A5053590002- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Probiotics and Fermented Foods
- 14-3-3 protein interactions
- Cancer-related Molecular Pathways
- Cellular transport and secretion
- Plant-Microbe Interactions and Immunity
- Carbohydrate Chemistry and Synthesis
- Microtubule and mitosis dynamics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Erythrocyte Function and Pathophysiology
- Adenosine and Purinergic Signaling
- Protein Degradation and Inhibitors
- Microbial Natural Products and Biosynthesis
- Venomous Animal Envenomation and Studies
- Pediatric health and respiratory diseases
- Fungal Biology and Applications
- T-cell and B-cell Immunology
- Photosynthetic Processes and Mechanisms
Center for Excellence in Molecular Cell Science
2015-2024
Chinese Academy of Sciences
2014-2024
MRC Laboratory of Molecular Biology
1995-2024
University of Chinese Academy of Sciences
2019-2023
Jiangsu University
2022
Shanghai Institutes for Biological Sciences
2002-2020
Jiangsu University of Technology
2011
Center for Excellence in Molecular Plant Sciences
2006
University of California, Irvine
1993-2002
Candida albicans, a commensal organism and pathogen of humans, can switch stochastically between white phase an opaque without intermediate phase. The phases have distinct cell shapes gene expression programs. Once switched, each is stable for many divisions. White-opaque switching under a1-alpha2 repression therefore only happens in or alpha cells. Mechanisms that control the are unknown. Here, we identify Wor1 (white-opaque regulator 1) as master white-opaque switching. deletion WOR1...
The transcription factor Flo8 is essential for filamentous growth in Saccharomyces cerevisiae and regulated under the cAMP/protein kinase A (PKA) pathway. To determine whether a similar pathway/regulation exists Candida albicans, we have cloned C. albicans FLO8 by its ability to complement S. flo8. Deleting blocked hyphal development hypha-specific gene expression. flo8/flo8 mutant avirulent mouse model of systemic infection. Genome-wide profiling efg1/efg1 using DNA microarray suggests that...
Summary Candida albicans had been thought to lack a mating process until the recent discovery of type‐like locus and between MTL MTLα strains. To elucidate molecular mechanisms that regulate in C. , we examined function Cph1 its upstream mitogen‐activated protein (MAP) kinase pathway mating, as they are homologues pheromone‐responsive MAP Saccharomyces cerevisiae . We found overexpressing CPH1 but not /α strains, induced transcription orthologues S. pheromone‐induced genes also increased...
Rabex-5 and Rabaptin-5 function together to activate Rab5 further promote early endosomal fusion in endocytosis. The GEF activity is autoinhibited by the CC domain (Rabex-5CC) activated C2-1 (Rabaptin-5C21) with yet unknown mechanism. We report here crystal structures of complex dimeric Rabaptin-5C21 (Rabaptin-5C212) Rabaptin-5C212 Rab5, along biophysical biochemical analyses. show that Rabex-5CC assumes an amphipathic α-helix which binds weakly substrate-binding site domain, leading weak...
Abstract Candida albicans can switch from commensal to pathogenic mode, causing mucosal or disseminated candidiasis. The host relies on pattern-recognition receptors including Toll-like (TLRs) and C-type lectin (CLRs) sense invading fungal pathogens launch immune defense mechanisms. However, the complex interplay between fungus innate immunity remains incompletely understood. Here we report that C . upregulates expression of a small secreted cysteine-rich protein Sel1 upon encountering...
MINT‐7147695, MINT‐7147713: pftk1 (uniprotkb:O94921) and CCNY (uniprotkb:Q8ND76) colocalize (MI:0403) by fluorescence microscopy (MI:0416)
Efg1 is essential for hyphal development and virulence in the human pathogenic fungus Candida albicans. How regulates gene expression unknown. Here, we show that interacts with components of nucleosome acetyltransferase H4 (NuA4) histone (HAT) complex both yeast cells. Deleting YNG2, a subunit NuA4 HAT module, results significant decrease acetylation level nucleosomal profound defect development, as well hypha-specific genes. Using chromatin immunoprecipitation, are found at UAS regions...
Candida albicans is associated with humans, as both a harmless commensal organism and pathogen. Adaption to human body temperature extremely important for its growth morphogenesis. Saccharomyces cerevisiae Esa1, member of the MYST family HATs (histone acetyltransferases) catalytic subunit NuA4 complex, homologues in other eukaryotes have been shown be essential cell growth. To investigate functional roles two HATs, Esa1 Sas2 C. albicans, we deleted ESA1 SAS2 genome performed analyses. Our...
Candida albicans, the most prevalent human fungal pathogen, can switch stochastically between white and opaque phases. In this study, we identified Zcf37, a zinc finger protein, as new regulator of white-opaque switching. Deletion ZCF37 increased white-to-opaque switching frequency stabilized state. Overexpression promoted conversion cells to phase, but needed existence Efg1, key required for maintenance EFG1 abolished effect ectopically expressed Zcf37 on opaque-to-white switching, whereas...
AbstractBoth mitogen-activated protein kinases and cyclin-dependent play a role in hyphal development Candida albicans. Using an oligonucleotide probe-based screen, we have isolated new member of the Cdc2 kinase subfamily, designated Crk1 (Cdc2-related kinase). The sequence is most similar to those Saccharomyces cerevisiae Sgv1 human Pkl1/Cdk9. InS. cerevisiae, CRK1 suppresses some, but not all, defects associated with sgv1 mutant. Deleting both copies C. albicansslows growth slightly leads...
ABSTRACT The ability to switch between different morphological forms is an important feature of Candida albicans and relevant its pathogenesis. Many conserved positive negative transcription factors are involved in morphogenetic regulation the two dimorphic fungi Saccharomyces cerevisiae. In S. cerevisiae , transcriptional repressor Sfl1 activator Flo8 function antagonistically invasive filamentous growth. We have previously reported that a factor essential for hyphal development virulence...
Cyclin Y family can enhance Wnt/β-catenin signaling in mitosis. Their physiological roles mammalian development are yet unknown. Here we show that Y-like 1 (Ccnyl1) and (Ccny) have overlapping function crucial for mouse embryonic mammary stem/progenitor cell functions. Double knockout of Ccnys results lethality at E16.5. In pubertal development, terminal end buds robustly express Ccnyl1. Depletion leads to reduction Lrp6 phosphorylation, hampering β-catenin activities abolishing expansion...
Abstract Phenotypic plasticity is common in development. Candida albicans , a polymorphic fungal pathogen of humans, possesses the unique ability to achieve rapid and reversible cell fate between unicellular form (yeast) multicellular (hypha) response environmental cues. The NuA4 histone acetyltransferase activity Hda1 deacetylase have been reported be required for hyphal initiation maintenance. However, how regulate elongation not clear. SWR1 chromatin remodeling complexes are conserved...
The ability of dimorphic transition between yeast and hyphal forms in Candida albicans is one the vital determinants for its pathogenicity virulence. We isolated C. SWI1 as a suppressor invasive growth defect Saccharomyces cerevisiae mutant. Expression S. partially complemented swi1 mutant utilization glycerol. Swi1 complex with Snf2 , both proteins are localized nucleus independent form. Deleting or SNF2 prevented true formation resulted constitutive pseudohypha‐like all media examined....
The Shu complex, a conserved regulator consisting of Csm2, Psy3, Shu1 and Shu2 in budding yeast, plays an important role the assembly Rad51–ssDNA filament homologous recombination. However, molecular basis for complex its functional DNA repair is still elusive. Here, we report crystal structure yeast revealing that interact with each other sequence to form V-shape overall structure. adopts resembling ATPase core domain Rad51 represents new paralog. assumes novel structural fold zinc-finger...
ABSTRACT Candida albicans undergoes a morphological transition from yeast to hyphae in response variety of stimuli and growth conditions. We previously isolated LisH domain containing transcription factor Flo8, which is essential for hyphal development C. . To search the putative binding partner Flo8 , we identified Mss11, functional homolog Saccharomyces cerevisiae also contains motif at its N terminus. Mss11 can interact with via by vivo coimmunoprecipitation. The results chromatin...
Many transcriptional regulators play roles in morphogenesis of the human pathogen Candida albicans. Recently, Sfl2, a sequence homolog C. albicans Sfl1, has been shown to be required for hyphal development. In this report, we show that, like Sfl2 could complement phenotypes Saccharomyces cerevisiae sfl1 mutant, and green fluorescent protein-tagged localized nuclei both yeast cells albicans, reflecting its role as regulator. SFL2 expression was induced at high growth temperature (37 °C)...
Abstract The morphological switch between yeast and hyphae of Candida albicans is essential for its interaction with the host defense system. However, lack understanding host–pathogen interactions during C. infection greatly hampers development effective immunotherapies. Here, we found that priming FLO8- deficient ( flo8 ) mutant, locked in form, protected mice from subsequent lethal infection. Deficiency Dectin-2, a fungus-derived α-mannan recognition receptor, completely blocked...
MRG15 is a transcription factor expressed in variety of human tissues, and its orthologs have been found many other eukaryotes which constitute the MRG protein family. It plays vital role embryonic development cell proliferation, involved cellular senescence. The C-terminal part forms conserved domain interactions with tumor suppressor retinoblastoma nucleoprotein PAM14 during transcriptional regulation. We report here characterization interaction between using both yeast two-hybrid vitro...
Candida albicans is a human pathogenic fungus which can undergo morphological transition from yeast to hyphae in response variety of environmental stimuli. We analyzed C. Asc1 (Absence growth Suppressor Cyp1) protein entirely composed seven repeats the WD domain, and conserved fungi metazoan. Deleting ASC1 led profound defect hyphal development under hypha-inducing conditions examined. Furthermore, deletion attenuated virulence mouse model systemic infection. These data strongly suggested...