A5101878133- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Probiotics and Fermented Foods
- Plant Gene Expression Analysis
- Bacterial Genetics and Biotechnology
- Protease and Inhibitor Mechanisms
- Galectins and Cancer Biology
- Meat and Animal Product Quality
- Protein Hydrolysis and Bioactive Peptides
- Polysaccharides and Plant Cell Walls
- Computational Drug Discovery Methods
- Protein Tyrosine Phosphatases
- Muscle metabolism and nutrition
- CRISPR and Genetic Engineering
- Enzyme Catalysis and Immobilization
- Proteins in Food Systems
- Microbial Metabolites in Food Biotechnology
- Inflammasome and immune disorders
- Amino Acid Enzymes and Metabolism
- Biofuel production and bioconversion
- Photosynthetic Processes and Mechanisms
- Alzheimer's disease research and treatments
- Microbial Metabolism and Applications
First Affiliated Hospital of Jiangxi Medical College
2025
Jiangxi Provincial People's Hospital
2025
Jiangnan University
2012-2024
Fujian Normal University
2024
Nanjing Agricultural University
2016-2023
Nanjing Forestry University
2022
Southwest University
2019
Jiangsu Academy of Agricultural Sciences
2016-2017
The Synergetic Innovation Center for Advanced Materials
2014
State Key Laboratory of Food Science and Technology
2012
Abstract The limited supply of intracellular malonyl-CoA in Escherichia coli impedes the biological synthesis polyketides, flavonoids and biofuels. Here, a clustered regularly interspaced short palindromic repeats (CRISPR) interference system was constructed for fine-tuning central metabolic pathways to efficiently channel carbon flux toward malonyl-CoA. Using synthetic sgRNA silence candidate genes, genes that could increase level by over 223% were used as target genes. efficiencies...
Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of the insulin and leptin receptor pathways thus an attractive therapeutic target for diabetes obesity. Starting with high micromolar lead compound, structure-based optimization novel PTP1B inhibitors by extension molecule from enzyme active site into second phosphotyrosine binding described. Medicinal chemistry, guided X-ray complex structure molecular modeling, has yielded low nanomolar in efficient manner. Compounds this...
Due to increasing concerns about food safety and environmental issues, bio-based production of flavonoids from safe, inexpensive, renewable substrates is increasingly attracting attention. Here, the complete biosynthetic pathway, consisting 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase (DAHPS), chorismate mutase/prephenate dehydrogenase (CM/PDH), tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone (CHS), isomerase (CHI), malonate synthetase, carrier protein, was...
Matrix metalloprotease 12 plays a significant role in airway inflammation and remodeling. Increased expression production of MMP-12 have been found the lung human COPD patients. MMP408 (14), potent selective inhibitor, was derived from matrix 2 13 inhibitor via lead optimization has good physical properties bioavailability. The compound blocks rhMMP-12-induced mouse model advanced for further development treatment COPD.
The health benefits of flavonoids for humans are increasingly attracting attention. Because the extraction high-purity from plants presents a major obstacle, interest has emerged in biosynthesizing them using microbial hosts. Eriodictyol is flavonoid with anti-inflammatory and antioxidant activities. Its efficient synthesis been hampered by two factors: poor expression cytochrome P450 low intracellular malonyl coenzyme A (malonyl-CoA) concentration Escherichia coli. To address these issues,...
Malonyl coenzyme A (malonyl-CoA) is an important precursor for the synthesis of natural products, such as polyketides and flavonoids. The majority this cofactor often consumed producing fatty acids phospholipids, leaving only a small amount cellular malonyl-CoA available target compound. tuning into heterologous pathways yields significant phenotypic effects, growth retardation even cell death. In study, fine-tuning acid pathway in Escherichia coli with antisense RNA (asRNA) to balance...
BACE1 is an aspartyl protease responsible for cleaving amyloid precursor protein to liberate Abeta, which aggregates leading plaque deposits implicated in Alzheimer's disease. We have identified small-molecule acylguanidine inhibitors of BACE1. Crystallographic studies show that these compounds form unique hydrogen-bonding interactions with the catalytic site aspartic acids and stabilize a flap-open conformation. Structure-based optimization led identification potent analogs, such as 10d...
Abstract A growing demand for sustainable, alternative protein sources that are nutrient‐dense, such as microorganisms, and insects, has gradually evolved. When paired with effective processing techniques, yeast cells contain substantial substances could supply the population's needs food, medicine, fuel. This review article explores potential of proteins a sustainable viable to animal plant‐based sources. It highlights various extraction methods including both mechanical non‐mechanical...
Postoperative liver failure due to insufficient cell quantity and function remains a major cause of mortality following surgery. Hence, additional investigation elucidation are required concerning suitable surgeries for promoting in vivo regeneration. We established the portal vein ligation (PVL) associated partition staged hepatectomy (ALPPS) mouse models compare their regeneration capacity. Then, RNA-seq microRNA-seq were conducted on livers from both models. Weighted gene co-expression...
In this study, novel fermented chickpea milk with high γ -aminobutyric acid (GABA) content and potential neuroprotective activity was developed. Fermentation starter that can produce GABA selected from 377 strains of lactic bacteria isolated traditional Chinese foods. Among the screened strains, strain M-6 showed highest GABA-producing capacity in De Man-Rogosa Sharp (MRS) broth milk. identified as Lactobacillus plantarum based on Gram staining, API carbohydrate fermentation pattern testing,...
Abstract A grand challenge of biological chemical production is the competition between synthetic circuits and host genes for limited cellular resources. Quorum sensing (QS)-based dynamic pathway regulations provide a pathway-independent way to rebalance metabolic flux over course fermentation. Most cases, however, these strategies only have capacity single QS circuit functional in one cell. Furthermore, current mainly localized control flux. Here, with aid engineering orthogonal...