A5101740721- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Microbial metabolism and enzyme function
- Fungal and yeast genetics research
- Glycosylation and Glycoproteins Research
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- Proteoglycans and glycosaminoglycans research
- Polyamine Metabolism and Applications
- RNA and protein synthesis mechanisms
- Microbial Metabolites in Food Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Plant biochemistry and biosynthesis
- Biochemical Acid Research Studies
- Phytase and its Applications
- Hemoglobin structure and function
- Porphyrin Metabolism and Disorders
- Fermentation and Sensory Analysis
- Cancer Research and Treatments
- Probiotics and Fermented Foods
- Animal Genetics and Reproduction
Jiangnan University
2016-2025
State Key Laboratory of Food Science and Technology
2009-2025
Jiangsu Industry Technology Research Institute
2025
Qingdao Academy of Agricultural Sciences
2020-2024
Shaanxi University of Science and Technology
2024
Jiaxing University
2024
Nanjing Tech University
2024
Zhejiang A & F University
2024
Xuzhou Medical College
2015-2023
First People's Hospital of Kunshan
2023
Abstract High-sugar diet causes health problems, many of which can be addressed with the use sugar substitutes. Rubusoside and rebaudiosides are interesting molecules, considered next generation substitutes due to their low-calorie, superior sweetness organoleptic properties. However, low abundance in nature makes traditional plant extraction process neither economical nor environmental-friendly. Here we engineer baker’s yeast Saccharomyces cerevisiae as a chassis for de novo production...
Dynamic regulation is an effective strategy for fine-tuning metabolic pathways in order to maximize target product synthesis. However, achieving dynamic and autonomous up- down-regulation of the modules interest simultaneously, still remains a great challenge. In this work, we created dual-control (ADC) system, by combining CRISPRi-based NOT gates with novel biosensors key metabolite pathway interest. By sensing levels intermediate glucosamine-6-phosphate (GlcN6P) self-adjusting expression...
Abstract 5-Aminolevulinic acid (ALA), the committed intermediate of heme biosynthesis pathway, shows significant promise for cancer treatment. Here, we identified that in addition to hemA and hemL , hemB hemD hemF hemG hemH are also major regulatory targets pathway. Interestingly, up-regulation benefited ALA accumulation whereas overexpression diminished accumulation. Accordingly, by combinatorial with different copy-number plasmids, titer was improved 3.25 g l −1 . Furthermore, combination...
Due to increasing concerns about food safety and environmental issues, bio-based production of flavonoids from safe, inexpensive, renewable substrates is increasingly attracting attention. Here, the complete biosynthetic pathway, consisting 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase (DAHPS), chorismate mutase/prephenate dehydrogenase (CM/PDH), tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone (CHS), isomerase (CHI), malonate synthetase, carrier protein, was...
Chicken feather, a byproduct of poultry-processing industries, are considered potential high-quality protein supplement owing to their crude content more than 85%. Nonetheless, chicken feathers have been classified as waste because the lack effective recycling methods. In our previous studies, Bacillus licheniformis BBE11-1 and Stenotrophomonas maltophilia shown feather-degrading capabilities in qualitative phase. To efficiently recycle feather waste, this study, we investigated...
(2S)-Naringenin, a (2S)-flavanone, is widely used in the food, chemical, and pharmaceutical industries because of its diverse physiological activities. The production (2S)-naringenin microorganisms provides an ideal source that reduces cost flavonoid. To achieve efficient Saccharomyces cerevisiae (S. cerevisiae), we constructed biosynthetic pathway from p-coumaric acid, cost-effective more precursor. synthesis genes were integrated into yeast genome to obtain strain. After gene dosage...
Pathway optimization plays an important role in fine-tuning metabolic pathways. In most conditions, more than three genes are involved the biosynthesis pathway of a specific target product. To improve titer products, rational regulation group by series promoters with different strengths is essential. On basis RNA-Seq data, set 66 native was chosen to fine-tune gene expression Saccharomyces cerevisiae. Promoter strength characterized measuring fluorescence enhanced green fluorescent protein...
Abstract Hyaluronan is widely used in cosmetics and pharmaceutics. Development of robust safe cell factories cultivation approaches to efficiently produce hyaluronan many interests. Here, we describe the metabolic engineering Corynebacterium glutamicum application a fermentation strategy manufacture with different molecular weights. C. engineered by combinatorial overexpression type I synthase, enzymes intermediate pathways attenuation extracellular polysaccharide biosynthesis. The strain...