Processing the traditional Chinese medicine by fermentation has drawn widely concern. In this study, Kombucha SCOBY was utilized to ferment rose and jujube kernel enhancing its sedative hypnotic effect. Results showed that content of 6-feruloylspinosin spinosin, as typical functional substances exert effects, increased 7% in broth compared with infusion broth. The gallic acid, one antioxidant phenolic rose, 15.9% higher addition, jujuboside B, which profound effects but absent initial...

The demand for natural lactone gamma-decalactone (GDL) has increased in the fields of food and cosmetic products. However, low productivity during bioprocessing limits its industrial production. In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used long-term biotransformation production GDL. effects carrier on related mechanisms were investigated. BC-ALG carriers showed improved mechanical strength over ALG carriers, with their internal...

A number of yeast species can transform ricinoleic acid into γ-decalactone, a high-value compound with fruity aroma, through β-oxidation. This study investigated the effect l-carnitine on γ-decalactone production by Saccharomyces cerevisiae MF013 to increase β-oxidation rate. Results showed that shortened biotransformation period approximately 10 h and increased 19.5%. γ-Caprolactone, γ-octalactone, γ-dodecalactone were separately added medium prevent degradation cells at end...

Human noroviruses (HuNoVs) are the major non-bacterial pathogens causing acute gastroenteritis in people of all ages worldwide. No stable culture system vitro is available for routing detection multiple strains HuNoVs. A simple and rapid method HuNoVs great significance preventing controlling this pathogen. In work, an electrochemical biosensor sensitive fast was constructed based on a screen-printed carbon electrode (SPCE). Gold nanoparticles protein-A were applied SPCE surface enhancement...

Human Noroviruses (HuNoVs) are the main cause of nonbacterial gastroenteritis. Contaminated produce is a vehicle for dissemination HuNoVs. In this study, we used an ice nucleation protein mediated surface display system to present protruding domain GII.4 HuNoV capsid on bacterial and it as new strategy explore interaction between receptor candidates from romaine lettuce. The surface-displayed proteins were confirmed transformed bacteria by immunofluorescence assay. distribution patterns in...

Natural 2-phenylethanol (2-PE) is an important flavoring that emits the aroma of roses. During biotransformation, quality natural 2-PE affected by its main by-products, which include butanol, isobutyric acid, butyric and isovaleric acid. Thus, controlling undesirable by-product formation can reduce effect odor on quality. was produced through biotransformation using l-phenylalanine as a substrate glucose carbon source. Ascorbic acid added to system improve redox reaction suppress generation...

Abstract BACKGROUND The natural gamma‐decalactone (GDL) produced by microbial fermentation is an essential lactone compound as a generally recognized safe food additive. Effective control of the substrate ricinoleic acid (RA) concentration in biotransformation medium bottleneck for industrial manufacture GDL. In this study, porous starch (P‐S) was utilized carrier RA to its distribution medium. effect P‐S on evaluated and mechanism revealed. RESULTS Scanning electron microscopy,...

Lymphocyte activation gene-3 (LAG-3) is a type I transmembrane protein with structural similarities to CD4. Overexpression of LAG-3 enables cancer cells escape immune surveillance, while its blockade reinvigorates exhausted T and strengthens anti-infection immunity. Blockade may have antitumor effects. Here, we generated novel anti-LAG-3 chimeric antibody, 405B8H3(D-E), through hybridoma technology from monoclonal antibodies produced in mice. The heavy-chain variable region the selected...

A new application of oily cold rolling mill sludge for preparing Fe₂O₃/graphene as anodes lithium-ion batteries.

Abstract Applying capillary zone electrophoresis (CZE) to separate the components of Cephradine for Injection: cephradine, and L‐arginine, as well cephalexin, which is degradation product cephradine was studied. The best results were achieved with background electrolyte consisting 50 mM disodium hydrogen phosphate buffer at pH 6.5 an applied voltage 20 kV in a bare fused‐silica capillary. samples injected mbar 4 s. temperature 25°C UV detection performed wavelength 195 nm. Histidine used...

Eplerenone is a drug that protects the cardiovascular system. 11α-Hydroxycanrenone key intermediate in eplerenone synthesis. We found although cytochrome P450 (CYP) enzyme system Aspergillus ochraceus strain MF018 could catalyse conversion of canrenone to 11α-hydroxycanrenone, its biocatalytic efficiency low. To improve 11α-hydroxycanrenone production, CYP monooxygenase-coding gene was predicted and cloned based on whole-genome sequencing results. A recombinant A. MF010 with high expression...

Abstract BACKGROUND 11α‐hydroxyandrosta‐1,4‐diene‐3,17‐dione (11α‐ OH‐ADD ) serves as an important steroid intermediate for many hormone pharmaceuticals. However, the poor aqueous solubility of β‐sitosterol which is substrate hinders its productivity efficiency. This paper reports a new method biotransformation to 11α‐ by mixed cultivation Mycobacterium MF006 and Aspergillus ochraceus MF007 with organic silica hollow spheres ( OSHS improve dispersibility based on excellent adsorption...

Waste rolling oil is a highly emulsified black liquid that difficult to degrade under natural state and has become great environmental problem. In this research, we dopted novel method called one-step flocculation deal with waste oil, identify the best refining conditions separate analyze extracted solid. Using method, recovered almost similar fresh and, therefore, suitable for recycling usage. Solid separated from contained significant amounts of mineral fines could be used as raw material.

We report here the draft genome sequence of Enterobacter sp. strain MF024, a bacterium that can biosynthesize 2-phenylethanol through both Ehrlich pathway and de novo pathway. It has potential use for production 2-phenylethanol.

The distribution function of the current multidimensional data association algorithm does not have timeliness, which causes to generate interference signals during process, and correlation trajectory errors. Based on DNAzyme, a is proposed. classification characteristics mostly distributed adopted extract characteristic parameters data. At same time, internal storage structure different strengthened ensure safe connect signal between sensor in as result, establishes good communication relationship.

Ethyl phenylacetate can be widely applied in many industries, such as food, medicines, cosmetics and medicinal herbs. At the moment, production of natural ethyl is very limited. However, biotransformation has an extensive application prospect. This paper written by taking phenylacetic acid tolerance esterifying enzyme activity two indexes for screening HA14 strain hansenula anomala mutagenic which regarded microorganism through biotransformation. By optimizing condition esterification...

Substrate solubility in steroid biotransformation is critical for enhancing the of hydrophobic compounds. In this study, sulfobutyl ether-β-cyclodextrin (SBE-β-CD) complexation technique was used β-sitosterol to androsta-1, 4-diene-3, 17-dione with Mycobacterium ATCC25795. The production yield increased by 26.72%, and course shortened 24h using β-sitosterol/SBE-β-CD inclusion complexes as substrates (1.0 g/L). Fourier transform infrared spectroscopy differential scanning calorimetry...