung diseases
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Chinese PLA General Hospital
2025
Beijing Proteome Research Center
2016-2025
Chinese People's Armed Police Force
2013-2024
Logistics University of People's Armed Police Force
2014-2024
Nanjing Medical University
2020
Jiangsu Province Hospital
2020
Pingjin Hospital
2017-2019
Necroptosis, a novel type of programmed cell death, is involved in stroke-induced ischemic brain injury. Although studies have sought to explore the mechanisms necroptosis, its signaling pathway has not yet be completely elucidated. Thus, we used oxygen-glucose deprivation (OGD) and middle cerebral artery occlusion (MCAO) models mimicking stroke (IS) conditions investigate necroptosis. We found that OGD MCAO induced local ischemia neurological deficit, while zVAD-fmk (zVAD, an apoptotic...
Mechanical stress which would cause deleterious adhesive effects on podocytes is considered a major contributor to the early progress of diabetic nephropathy (DN). Our previous study has shown that spironolactone could ameliorate podocytic capacity in rats. Autophagy been reported have protective role against renal injury. The present investigated underlying mechanisms by reduced damage under mechanical stress, focusing involvement autophagy. Human conditional immortalized exposed were...
Background & Aims: Only a minority of patients could benefit from systemic therapy owing to the high heterogeneity HCC. Therefore, deeper understanding pathogenesis HCC is essential for precision therapy. Genomic and proteomic studies have enhanced our However, phosphoproteomic characterization remains poorly understood. Approach Results: We conducted an in-depth analysis clinical cohort using high-coverage phosphoproteomic. Effective therapeutic targets were validated liver cancer cell...
Antimicrobial peptides (AMPs) are regarded as promising alternatives for antibiotics due to their inherent capacity prevent microbial drug resistance. Amphibians rich source of bioactive molecules, which provide numerous AMPs with various structures candidates. Here, we isolated and identified a novel AMP Brevinin-2Ta (B-2Ta) from the skin secretion European frog, Pelophylax kl. esculentus. In vitro studies revealed that it showed broad antimicrobial activities against S. aureus, E. coli C....
Early-onset preeclampsia (EOS-PE) refers to that occurred before 34 gestation weeks. This study is conducted explore the relationship between mitochondrial dysfunction and pathogenesis of EOS-PE using proteomic strategy.To identify altering expressed proteins severe healthy pregnancies, enrichment mitochondria coupled with iTRAQ-based quantitative method performed. Immunohistochemistry (IHC) western blot are performed detect alteration changing expression proteins, confirmed accuracy...
Hepatitis B virus X protein (HBx) participates in the occurrence and development processes of hepatocellular carcinoma (HCC) as a multifunctional regulation factor. However, underlying molecular mechanism remains obscure. Here, we describe use p21HBx/+ mouse SILAM (Stable Isotope Labeling Mammals) strategy to define pathological mechanisms for HBx induced liver cancer. We systematically compared series proteome samples from regular mice, 12- 24-month old mice representing inflammation HCC...
The skin is the largest organ of body and a potential route exposure to sunscreens cosmetics containing nanoparticles; however, permeability these nanoparticles currently unknown. In this paper, we studied transdermal delivery capacity through mouse water-soluble CdSeS quantum dots (QDs) deposition QDs in body. QD solution was coated onto dorsal hairless male ICR mice. Fluorescence microscopy transmission electron (TEM) were used observe distribution organs, inductively coupled plasma-mass...
In this work, a novel enzyme-linked immunosorbent assay (ELISA) with low limit of detection and high sensitivity was developed using atom transfer radical polymer (ATRP)-modified gold nanoparticles (AuNPs). Clear signal amplification achieved by introducing an abundance horseradish peroxidase (HRP) to the AuNPs, because ATRP modification. This result suggested that new ELISA able detect antigens in complex mixtures, (LOD) lower than conventional factor 81. The strategy greatly decreased LOD...
Glioma is one of the most malignant forms brain tumor, and has been persistent concern due to its high recurrence mortality rates, limited therapeutic options. As a cardiac glycoside, ouabain widespread applications in congestive heart diseases positive inotropic effect by inhibiting Na+/K+‑ATPase. Previous studies have demonstrated that antitumor activity several types human including glioma. However, exact underlying mechanism remains be elucidated. The purpose present study was elucidate...
Cinobufagin is a Na+/K+-ATPase (NKA) inhibitor with excellent anticancer effects to prolong the survival of patients. The purpose present study was clarify underlying mechanism cinobufagin using overexpression or inhibition aurora kinase A (AURKA) signaling. First, high expression alpha 1 subunit (ATP1A1) and AURAK resulted in increased malignant transformation hepatocellular carcinoma (HCC) patients cancer genome atlas (TCGA) data tissue samples. After treatment cinobufagin, we successfully...
Heme oxygenase‑1 (HO‑1), as an inducible and cytoprotective enzyme, has a protective effect against cellular oxidative stress. In the present study, cholesterol was used to induce lipid overload increase reactive oxygen species (ROS), leading stress in EA.hy926 cells. western blotting immunofluorescence analysis were detect expression level of important molecules metabolism process cholesterol. It confirmed that stimulation upregulated HO‑1 time‑dependent manner via activation translocation...
Cinobufagin, a cardiotonic steroid derived from toad venom extracts, exhibits significant anticancer properties by inhibiting Na[Formula: see text]/K[Formula: text]-ATPase in cancer cells. It is frequently used clinical settings to treat advanced-stage patients, improving their quality of life and survival time. However, its long-term use can result multidrug resistance other chemotherapy drugs, the exact mechanism underlying this effect remains unknown. Therefore, study explores molecular...
Heme oxygenase-1 (HO-1) is an inducible and cytoprotective enzyme that provides a defense against oxidant damage. The present study screened 137 HO-1/interacting proteins using profound co-immunoprecipitation (Co-IP) coupled with proteomics, profiled the global HO-1 interactome network, including oxidative phosphorylation, endoplasmic reticulum transport vesicle functions. Among these molecules, we observed novel interactor, emopamil-binding protein (EBP), closely related to cholesterol...
The Na+/K+‑ATPase inhibitor cinobufagin exhibits numerous anticancer effects on hepatocellular carcinoma (HCC) cells expressing wild‑type p53 via inhibition of aurora kinase A (AURKA) and activation signaling. However, the HCC mutant remain unclear. In present study, were investigated Huh‑7 with p53, AURKA overexpression or analyzed. Viability, cell cycle progression apoptosis determined using an MTT assay, flow cytometry Hoechst 33342 staining, respectively. expression levels p73...
Abstract Mi RNA s contribute greatly to epithelial mesenchymal transition ( EMT ) of peritoneal mesothelial cells PMC s), which is a crucial step in fibrosis PF ). In this study, we tried profile whether mi expression differences exist after human umbilical cord stem hUCMSC s) treatment rats and investigate the possible role miR‐153‐3p involved anti‐ process. We randomly assigned 34 into three groups: control group (Group Control), MGO ‐induced s‐treated + s). microarrays real‐time PCR...
Many studies have shown the Na+/K+-ATPase (NKA) might be a potential target for anticancer therapy. Cardiac glycosides (CGs), as family of naturally compounds, inhibited NKA activity. The present study investigates antitumor effect ouabain and elucidates pharmacological mechanisms CG activity in liver cancer HepG2 cell using SILAC coupled to LC–MS/MS method. Bioinformatics analysis 330 proteins that were changed cells under treatment with 0.5 μmol/L showed biological processes are associated...
Histone H3K27 demethylase Jumonji domain-containing protein 3 (JMJD3) is involved in somatic cell differentiation and tumor progression; however, the underlying mechanisms of JMJD3 cancer progression are yet to be fully explored. To improve understanding regarding function brain cells, present study investigated effects on epithelial-mesenchymal transition (EMT) migration glioma involving C-X-C motif chemokine ligand 12 (CXCL12)/C-X-C receptor 4 (CXCR4) axis. Immunohistochemical staining a...
Traumatic brain injury (TBI) contributes to hypocoagulopathy associated with prolonged bleeding and hemorrhagic progression. Bloodletting puncture therapy at hand twelve Jing-well points (BL-HTWP) have been applied as a first aid measure in various of emergent neurological diseases, but the detailed mechanisms modulation between central nervous system systemic circulation after acute TBI rodents remain unclear. To investigate whether BL-HTWP stimulation modulates hypocoagulable state exerts...
Zing finger protein 580 (ZFP580) is a novel Cys2-His2 zinc-finger transcription factor that has an anti-apoptotic role in myocardial cells. It involved the endothelial transforming growth factor‑β1 (TGF‑β1) signal transduction pathway as mothers against decapentaplegic homolog (Smad)2 binding partner. The aim of present study was to determine involvement ZFP580 TGF‑β1‑mediated cytoprotection chemical hypoxia‑induced apoptosis, using H9c2 cardiac myocytes. Hypoxia chemically induced cells by...
Activation of extracellular signal-regulated kinase (ERK) signalling, alteration the uterine microenvironment and a reduction in human chorionic gonadotrophin production have been linked with fetal trisomy 16-induced early embryonic death (EED). However, detailed biological mechanism EED remains unclear. Using quantitative proteomics we successfully screened differentially expressed proteins villous tissues from patients 16 (EEDT16), but normal chromosomes (EEDNC) undergoing elective...