A5036376546- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Probiotics and Fermented Foods
- Aquaculture Nutrition and Growth
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Pneumocystis jirovecii pneumonia detection and treatment
- Peptidase Inhibition and Analysis
- Aquaculture disease management and microbiota
- Polyamine Metabolism and Applications
- Yeasts and Rust Fungi Studies
- Gut microbiota and health
- Synthesis and Biological Evaluation
- Minerals Flotation and Separation Techniques
- Cancer-related molecular mechanisms research
- Carbohydrate Chemistry and Synthesis
- Plant-Microbe Interactions and Immunity
- Mechanical Failure Analysis and Simulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Antimicrobial Resistance in Staphylococcus
- Mineral Processing and Grinding
- Mechanical stress and fatigue analysis
- Reproductive biology and impacts on aquatic species
- Industrial Technology and Control Systems
Fudan University
2018-2025
Huashan Hospital
2019-2025
Southwest University
2023-2025
Guangxi Academy of Fishery Sciences
2018-2024
Shanghai Micro Satellite Engineering Center
2022-2024
State Key Laboratory of Genetic Engineering
2018-2024
Tongji University
2024
Shanghai Pulmonary Hospital
2024
South China University of Technology
2018-2023
Jiangxi Agricultural University
2023
Candida auris is an emerging multidrug-resistant fungal pathogen and a new global threat to human health. A unique morphological feature of this fungus its multicellular aggregating phenotype, which has been thought be associated with defects in cell division. In study, we report form two clinical C . isolates increased biofilm forming capacity due enhanced adherence adjacent cells surfaces. Unlike the previously reported morphology, can become unicellular after treatment proteinase K or...
Abstract Candida auris has become a serious threat to public health. The mechanisms of how this fungal pathogen adapts the mammalian host are poorly understood. Here we report rapid evolution an adaptive C. multicellular aggregative morphology in murine during systemic infection. cells accumulate brain and exhibit obvious advantages over single-celled yeast-form Genetic mutations, specifically de novo point mutations genes associated with cell division or budding processes, underlie...
The emerging human fungal pathogen Candida auris has become a serious threat to public health. This spread 10 provinces in China as of December 2023. Here we describe 312 C. auris-associated hospitalizations and 4 outbreaks healthcare settings from 2018 Three genetic clades have been identified during this period. Molecular epidemiological analyses indicate that introduced local transmission occurred multiple instances China. Most isolated (98.7%) exhibited resistance fluconazole, while only...
Candida albicans, a commensal organism and pathogen of humans, can switch stochastically between white phase an opaque without intermediate phase. The phases have distinct cell shapes gene expression programs. Once switched, each is stable for many divisions. White-opaque switching under a1-alpha2 repression therefore only happens in or alpha cells. Mechanisms that control the are unknown. Here, we identify Wor1 (white-opaque regulator 1) as master white-opaque switching. deletion WOR1...
To mate, the fungal pathogen Candida albicans must undergo homozygosis at mating-type locus and then switch from white to opaque phenotype. Paradoxically, cells were found be unstable physiological temperature, suggesting that mating had little chance of occurring in host, main niche C. albicans. Recently, however, it was demonstrated high levels CO(2), equivalent those host gastrointestinal tract select tissues, induced providing a possible resolution paradox. Here, we demonstrate second...
The emerging human fungal pathogen Candida auris has been recognized as a multidrug resistant species and is associated with high mortality. This fungus was first described in Japan 2009 reported at least 18 countries on five continents. In this study, we report the isolate of C. from bronchoalveolar lavage fluid (BALF) hospitalized woman China. Interestingly, susceptible to all tested antifungals including amphotericin B, fluconazole, caspofungin. Copper sulfate (CuSO4) also potent...
Non-genetic phenotypic variations play a critical role in the adaption to environmental changes microbial organisms. Candida albicans, major human fungal pathogen, can switch between several morphological phenotypes. This ability is for its commensal lifestyle and cause infections. Here, we report discovery of novel form C. referred as “gray” phenotype, which forms tristable switching system with previously reported white opaque White, gray, cell types differ number aspects including...
Morphological plasticity has historically been an indicator of increased virulence among fungal pathogens, allowing rapid adaptation to changing environments. Candida auris identified as emerging multidrug-resistant human pathogen global importance. Since the discovery this species, it thought that C. is incapable filamentous growth. Here, we report filamentation and three distinct cell types in auris: typical yeast, filamentation-competent (FC) cells. These form a novel phenotypic switching...
Phenotypic transitions play critical roles in host adaptation, virulence, and sexual reproduction pathogenic fungi. A minority of natural isolates Candida albicans, which are homozygous at the mating type locus (MTL, a/a or α/α), known to be able switch between two distinct cell types: white opaque. It is puzzling that white-opaque switching has never been observed majority C. albicans strains have heterozygous MTL genotypes (a/α), given they contain all opaque-specific genes essential for...
Abstract Candida auris is a multidrug-resistant human fungal pathogen responsible for nosocomial outbreaks worldwide. Although considerable progress has increased our understanding of the biological and clinical aspects C. , its interaction with host immune system only now beginning to be investigated in-depth. Here, we compare innate responses induced by BJCA001 albicans SC5314 in vitro vivo. Our results indicate that appears less immunoinflammatory than SC5314, this differential response...
The way in which signal transduction pathways evolve remains a mystery, primarily because we have few examples of ones that newly evolved. There are numerous how the same organism selectively share components, most notably between Saccharomyces cerevisiae for mating process, filamentation cell wall integrity, ascospore formation, and osmoregulation. These examples, however, not provided insights into such evolve. Here, through construction an overexpression library 107 transcription factors,...
Similar multicellular structures can evolve within the same organism that may have different evolutionary histories, be controlled by regulatory pathways, and play similar but nonidentical roles. In human fungal pathogen Candida albicans, a quite extraordinary example of this has occurred. Depending upon configuration mating type locus (a/α versus a/a or α/α), C. albicans forms alternative biofilms appear morphologically, exhibit dramatically characteristics are regulated distinctly signal...
Candida auris is a newly emerging fungal pathogen of humans and has attracted considerable attention from both the clinical basic research communities. Clinical isolates C. are often resistant to one or more antifungal agents. To explore how resistance develops, we performed experimental evolution assays using fluconazole-susceptible isolate (BJCA001). After series passages through medium containing increasing concentrations fluconazole, cells acquired resistance. By sequencing comparing...
The conserved cAMP-dependent protein kinase (PKA) plays critical roles in the regulation of morphological transitions and virulence human fungal pathogen Candida albicans. It has long been thought that PKA catalytic subunit is essential for cell viability this fungus. Paradoxically, single adenylyl cyclase-encoding gene, CYR1, which required production cAMP C. albicans, not growth. Here, a double mutant TPK1 TPK2 (tpk2/tpk2 tpk1/tpk1, t2t1), encode two isoforms was successfully generated,...
Morphological transitions and metabolic regulation are critical for the human fungal pathogen Candida albicans to adapt changing host environment. In this study, we generated a library of central pathway mutants in tricarboxylic acid (TCA) cycle, investigated functional consequences these gene deletions on C. biology. Inactivation TCA cycle impairs ability utilize non-fermentable carbon sources dramatically attenuates cell growth rates under several culture conditions. By integrating...
Candida albicans is the most common human fungal pathogen, causing not only superficial infections, but also life-threatening systemic disease. C. can grow in several morphological forms including unicellular yeast-form, elongated hyphae and pseudohyphae. In certain natural environments, exists as biofilms, which are structured surface-attached microbial communities. Transcription factors play a critical role morphogenesis biofilm development. this study, we identified four...
ABSTRACT Pathogenic fungi are capable of switching between different phenotypes, each which has a biological advantage. In the most prevalent human fungal pathogen, Candida albicans , phenotypic transitions not only improve its adaptation to continuously changing host microenvironment but also regulate sexual mating. this report, we show that tropicalis another important opportunistic undergoes reversible and heritable switching, referred as “white-opaque” transition. Here N...
Candida auris is an emerging pathogenic fungal species found worldwide. Since April 2016, C. colonization/infection cases have been in a general hospital Shenyang, China. The genome-based phylogenetic studies of these isolates remain undefined. In the current study, microbiological characteristics and antifungal susceptibility isolates, which were collected Shenyang during three-year period (2016–2018), investigated. Whole-genome sequencing was applied to investigate genetic variation...