A5041928806- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Asthma and respiratory diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Peptidase Inhibition and Analysis
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- interferon and immune responses
- NF-κB Signaling Pathways
- T-cell and B-cell Immunology
- Electrospun Nanofibers in Biomedical Applications
- Synthesis and Biological Evaluation
- Ion channel regulation and function
- Nail Diseases and Treatments
- Hemostasis and retained surgical items
- Nanoplatforms for cancer theranostics
- Signaling Pathways in Disease
- IL-33, ST2, and ILC Pathways
- Adenosine and Purinergic Signaling
- Immune cells in cancer
- Mesenchymal stem cell research
- Galectins and Cancer Biology
Shanghai Tenth People's Hospital
2020-2024
Tongji University
2015-2024
Second Military Medical University
2005-2024
Changhai Hospital
2009-2024
Shenyang Pharmaceutical University
2024
Hebei Medical University
2023
Shanghai Pulmonary Hospital
2016-2021
Shanghai Skin Disease Hospital
2018-2021
Shanghai East Hospital
2014
Abstract Aluminum-containing adjuvants have been used for nearly 100 years to enhance immune responses in billions of doses vaccines. To date, only a few approved use humans, among which aluminum-containing are the ones widely used. However, medical need potent and safe is currently continuously increasing, especially those triggering cellular cytotoxic T lymphocyte activation, urgently needed development efficient virus cancer Manganese an essential micronutrient required diverse biological...
Dectin-1 functions as a pattern recognition receptor for sensing fungal infection. It has been well-established that induces innate immune responses through caspase recruitment domain-containing protein 9 (CARD9)-mediated NF-κB activation. In this study, we find CARD9 is dispensable activation induced by ligands, such curdlan or Candida albicans yeast. contrast, regulates H-Ras linking Ras-GRF1 to H-Ras, which mediates Dectin-1-induced extracellular signal-regulated kinase (ERK) and...
Abstract Programmed death ligand 1 (PD-L1) has been shown to be inducibly expressed on neutrophils suppress host immunity during polymicrobial sepsis, virus and parasite infections. However, the role of PD-L1 neutrophil-mediated antifungal remains wholly unknown. Here, we show that expression murine human was upregulated upon engagement C-type lectin receptor Dectin-1 with its β-glucans, exposed fungal pathogen Candida albicans yeast. Moreover, β-glucan stimulation induced translocation into...
Abstract Cryptococcosis is a potentially lethal disease that primarily caused by the fungus Cryptococcus neoformans , treatment options for cryptococcosis are limited. Here, we show glucuronoxylomannan, major polysaccharide component of C. induces recruitment neutrophilic myeloid-derived suppressor cells in mice and patients with cryptococcosis. Depletion enhances host defense against infection. We identify C-type lectin receptor-2d recognizes glucuronoxylomannan to potentiate...
Introduction Patients with allergic bronchopulmonary aspergillosis (ABPA) suffer from repeated exacerbations. The involvement of T-cell subsets remains unclear. Methods We enrolled ABPA patients, asthma patients and healthy controls. T-helper type 1 (Th1), 2 (Th2) 17 (Th17) cells, regulatory T-cells (Treg) interleukin (IL)-21 + CD4 in total or sorted peripheral blood mononuclear cells bronchoalveolar lavage fluid (BALF) were analysed using flow cytometry. RNA sequencing was done exacerbated...
Activation of various C-type lectin receptors (CLRs) initiates potent proinflammatory responses against microbial infections. However, how activated CLRs are negatively regulated remains unknown. In this study, we report that activation Dectin-2 and Dectin-3 by fungi infections triggers them for ubiquitination degradation in a Syk-dependent manner. Furthermore, found E3 ubiquitin ligase Casitas B-lineage lymphoma protein b (Cbl-b) mediates the these through associating with each other via...
The incidence of Aspergillus fumigatus infection and the rate resistance to antifungal drugs have sharply increased in recent years. LL37 has been reported as a host defense peptide with broad-spectrum antibacterial activities. However, role during A. remains unclear. Here, we examined interaction between found that synthetic could directly bind surface fumigatus, disrupting integrity cell wall vitro. inhibited mycelial growth concentration-dependent manner, rather than fungicidal effect...
Background: There is limited understanding of the effect salt types on progression cardiovascular disease (CVD) and their longevity, due to previous evidence from cross-sectional studies. The purpose this study was evaluate association type with CVD all-cause mortality in American population by using a prospective design. Methods: National Health Nutrition Examination Survey (NHANES) large, complex, public health survey US population. Weighted Cox proportional hazard ratios (HRs) 95%...
Trehalose is a non-reducing disaccharide and can be accumulated in response to heat or oxidative stresses Candida albicans. Here we showed that C. albicans tps1Δ mutant, which deficient trehalose synthesis, exhibited increased apoptosis rate upon H2O2 treatment together with an increase of intracellular Ca2+ level caspase activity. When the was stimulated by adding CaCl2 A23187, both activity were increased. In contrast, presence two calcium chelators, EGTA BAPTA, could attenuate these...
Infection by invasive fungi, such as Candida albicans, Aspergillus fumigatus, and Cryptococcus neoformans, is one of the leading death causes for increasing population immunocompromised immunodeficient patients. Several C-type lectin receptors (CLRs), including Dectin-1, -2, -3 Mincle can recognize fungal surface components initiate host antifungal immune responses. Nevertheless, it remains to be determined whether other CLRs are involved in immunity. Our recent study suggests that CD23...
c-Cbl suppresses RelB activation to facilitate protective benefits of commensal fungi and maintain intestinal homeostasis.
Abstract Spleen tyrosine kinase (SYK) is a non-receptor kinase, which plays an essential role in both innate and adaptive immunity. However, the key molecular mechanisms that regulate SYK activity are poorly understood. Here we identified E3 ligase TRIM31 as crucial regulator of activation. We found interacted with catalyzed K27-linked polyubiquitination at Lys375 Lys517 SYK. This promoted its plasma membrane translocation binding C-type lectin receptors (CLRs), also prevented interaction...
Dimerization of C-type lectin receptors (CLRs) or Toll-like (TLRs) can alter their ligand binding ability, thereby modulating immune responses. However, the possibilities and roles dimerization between CLRs TLRs remain unclear. Here we show that receptor-2d (CLEC2D) forms homodimers, as well heterodimers with TLR2. Quantitative assays reveal both CLEC2D homodimers CLEC2D/TLR2 have a higher ability to fungi-derived β-glucans than TLR2 homodimers. Moreover, homo- hetero-dimeric mediates...
Multi-drug resistance of pathogenic microorganisms is becoming a serious threat, particularly to immunocompromised populations. The high mortality systematic fungal infections necessitates novel antifungal drugs and therapies. Unfortunately, with traditional drug discovery approaches, only echinocandins was approved by FDA as new class antifungals in the past two decades. Drug efflux one major contributors multi-drug resistance, modulator pumps considered keys conquer resistance. In this...
The γ-amino butyric acid (GABA)ergic system shapes the activation and function of immune cells. present study was conducted to explore regulation GABA transporter (GAT)-2 on differentiation Th17 Here we found that cells show higher abundance GAT-2, have distinct cellular metabolic signatures, such as shunt pathway, compared naïve T GAT-2 deficiency had little effect signature in cells, but impaired uptake pathway cell development peripheral homeostasis; however, its promoted vitro....
Cryptococcus neoformans and gattii cause life-threatening meningoencephalitis or pneumonia in immunocompetent individuals. C. are subdivided into five serotypes based on their capsular glucuronoxylomannan (GXM). consists of A, D, AD hybrid, B Given structural differences GXM between gattii, it remains unclear that how innate immune system recognizes GXM. Here, we report C-type lectin receptor Dectin-3 (MCL encoded by Clec4d) is a direct for GXMs from serotype (C.n-AD) (C.g-B). C.n-AD C.g-B...
Significance Each year, the global mortality rates for fungal diseases now exceed those malaria and breast cancer are currently comparable to tuberculosis HIV. The limited scope of available antifungal drugs is major factor underlying observed high rate. Here, we provide evidence that Myosin IF (MYO1F) plays a critical role in mediating signaling molecules “trafficking from membrane cytoplasm,” this process essential pathway activation. Moreover, Sirt2 deacetylase inhibitors promote immunity...