A5024937354- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Crop Yield and Soil Fertility
- Genetics and Plant Breeding
- Genetic Mapping and Diversity in Plants and Animals
- MicroRNA in disease regulation
- Retinal Development and Disorders
- Neuroscience and Neuropharmacology Research
- Renal and related cancers
- Developmental Biology and Gene Regulation
- Cancer-related molecular mechanisms research
- Dermatoglyphics and Human Traits
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- Photoreceptor and optogenetics research
- PI3K/AKT/mTOR signaling in cancer
- Lipid metabolism and biosynthesis
- Parkinson's Disease Mechanisms and Treatments
- Phagocytosis and Immune Regulation
- Receptor Mechanisms and Signaling
- Mitochondrial Function and Pathology
- Bone and Dental Protein Studies
- Plant Water Relations and Carbon Dynamics
Inner Mongolia University
2019-2023
Yangzhou University
2016-2019
Shanghai Jiao Tong University
2006-2013
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
2013
Tianjin Central Hospital of Gynecology Obstetrics
2012
Institute of Crop Sciences
2010-2012
Chinese Academy of Agricultural Sciences
2010-2012
Cell Technology (China)
2012
Tianjin Medical University
2010
Ruijin Hospital
2009
Grain yields of eight representative semidwarf spring wheat ( Triticum aestivum L.) cultivars released in northwest Mexico between 1962 and 1988 have increased linearly across years as measured this region during 6 yr under favorable management irrigation. To understand the physiological basis progress possibly assist future selection for grain yield, leaf traits were determined 3 same study. Stomatal conductance g s ), maximum photosynthetic rate (A max , canopy temperature depression...
Four to five cycles of maize ( Zea mays L.) hybrid replacement have occurred in China since the 1970s. Retrospective analyses genetic yield may provide an understanding unexploited potential and indicate pathways for future improvement. The objective this study was assess gains grain yield, especially those due increased tolerance stress from 1970s through 2000s. Trials were conducted at two locations per year three densities location during 2005–2006 (Exp. 1) 2007–2008 2) growing seasons....
Mechanical stress which would cause deleterious adhesive effects on podocytes is considered a major contributor to the early progress of diabetic nephropathy (DN). Our previous study has shown that spironolactone could ameliorate podocytic capacity in rats. Autophagy been reported have protective role against renal injury. The present investigated underlying mechanisms by reduced damage under mechanical stress, focusing involvement autophagy. Human conditional immortalized exposed were...
An efficient genome editing approach had been established to construct the stable transgenic cell lines in domestic chicken (Gallus gallus domesticus) at present. Our objectives were investigate gene function differentiation process of embryonic stem cells (ESCs) into spermatogonial cells(SSCs). Three guides RNA (gRNAs) designed knockout Stra8 gene, and efficiency was evaluated using cleavage activity vitro transcription gRNA, Luciferase-SSA assay, T7 endonuclease I assay(T7E1) TA clone...
<b><i>Background:</i></b> Diabetic nephropathy (DN) is the leading cause of end-stage renal disease. Podocyte plays a key role in pathogenesis DN. Adhesive capacity damage podocytes characteristic Emerging evidence suggests that microRNAs (miRNAs) play crucial roles controlling many cell adhesion molecules thus contribute to normal adhesion. The miRNA podocytic adhesive diabetic conditions remain largely unknown. <b><i>Methods:</i></b> Diabetes...
Cattle have emerged as one of the most important domestic animals widely used for meat, milk, and fur. Derivation bovine pluripotent stem cells (PSCs) can be applied in drug selecting human disease modeling facilitated agriculture‐related applications such production genetically excellent cattle by gene editing. Extended PSCs (EPSCs), capable differentiating into embryonic extraembryonic parts, been generated mouse, human, pig. Whether EPSCs could generated, their chimeric competency remains...
Podocytes can respond to various injuries, including mechanical stress secondary diabetic nephropathy (DN), which may cause deleterious adhesive effects on podocytes. Integrin α3β1 is the major podocyte adhesion molecule. In this study, we aim investigate expression and identify differentially expressed microRNAs in podocytes under compared with normal cells treated spironolactone, respectively.Serum glucocorticoid induced kinase 1 (SGK1), mineralocorticoid receptor (MR) integrin were...
Purpose.: We investigated the potential of human parthenogenetic embryonic stem cells (hPESCs) to differentiate into RPE cells, and identified development-regulating microRNAs (miRNAs). Methods.: were derived from hPESCs. The expression markers miRNA profiles during differentiation studied by immunocytochemistry, real-time RT-PCR, array at three time points. Human fetal (hfRPE) also analyzed. target genes candidate miRNAs then validated. Results.: hPESC-derived exhibited similar morphology...
Abstract The present study established an efficient genome editing approach for the construction of stable transgenic cell lines domestic chicken (Gallus gallus domesticus). Our objectives were to facilitate breeding high-yield, high-quality strains, and investigate gene function in stem cells. Three guide RNA (gRNAs) designed knockout C2EIP gene, efficiency was evaluated DF-1 fibroblasts ESCs using luciferase single-strand annealing (SSA) recombination assay, T7 endonuclease I (T7EI) TA...
Summary. A recent genome‐wide association study discovered that two polymorphisms, interferon (IFN) alpha receptor 2 (IFNAR‐2) F8S and interleukin 10 (IL10RB) K47E, were associated with susceptibility to hepatitis B virus (HBV) infection in Africa. Here, we reevaluate the effects of polymorphisms on HBV Chinese Han population, extended look at their IFN response chronic (CHB). We included 341 patients CHB unrelated controls presenting asymptotic self‐limited infection, who well matched age...
Voltage-gated K(+) channel activities are involved in regulating growth factor-stimulated cell proliferation a variety of types. Here we report that suppression voltage-gated with 4-aminopyridine (4-AP), barium, and tetraethylammonium inhibited both EGF- insulin-stimulated myeloblastic leukemia ML-1 concentration-dependent manner. Both MAPK/ERK Akt pathways known to mediate proliferative signals factors including insulin. In serum-starved cells, insulin rapidly stimulated phosphorylation...
<b><i>Background/Aims: </i></b>Curcumin, a kind of plant polyphenolic compound, has been recently discovered to have renoprotective effects on diabetic nephropathy (DN). Podocyte can respond various injuries including mechanical stress secondary DN. Our previous study showed that podocyte miR-124 expression was up-regulated accompanied with podocytic adhesive capacity damage <i>in vitro</i> and vivo</i>. We hypothesized, in the present research...
Abstract Background 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induces Parkinson’s disease (PD)-like neurodegeneration of dopaminergic neurons in the substantia nigra pars compacta (SNpc) via its oxidized product, 1-methyl-4-phenylpyridinium (MPP+), which is transported by dopamine (DA) transporter into DA nerve terminals. receptor subtype 3 (D3 receptor) participates neurotransmitter transport, gene regulation system, physiological accommodation G protein-coupled superfamily...
Previous studies indicate that the CCCTC binding transcription factor (CTCF) regulates homeobox PAX6 gene in corneal epithelial cells. In present study, effect was investigated of CTCF activity on through interaction with five essential motifs located an 80-bp region upstream from P0 promoter.An electrophoretic mobility shift assay (EMSA) used to determine between and DNA motifs. mutagenesis applied identification motif functions. Immunohistochemistry Western blot analyses were performed...
Background Epigenetic regulation is critical for the maintenance of human pluripotent stem cells. It has been shown that cells, such as embryonic cells and induced appear to have a hypermethylated status compared with differentiated However, epigenetic differences in genes maintain stemness regulate reprogramming between remain unclear. Additionally, differential methylation patterns generated using diverse methods require further study. Methodology Here, we determined DNA profiles 10 cell...
A previous study demonstrated that CTCF (CCCTC binding factor) regulates homeobox Pax6 gene expression in early embryonic stages and plays a dominant role eye development. The purpose of the present was to explore further mechanism controlling human retinoblastoma (Rb) cells development chicken mouse retinas.Northern Western analyses were used detect expressions Rb cells. transcription reporter deletion mutants regulatory interaction between retina embryos. transgenic embryos mice...
Retrospective analyses of genetic yield may provide an understanding unexploited potential and indicate pathways for future improvement, could be the basis further study exploiting molecular mechanisms improvement in future. The objective this is to examine maize ( Zea mays L.) trends describe changes ear traits relationship between grain from 1950s 2000s China. Trials were conducted at three locations 2007 2008 five 2009. Twenty‐seven single‐cross hybrids, four double‐cross open‐pollinated...
Regulation of crucial lncRNAs involved in differentiation chicken embryonic stem cells (ESCs) to spermatogonia (SSCs) was explored by sequencing the transcriptome ESCs, primordial germ (PGCs) and SSCs with RNA-Seq; analytical bioinformatic methods were used excavate candidate lncRNAs. We detected expression PGCs forecasted related target genes. Utilizing wego, david string, function protein-protein interactions genes analyzed. Finally, based on string analysis, interaction diagrams relevant...