A5100343995- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Marine Sponges and Natural Products
- Inflammatory mediators and NSAID effects
- Synthetic Organic Chemistry Methods
- Chemical synthesis and alkaloids
- Plant Pathogens and Fungal Diseases
- Cancer Treatment and Pharmacology
- Microbial Metabolism and Applications
- Nanoplatforms for cancer theranostics
- Plant biochemistry and biosynthesis
- Autophagy in Disease and Therapy
- Estrogen and related hormone effects
- Natural product bioactivities and synthesis
- Chemical Synthesis and Analysis
- DNA Repair Mechanisms
- Antifungal resistance and susceptibility
- Cancer, Stress, Anesthesia, and Immune Response
- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Sesquiterpenes and Asteraceae Studies
- Fungal Infections and Studies
- Phytochemical compounds biological activities
- Healthcare and Venom Research
- Traditional and Medicinal Uses of Annonaceae
University of Chinese Academy of Sciences
2021-2025
Chinese Academy of Sciences
2015-2025
Institute of Microbiology
2015-2025
East China University of Science and Technology
2022-2025
Chengdu University of Traditional Chinese Medicine
2022-2024
Yibin University
2024
Harbin Medical University
2024
Zhejiang Cancer Hospital
2022-2023
Wuhan University
2022
Zhejiang University
2022
In recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also commonly isolated endophyte which an important source bioactive natural products. fici Steyaert W106-1/CGMCC3.15140 tea produces numerous novel secondary metabolites, including chloropupukeananin, derivative chlorinated pupukeanane that first discovered in fungi. Some them might be drug leads for future pharmaceutics. Here, we report genome...
Chloropupukeananin (1), the first pupukeanane chloride with highly functionalized tricyclo-[4.3.1.03,7]-decane skeleton and its possible biosynthetic precursors iso-A82775C (2) pestheic acid (3), have been isolated from plant endophyte Pestalotiopsis fici. The structure of 1 was determined by NMR spectroscopy X-ray crystallography. Biogenetically, could be derived Diels−Alder adduct 2 3.
Chloropestolide A (1), a highly functionalized spiroketal with an unprecedented skeleton derived from chlorinated bicyclo-[2.2.2]-oct-2-en-5-one ring and 2,6-dihydroxy-4-methylbenzoic acid unit, has been isolated the scale-up fermentation extract of Pestalotiopsis fici. The structure 1 was elucidated by NMR spectroscopy X-ray crystallography. could be same Diels−Alder precursors as 2 showed significant inhibitory effects on growth two human cancer cell lines, HeLa HT29, GI50 values 0.7 4.2...
Water stress is one of the major environmental stresses causing growth retardation and yield loss plants. In past decades, osmotic adjustment, antioxidant protection, stomatal movement have been extensively studied, but much less attention has paid to study root system reprogramming maximize water absorption survival under stress. Here, it shown that polyethylene glycol (PEG)-simulated mild moderate induced premature differentiation apical meristem (RAM). It demonstrated RAM a conserved...
Abstract Chloropupukeanolides C–E ( 8 – 10 ), three highly functionalized secondary metabolites featuring a novel spiroketal skeleton derived from the chlorinated tricyclo‐[4.3.1.0 3, 7 ]‐decane (pupukeanane) and 2,6‐dihydroxy‐4‐methylbenzoic acid moieties, were isolated scale‐up fermentation extract of plant endophytic fungus Pestalotiopsis fici . The constitutions compounds elucidated primarily by NMR experiments. Their relative configurations deduced analogy to 4 6 , which previously same...
We previously established that COX ‐2 overexpression promotes breast cancer progression and metastasis. As long‐term use of inhibitors ( ‐2i) can promote thrombo‐embolic events, we tested an alternative target, prostaglandin E2 receptor EP 4 subtype 4), downstream ‐2. Here used the highly metastatic syngeneic murine C3L5 model to test role 4‐expressing macrophages in vascular endothelial growth factor VEGF )‐C/D production, angiogenesis, lymphangiogenesis situ , stem‐like cell SLC )...
Fungal highly reducing polyketide synthases (HRPKSs) are programmed multidomain enzymes that synthesize reduced structures. Recent reports indicated salicylaldehydes synthesized by HRPKS biosynthetic gene clusters, which unexpected based on known enzymology of HRPKSs. Using genome mining a Trichoderma virens cluster encodes number redox enzymes, we uncover the strategy used pathways in biosynthesis aromatic products such as salicylaldehyde 4, can be oxidatively modified to epoxycyclohexanol...
Oxosorbicillinol and cage-like acresorbicillinol C are bioactive sorbicillinoids produced by Acremonium chrysogenum. We found that a berberine bridge enzyme-like oxidase AcsorD was responsible for their biosynthesis gene deletion heterologous expression. catalyzed oxidation of sorbicillinol to form oxosorbicillinol in vitro assays, which successively condensed with spontaneously. Finally, site-directed mutation revealed Tyr525 the key residue catalysis reaction unlocking production.
Chloropupukeanolides A (1) and B (2), unprecedented spiroketal peroxides, chloropupukeanone (3), three highly functionalized metabolites featuring a chlorinated pupukeanane core, were isolated from an endophytic fungus Pestalotiopsis fici, with 1 showing significant anti-HIV-1 cytotoxic effects.
Abstract Background Essential hypertension (EH) is a complex disease as consequence of interaction between environmental factors and genetic background, but the pathogenesis EH remains elusive. The emerging tools network medicine offer platform to explore at system level. In this study, we aimed identify key proteins biological regulatory pathways involving in further molecular connectivities these by topological analysis Protein-protein (PPI) network. Result extended including one giant...
Protein N-arginine methyltransferases (PRMTs) participate in a number of cellular processes, including cell growth, nuclear/cytoplasmic protein shuttling, differentiation, RNA splicing and post-transcriptional regulation. PRMT2 (also known as HRMT1L1) is clearly involved lung function, the inflammatory response, apoptosis promotion, Wnt signaling leptin regulation through different mechanisms. In this study, we report molecular biological characterization three novel splice variants isolated...
MicroRNAs (miRs) are small regulatory molecules emerging as potential biomarkers in cancer. Previously, it was shown that COX-2 expression promotes breast cancer progression via multiple mechanisms, including induction of stem-like cells (SLC), owing to activation the prostaglandin E2 receptor EP4 (PTGER4). overexpression also upregulated microRNA-526b (miR-526b), association with aggressive phenotype. Here, functional roles miR-526b and mechanistic role signaling upregulation were examined....
Abstract The diphenyl ether pestheic acid was isolated from the endophytic fungus Pestalotiopsis fici , which is proposed to be biosynthetic precursor of unique chloropupukeananes. gene ( pta ) cluster identified in through genome scanning. Sequence analysis revealed that this encodes a nonreducing polyketide synthase, number modification enzymes, and three regulators. Gene disruption intermediate demonstrated biosynthesis proceeded formation backbone, cyclization polyketo benzophenone,...
Candida auris is an emerging pathogenic fungal species found worldwide. Since April 2016, C. colonization/infection cases have been in a general hospital Shenyang, China. The genome-based phylogenetic studies of these isolates remain undefined. In the current study, microbiological characteristics and antifungal susceptibility isolates, which were collected Shenyang during three-year period (2016–2018), investigated. Whole-genome sequencing was applied to investigate genetic variation...
Macrofungi are well-known as edible-medicinal mushrooms, which belong mostly to Basidiomycota, with a few from Ascomycota. In recent years, macrofungi have been recognized rich resource of structurally unique secondary metabolites, demonstrating wide range bioactivities, including anti-tumor, antioxidant, anti-inflammatory, antimicrobial, antimalarial, neuro-protective, hypoglycemic, and hypolipidemic activities. This review highlights over 270 natural products produced by 17 families...
Zanthoxylum nitidum (Roxb.) DC. (Z. nitidum) is a traditional Chinese medicinal plant that indigenous to the southern regions of China. Previous research has provided evidence significant anti-inflammatory, antibacterial, and anticancer properties exhibited by Z. nitidum. The potential therapeutic effects cardiac toxicity remain uncertain. aim this was investigate four main compounds in cardiovascular diseases, their impact on electrical activity cardiomyocytes, underlying mechanism...
The NLRP3 inflammasome has been recognized as a promising therapeutic target in drug discovery for inflammatory diseases. Our initial research identified natural sesquiterpene isoalantolactone (IAL) the active scaffold targeting inflammasome. To improve its activity and metabolic stability, total of 64 IAL derivatives were designed synthesized. Among them, compound
Abstract Cephalosporin C (CPC) is a critical raw material for cephalosporin antibiotics produced by Acremonium chrysogenum . During fermentation, the oxygen supply crucial factor limiting efficient biosynthesis of CPC. This study demonstrated that addition exogenous surfactants significantly increased dissolved (DO) level, extracellular catalase content, and final CPC titer. Consequently, we hypothesized examined correlation between in A. through both hydrogen peroxide (H₂O₂) endogenous...
One new bergamotane-type sesquiterpeniod lactam brasilactam A (1) and one spiroketal lactone brasiketalolide (2), along with two known compounds ampullicin (3) isoampullicin (4) were isolated from the crude extract of plant endophytic fungus Paraconiothynium brasiliense Verkley. The structures elucidated on basis extensive 2D NMR techniques. absolute configurations 1 2 assigned by 13C electronic circular dichroism (ECD) calculations. Furthermore, − 4 tested for their cytotoxicity against...