A5100407386- Microbial Natural Products and Biosynthesis
- Microbial metabolism and enzyme function
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Molecular Research
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Biochemical Acid Research Studies
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Microbial Metabolism and Applications
- Photosynthetic Processes and Mechanisms
- Porphyrin Metabolism and Disorders
- CRISPR and Genetic Engineering
- Algal biology and biofuel production
- Musculoskeletal synovial abnormalities and treatments
- Carbohydrate Chemistry and Synthesis
- Bone Metabolism and Diseases
- RNA and protein synthesis mechanisms
- Sarcoma Diagnosis and Treatment
- Virus-based gene therapy research
- GABA and Rice Research
- Biochemical and biochemical processes
- Natural product bioactivities and synthesis
- Biochemical Analysis and Sensing Techniques
Jiangnan University
2022-2025
Microbiology Institute of Shaanxi
2018-2024
Institute of Microbiology
2018-2024
The University of Melbourne
2021-2024
The Seventh Affiliated Hospital of Sun Yat-sen University
2024
Sun Yat-sen University
2014-2024
Chinese Academy of Sciences
2018-2024
University of Chinese Academy of Sciences
2018-2024
Peking University
2024
Peking University Third Hospital
2024
The titer of the microbial fermentation products can be increased by enzyme engineering. l-Sorbosone dehydrogenase (SNDH) is a key in production 2-keto-l-gulonic acid (2-KLG), which precursor vitamin C. Enhancing activity SNDH may have positive impact on 2-KLG production. In this study, computer-aided semirational design was conducted. Based analysis SNDH's substrate pocket and multiple sequence alignment, three modification strategies were established: (1) expanding entrance pocket, (2)...
Glycosylation can enhance the solubility and stability of flavonoids. The main limitation glycosylation process is low intracellular uridine diphosphate glucose (UDPG) availability. This study aimed to create a platform strain in Escherichia coli BL21(DE3) by multiple metabolic engineering UDPG supply. Glycosyltransferase TcCGT1 was introduced synthesize vitexin orientin from apigenin luteolin, respectively. To further expand this strain, not only were UDP rhamnose galactose synthesis...
Hydroxysalidroside is an important natural phenylethanoid glycoside with broad application prospects in the food and pharmaceutical fields. However, its low concentration plants complex extraction hinder production. Despite being a promising way to synthesize hydroxysalidroside Escherichia coli, glycosylation remains limiting factor for A de novo biosynthetic pathway was successfully constructed E. coli via screening of glycosyltransferase, overexpressing phosphoglucomutase (pgm) UDP-glucose...
Brasilamides E–J (1–6), the bisabolane sesquiterpenoids with 3-cyclohexylfuran (1 and 2) 3-cyclohexylfuranone (3–6) skeletons, were isolated from scale-up fermentation cultures of plant endophytic fungus Paraconiothynium brasiliense Verkley. Although brasilamide E (1) is a known metabolite, its structure elucidation has yet to be described. The structures 1–6 elucidated primarily by NMR experiments. Compounds 3–6 found racemic, 3 was further separated into enantiomers 3a 3b on chiral HPLC...
An efficient genome editing approach had been established to construct the stable transgenic cell lines in domestic chicken (Gallus gallus domesticus) at present. Our objectives were investigate gene function differentiation process of embryonic stem cells (ESCs) into spermatogonial cells(SSCs). Three guides RNA (gRNAs) designed knockout Stra8 gene, and efficiency was evaluated using cleavage activity vitro transcription gRNA, Luciferase-SSA assay, T7 endonuclease I assay(T7E1) TA clone...
Abstract Lung cancer is a lethal malignancy. Plenty of circular RNAs (circRNAs) have been identified to be the vital regulators in lung development. Here, we intended clarify functional role circRNA HIPK3 (circHIPK3, also called hsa_circ_0021593) and its underlying mechanism action. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed evaluate levels circHIPK3 miR-381-3p. Cell viability apoptosis rate were monitored by Counting Kit-8 assay flow cytometry,...
Butenolides are an emerging family of signaling molecules in Streptomyces. They control complex physiological traits, such as morphological differentiation and antibiotic production. However, how butenolides regulate these processes is poorly investigated because obstacles obtaining molecules. This study reports the identification a butenolide-type system for nikkomycin biosynthesis Streptomyces ansochromogenes with distinct features. We identified gene cluster, sab, consisting three genes,...
Abstract L‐Sorbosone dehydrogenase (SNDH) is a key enzyme involved in the biosynthesis of 2‐keto‐L‐gulonic acid , which direct precursor for industrial scale production vitamin C. Elucidating structure and catalytic mechanism essential improving SNDH performance. By solving crystal structures from Gluconobacter oxydans WSH‐004, reversible disulfide bond between Cys295 Cys296 residues discovered. It allowed to switch oxidation reduction states, resulting opening or closing substrate pocket....
Gluconobacter oxydans is an important Gram-negative industrial microorganism that produces vitamin C and other products due to its efficient membrane-bound dehydrogenase system. Its incomplete oxidation system has many crucial applications. However, it also leads slow growth low biomass, requiring further metabolic modification for balancing the cell process. As a non-model strain, G. lacks genome editing tools cannot perform rapid multi-gene complex network regulation. In last 15 years, our...
Coniferyl alcohol, a fundamental component of lignin, serves as precursor for the biosynthesis lignan compounds. However, low content free coniferyl alcohol limits its production via plant extraction. Besides, demand multiple cofactors, complex and inefficient pathways, metabolite toxicity constrained using microorganisms. To overcome these shortcomings, flexible linkers efflux proteins encoded by srpB were used to optimize in Escherichia coli. After optimization biosynthetic pathway, NADPH...
Long-term and high-dose glucocorticoids (GCs) supplementation has been linked to osteoporosis. In this study, we studied the protective role of plumbagin against GC-induced cell damage in MC3T3-E1 cells. The effect dexamethasone (DEX) on viability was determined. DEX showed as IC-50 value 95 μM. Further, 10 μM treatment effectively ameliorated DEX-induced death by increasing 92 %. A further oxidative stress determined through reactive oxygen species (ROS) level, lipid peroxide content,...
Neomycin, a multicomponent aminoglycoside antibiotic, is mainly utilized in livestock husbandry and feed additives animals. The antimicrobial potency of the main product neomycin B higher than that its stereoisomer C. However, content C as an impurity high-producing strain relatively high, isolation or removal from quite difficult, which influences widespread application neomycin. In this work, essential genes responsible for biosynthesis were evaluated overexpressed to reduce Among them,...