A5101567203- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Listeria monocytogenes in Food Safety
- Bacteriophages and microbial interactions
- Crystallography and molecular interactions
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Herpesvirus Infections and Treatments
- Child Nutrition and Water Access
- Salmonella and Campylobacter epidemiology
- Animal Disease Management and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Vector-Borne Animal Diseases
- Postharvest Quality and Shelf Life Management
- Viral Infections and Outbreaks Research
- Biochemical Analysis and Sensing Techniques
- Poxvirus research and outbreaks
- Hormonal and reproductive studies
- Pediatric health and respiratory diseases
- Vibrio bacteria research studies
Agricultural Research Service
2015-2024
Western Regional Research Center
2015-2024
United States Department of Agriculture
2015-2024
Hunan Agricultural University
2022
Center for Disease Control
2010
Human Immunome Project
2000
Baylor College of Medicine
1996
Ping An (China)
1991
Louisiana State University Health Sciences Center New Orleans
1985
Louisiana State University
1985
The rotavirus nonstructural glycoprotein NSP4 is an intracellular receptor that mediates the acquisition of a transient membrane envelope as subviral particles bud into endoplasmic reticulum. also causes increase in calcium insect cells. Purified or peptide corresponding to residues 114 135 induced diarrhea young (6 10 days old) CD1 mice. This disease response was age-dependent, dose-dependent, and specific. Electrophysiologic data from intestinal mucosa showed 114–135 potentiates chloride...
During a unique morphogenetic process, rotaviruses obtain transient membrane envelope when newly synthesized subviral particles bud into the endoplasmic reticulum (ER). As rotavirus mature, they lose their and layer of glycoprotein VP7 forms virion outer capsid shell. The nonstructural NSP4 functions as an intracellular receptor in ER (K. S. Au, W. K. Chan, J. Burns, M. Estes, Virol. 63:4553-4562, 1989), it has been hypothesized that is involved removal during viral morphogenesis (M. Estes...
Human histo-blood group antigens (HBGA) have been identified previously as candidate receptors for human norovirus (NOR). Type A, type H1, and Lewis HBGA in humans major NOR binding. We found that pig stomach (gastric) mucin (PGM) contains blood b binds to multiple strains of more broadly than do specific antibodies NOR. Both genogroup I (GGI) GGII were recovered by PGM-conjugated magnetic beads. A fecal sample containing was detected at a dilution 1:1,000,000 the standard RNA extraction...
To determine if histo-blood group antigens (HBGA) present in oyster gastrointestinal (GI) cells mediate accumulation of human noroviruses (NoV) GI cells.HBGA-specific monoclonal antibodies (MAbs) were used to the presence corresponding HBGA cells. All samples tested contained type A-like tissue as measured by ELISA. Recombinant Norwalk virus viral like particles (rNVLP) bound plates coated with homogenate. The binding was inhibited when rNVLPs pre-incubated MAbs specific for A HBGA, or...
To characterize the specificity and effect of pH ionic strength on kinetics virus binding to histo-blood group antigens (HBGA)-conjugated magnetic beads.HBGAs from porcine gastric mucin (PGM) have been conjugated beads (PGM-MB) for concentration NoV. A GII.4 was used detailed study a panel genogroup I (GI) NoVs, II (GII) NoVs recombinant (rNoVs) were efficiency assays. We determined that NoV can be captured after 15min incubation with PGM-MB, recovery is decreased extended times. rNoV as...
Folate plays essential role in sustaining cell activity, promoting growth, and participating division proliferation. The demand for colored wheat is increasing day by due to its high content of anthocyanin, iron, zinc, selenium, other beneficial elements. To investigate the folate derivatives grains wheat, this study employed a total 113 varieties (lines) with varying grain colors. four derivatives, tetrahydrofolate (THF), 5-methyltetrahydrofolate (5-CH 3 -THF), 5-formyltetrahydrofolate...
ABSTRACT Noroviruses (NoV) annually cause millions of cases gastrointestinal disease in the United States. NoV are associated with raw shellfish outbreaks, particularly oysters, which thought to bioaccumulate particles during filter-feeding process. however, have also been known occur from other common-source food-borne vehicles, such as lettuce, frozen raspberries, and salad. In this study, we evaluated romaine lettuce a potential vehicle for transmission by testing binding distribution...
To determine if human noroviruses (NV) bind to histo-blood group antigens (HBGA) from pig gastric mucosa.An assay was developed measure the inhibition of binding recombinant norovirus-like particles (rNVLP) HBGA in saliva by porcine mucin (PGM). The rNVLP could be inhibited PGM a dose-dependent pattern. Also, captured effectively coated directly on plates and detected polyclonal antibodies against rNVLP. Similarly, saliva, Lewis b d synthetic oligosaccharides (OS), but not an H3 OS or...
Bacterial pathogens and human norovirus (HuNoV) are major cause for acute gastroenteritis caused by contaminated food water. Public waterways can become from a variety of sources flood after heavy rain events, leading to pathogen contamination produce fields. We initiated survey several public watersheds in leafy green production region the Central California Coast determine prevalence HuNoV as well bacterial pathogens. Moore swabs were used collect environmental samples bi-monthly at over...
To develop a sensitive real time immuno-polymerase chain reaction (rtI-PCR) method for detecting norovirus (NV) capsid protein in food samples.The viral antigens were captured by two polyclonal antisera against recombinant Norwalk viral-like particles (rNVLPs). Biotin-conjugated antibodies, avidin and biotin-conjugated DNA reporter used to convert the signals into signals. The was then amplified addition of primers PCR. A PCR order perform quantitative post-PCR analysis. One hundred rNVLPs...
Water is an important route for human norovirus (HuNoV) transmission. Using magnetic beads conjugated with blood group-like antigens (HuNoV receptors), we developed a simple and rapid receptor-binding capture sequestration (RBCMS) method compared it to the existing negatively charged membrane absorption/elution (NCMAE) concentrating HuNoV from sewage effluent. RBCMS required 6-fold-less sample volume than NCMAE also resulted in significantly higher yield of HuNoV. The concentrations...
Human noroviruses (HuNoVs) are the major cause of epidemic nonbacterial gastroenteritis. Although quantitative (real-time) reverse transcription-PCR (qRT-PCR) is widely used for detecting HuNoVs, it only detects presence viral RNA and does not indicate infectivity. blood group antigens (HBGAs) have been identified as receptors/co-receptors both HuNoVs Tulane virus (TV) crucial infection. We propose that infectivity can be evaluated with a molecular assay based on receptor-captured viruses....