A5024183769- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Listeria monocytogenes in Food Safety
- Mycotoxins in Agriculture and Food
- Child Nutrition and Water Access
- Plant Disease Resistance and Genetics
- Enterobacteriaceae and Cronobacter Research
- Brucella: diagnosis, epidemiology, treatment
- Animal Virus Infections Studies
- Biosensors and Analytical Detection
- Bacterial Genetics and Biotechnology
- Mass Spectrometry Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Immunology Research
- Plant Pathogens and Fungal Diseases
- Probiotics and Fermented Foods
Western Regional Research Center
2014-2024
Agricultural Research Service
2014-2024
United States Department of Agriculture
2017-2022
Antimicrobial resistance in Shiga toxin-producing Escherichia coli (STEC) O157 and non-O157 is a matter of increasing concern. The aim the present study was to investigate antimicrobial profiles STEC recovered from feces domestic farm animals agricultural Culiacan Valley Northwestern Mexico. All examined strains showed susceptibility five antimicrobials, ceftazidime, ceftriaxone, ciprofloxacin, nalidixic acid, trimethoprim-sulfamethoxazole. However, four ampicillin, cephalothin,...
Shiga toxin-producing Escherichia coli (STEC) is a zoonotic enteric pathogen that causes human gastrointestinal illnesses. The present study characterized the virulence profiles of O157 and non-O157 STEC strains, recovered from domestic animals in small rural farms within agricultural Culiacan Valley Mexico. Virulence genes coding for adhesins, cytotoxins, proteases, subtypes toxin (Stx), other effectors were identified strains by PCR. genotyping analysis revealed presence nleA, nleB, nleE,...
Bacterial pathogens and human norovirus (HuNoV) are major cause for acute gastroenteritis caused by contaminated food water. Public waterways can become from a variety of sources flood after heavy rain events, leading to pathogen contamination produce fields. We initiated survey several public watersheds in leafy green production region the Central California Coast determine prevalence HuNoV as well bacterial pathogens. Moore swabs were used collect environmental samples bi-monthly at over...
Shiga toxin-producing Escherichia coli (STEC) serotype O145 is one of the major non-O157 serotypes associated with severe human disease. Here we examined genetic diversity, population structure, virulence potential, and antimicrobial resistance profiles environmental strains recovered from a produce production region in California. Multilocus sequence typing analyses revealed that type 78 (ST-78), common ST clinical strains, was predominant genotype among strains. Similarly, all California...
Escherichia coli is a leading cause of human enteric diseases worldwide. The rapid and accurate causal agent identification to particular source represents crucial step in the establishment safety health measures affected populations would thus provide insights into relationship traits that may contribute for pathogen persistence reservoir. objective present study was characterize over two hundred E. strains from different isolation sources Mexico by conducting correspondence analysis...
Salmonella enterica is a leading cause of human gastrointestinal disease worldwide. Given that persistent in aquatic environments, this study examined the prevalence, levels and genotypic diversity isolates recovered from major rivers an important agricultural region northwestern Mexico. During 13-month period, total 143 river water samples were collected subjected to size-exclusion ultrafiltration, followed by enrichment, selective media for isolation quantitation. The next-generation...
The rapid spreading of polymyxin E (colistin) resistance among bacterial strains through the horizontally transmissible mcr-1 and mcr-2 plasmids has become a serious concern. emergence these genes in Shiga toxin-producing Escherichia coli (STEC), group human pathogenic bacteria was even more worrisome, urging us to investigate prevalence mcr STEC isolates. A total 1000 isolates, recovered from livestock, wildlife, produce other environmental sources major production region for leafy...
Human noroviruses (NoV) are the leading cause of human gastroenteritis in populations all ages and linked to most foodborne outbreaks worldwide. Hepatitis A virus (HAV) is another important enteric considered common agent causing acute liver disease In present study, a focused, low-density DNA microarray was developed validated for simultaneous identification foodborne-associated genotypes NoV HAV. By employing novel algorithm, capture probes were designed target variable genomic regions...
ABSTRACT Shiga toxin-producing Escherichia coli is a foodborne and waterborne pathogen responsible for outbreaks of human gastroenteritis. This report documents the draft genome sequences seven O113:H21 strains recovered from livestock, wildlife, soil samples major agricultural region leafy greens in California, USA.
A set of 45 environmental strains Shiga toxin producing Escherichia coli (STEC) from three California counties were analyzed for production by nanospray liquid chromatography-mass spectrometry and Vero cell bioassay. The STEC in this comprised six serotypes ((O113:H21, O121:H19, O157:H7, O6:H34, O177:H25, O185:H7) each containing either the stx2a or stx2c operon. Six seven O113:H21 found to contain two distinct operons. Eight O157:H7 possessed a operon whose subunit gene was interrupted an...
Shiga toxin-producing Escherichia coli (STEC) are zoonotic enteric pathogens linked to human gastroenteritis worldwide. To aid the development of pathogen control efforts, present study characterized genotypic diversity and pathogenic potential STEC recovered from sources near agricultural fields in Northwest Mexico. Samples were collected irrigation river water domestic animal feces farms proximal subjected enrichment followed by immunomagnetic separation plating on selective media for...
Non-typhoidal Salmonella enterica is a leading cause of gastrointestinal illnesses in the United States. Among 2,600 different S . serovars, Infantis has been significantly linked to human and frequently recovered from broilers chicken parts U.S. A key virulence determinant serovar presence megaplasmid pESI, conferring multidrug resistance. To further characterize potential this serovar, present study identified types plasmids harbored by strains, surface waters adjacent leafy greens farms...
Citrobacter werkmanii is an emerging and opportunistic human pathogen found in developing countries a causative agent of wound, urinary tract, blood infections. The present study conducted comparative genomic analyses C. strain collection from diverse geographical locations sources to identify the relevant virulence antimicrobial resistance genes. Pangenome divided examined strains into five distinct clades; subsequent classification identified genes with functional roles carbohydrate...
Shiga toxin-producing Escherichia coli O157:H7 is a foodborne pathogen and responsible for outbreaks of human gastroenteritis. This report documents the draft genome sequences nine cattle strains, which were identified to be PCR positive toxin gene but displayed different levels functional activity.
The bacterial foodborne pathogen Listeria monocytogenes has been implicated in fresh produce outbreaks with a significant economic impact. Given that L. is widespread the environment, food production facilities constantly monitor for presence of species. To develop surveillance platform processing facilities, this study conducted comparative genomic analysis identification conserved high copy sequences ribosomal RNA Simulated folding was performed to assess accessibility identified regions...