A5008911273- Plant nutrient uptake and metabolism
- Cellular transport and secretion
- Antifungal resistance and susceptibility
- Genetic Syndromes and Imprinting
- Plant tissue culture and regeneration
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Calcium signaling and nucleotide metabolism
- Click Chemistry and Applications
- Lipid Membrane Structure and Behavior
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Enzyme function and inhibition
- Virus-based gene therapy research
- Carbohydrate Chemistry and Synthesis
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- Phagocytosis and Immune Regulation
- Biopolymer Synthesis and Applications
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
The First Affiliated Hospital, Sun Yat-sen University
2025
Zhongshan Hospital
2025
Fudan University
2025
Sun Yat-sen University
2025
University of Tennessee at Knoxville
2021-2024
Knoxville College
2021-2024
Center for Excellence in Molecular Cell Science
2022
University of Chinese Academy of Sciences
2022
Nanjing Agricultural University
2021
Shenyang Agricultural University
2021
Abstract Background Depression is characterized by significant and low mood. Classical antidepressants are still not adequate in treating depression because of undesirable side effects. Folic acid, a member the vitamin B complex, considered to be strongly associated with function development central nervous system. Thus, this study, we established model through chronic unpredictable mild stress (CUMS) rats assessed antidepressant effects mechanisms folic acid. Methods Sprague–Dawley were...
Abstract Synuclein-γ (SNCG) plays oncogenic roles in breast carcinogenesis. Although the expression of SNCG is abnormally high advanced and metastatic carcinomas, not expressed normal or benign tissues. an intrinsically disordered protein known to interact with BubR1, a mitotic checkpoint kinase. The SNCG-BubR1 interaction inhibits control upon spindle damage caused by anticancer drugs, such as nocodazole taxol. Antimicrotubule drugs that cause arrest subsequent apoptosis cancer cells are...
Phosphatidylserine (PS) is a key lipid that plays important roles in disease-related biological processes, and therefore, the means to track PS live cells are invaluable. Herein, we describe metabolic labeling of Saccharomyces cerevisiae using analogues serine, precursor, derivatized with azide moieties at either amino (N-l-SerN3) or carbonyl (C-l-SerN3) groups. The conservative click tag modification enabled these compounds infiltrate normal biosynthetic pathways, thereby producing tagged...
Systemic infections by Candida spp. are associated with high mortality rates, partly due to limitations in current antifungals, highlighting the need for novel drugs and drug targets. The fungal phosphatidylserine synthase, Cho1, from albicans is a logical antifungal target its importance virulence, absence host, conservation among pathogens. Inhibitors of Cho1 could serve as lead compounds development, so we developed target-based screen inhibitors purified Cho1. This enzyme condenses...
Human Noroviruses (HuNoVs) are the main cause of nonbacterial gastroenteritis. Contaminated produce is a vehicle for dissemination HuNoVs. In this study, we used an ice nucleation protein mediated surface display system to present protruding domain GII.4 HuNoV capsid on bacterial and it as new strategy explore interaction between receptor candidates from romaine lettuce. The surface-displayed proteins were confirmed transformed bacteria by immunofluorescence assay. distribution patterns in...
Previous studies have revealed that biochar could induce the disturbance of a microbial community above family level. So far, very little is known about how individual bacteria are affected by at genus or species levels. In this study, effects and its water-soluble compounds on growth soil were examined. Biochar derived from different feedstock showed disproportionate impacts bacterial growth. Corncob significantly stimulate most tested strains, whereas four including Bacillus pumilus...
Poly-L-lactic acid (PLLA) is currently the most abundant bioplastic; however, limited environmental biodegradability and few recycling options diminish its value as a biodegradable commodity. Enzymatic one strategy for ensuring circularity of PLLA, but this approach requires thorough understanding enzymatic mechanisms protein engineering strategies to enhance activity. In study, we engineer PLLA depolymerizing subtilisin enzymes originating from Bacillus species elucidate molecular dictating...
Abstract We report the use of clickable monoacylglycerol (MAG) analogs as probes for labeling glycerolipids during lipid metabolism. Incorporation azide tags onto glycerol region was pursued to develop that would label glycerolipids, in which click tag not be removed through processes including acyl chain and headgroup remodeling. Analysis MAG containing chains different length resulted widely variable cell imaging cytotoxicity profiles. Based on these results, we focused a probe bearing...
Phosphatidylserine (PS) synthase from Candida albicans, encoded by the CHO1 gene, has been identified as a potential drug target for new antifungals against systemic candidiasis. Rational design or small molecule screening are effective ways to identify specific inhibitors of Cho1, but both will be facilitated protein purification. Due transmembrane nature methods were needed solubilize and purify native form Cho1. Here, we used six non-ionic detergents three styrene maleic acids (SMAs) an...
In this study, we present the probe SATE-G3P-N
Several pathways function to remove aberrant mRNA in eukaryotic cells; however, the exact mechanisms underlying restriction of transcription are poorly understood. In this study, we found that MORPHEUS' MOLECULE1 (MOM1) is a key component regulatory machinery. The Arabidopsis (Arabidopsis thaliana) mom1-44 mutation was identified by luciferase imaging transgenic plants harboring cauliflower mosaic virus 35S promoter-LUCIFERASE transgene lacking 3'-untranslated region. mutant, transcriptional...
The fungal phosphatidylserine (PS) synthase, a membrane protein encoded by the CHO1 gene, is potential drug target for pathogenic fungi, such as Candida albicans. However, both substrate-binding sites of C. albicans Cho1 have not been characterized. has two substrates: cytidyldiphosphate-diacylglycerol (CDP-DAG) and serine. Previous studies identified conserved CDP-alcohol phosphotransferase (CAPT) binding motif, which present within Cho1. We tested CAPT motif its role in PS synthesis...
Abstract The eukaryote, Dictyostelium discoideum, has one of the most (A+T) rich genomes studied to date. Isolated nuclear D. discoideum DNA (AX3 strain) was used qualitatively determine frequency and length distribution long (dA).(dT) homopolymer tracts in this genome, comparison less calf thymus Schistosoma mansoni DNAs that had few observable tracts. These experimental data accurately reflect significantly elevated frequencies found computationally within intron flanking sequences, but...
The imbalance between energy intake and expenditure leads to the prevalence of obesity worldwide. A strategy simultaneously limit promote would be an important new treatment. Here, we identified rhamnose as a nonnutritive sweetener adipose thermogenesis expenditure. Rhamnose promotes cAMP production PKA activation through dopamine receptor D1 in tissue. As result, administration UCP1-dependent ameliorates mice. Thus, have demonstrated rhamnose-dopamine D1-PKA axis critical for thermogenesis,...
Genomic imprinting is an epigenetic phenomenon mainly occurs in endosperm of flowering plants. Genome-wide identification imprinted genes have been completed several dicot Cruciferous plant and monocot crops.Here, we analyzed global patterns allelic gene expression developing sunflower which belongs to the composite family. Totally, 691 loci candidates were identified 12 day-after-pollination including 79 maternally expressed (MEG) 596 paternally (PEG), 6 noncoding RNAs (MNC) 10 (PNC). And a...
ABSTRACT The uridine derivatives UDP-glucose and UDP- N -acetylglucosamine are important for cell wall construction as they the precursors synthesis of β-1,3-glucan chitin, respectively. Previous studies have demonstrated attenuated virulence auxotrophs in mice, which has been attributed to insufficient levels growth host. We discovered that deprivation auxotroph ura3 ΔΔ disrupts architecture by increasing surface mannans, exposing decreasing UDP-sugar levels. Cell UDP-sugars can be rescued...
Abstract Systemic infections by Candida spp. are associated with high mortality rates, partly due to limitations in current antifungals, highlighting the need for novel drugs and drug targets. The fungal phosphatidylserine synthase, Cho1, from albicans is a logical antifungal target its importance virulence, absence host conservation among pathogens. Inhibitors of Cho1 could serve as lead compounds development, so we developed target-based screen inhibitors purified Cho1. This enzyme...
Systemic infections by Candida spp. are associated with high mortality rates, and this is related, in part, to limitations current antifungals. In regard, the phosphatidylserine (PS) synthase from albicans, encoded CHO1 gene, has been identified as a potential drug target for new The reasons include: 1) Cho1 required oral systemic infection mouse model essential viability fungal pathogen Cryptococcus neoformans; 2) gene product absent humans, 3) it highly conserved protein among many human...
Systemic infections by Candida spp. have high mortality rates, and this is related, in part, to limitations current antifungals. Thus, there a need for novel The phosphatidylserine (PS) synthase (annotated Cho1) from C. albicans has been identified as potential drug target because [1] it required virulence mouse models of Candidiasis, [2] absent humans, [3] highly conserved among fungal pathogens. Inhibitors Cho1 could be used lead compounds development. Rational design one way develop...