A5032241625- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- MicroRNA in disease regulation
- Reproductive System and Pregnancy
- Single-cell and spatial transcriptomics
- Ruminant Nutrition and Digestive Physiology
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- 3D Printing in Biomedical Research
- Exercise and Physiological Responses
- Microgrid Control and Optimization
- Gut microbiota and health
- RNA modifications and cancer
- Hippo pathway signaling and YAP/TAZ
- Ovarian function and disorders
- Birth, Development, and Health
Westlake University
2024-2025
Inner Mongolia University
2020-2024
China Agricultural University
2015-2020
Abstract Spatially ordered embryo-like structures self-assembled from blastocyst-derived stem cells can be generated to mimic embryogenesis in vitro. However, the assembly system and developmental potential of such needs further studied. Here, we devise a nonadherent-suspension-shaking generate (ETX-embryoids) using mouse embryonic, trophoblast extra-embryonic endoderm cells. When cultured together, three cell types aggregate sort into lineage-specific compartments. Signaling among these...
Preimplantation embryos undergo zygotic genome activation and lineage specification resulting in three distinct cell types the late blastocyst. The molecular mechanisms underlying this progress are largely unknown bovines. Here, we sought to analyze an extensive set of regulators at single-cell level define events involved development bovine Using a quantitative microfluidics approach single cells, analyzed mRNA levels 96 genes known function early embryonic maintenance stem pluripotency...
Pigs have emerged as one of the most popular large animal models in biomedical research, which many cases is considered a superior choice over rodent models. In addition, transplantation studies using pig pluripotent stem (PS) cell derivatives may serve testbed for safety and efficacy prior to human trials. Recently, it has been shown that mouse PS cells cultured LCDM (recombinant LIF, CHIR 99021, (S)-(+)-dimethindene maleate, minocycline hydrochloride) medium exhibited extended...
Abstract Pluripotent stem cells have the potential to generate embryo models that can recapitulate developmental processes in vitro. Large animals such as pigs may also benefit from stem-cell-based for improving breeding. Here, we report generation of blastoids porcine embryonic (pESCs). We first develop a culture medium 4FIXY derive pESCs. 3D two-step differentiation strategy The resulting exhibit similar morphology, size, cell lineage composition, and single-cell transcriptome...
Abstract The ability to generate germ cells from pluripotent stem (PSCs) is valuable for human regenerative medicine and animal breeding. Germ cell-like (GCLCs) have been differentiated mouse PSCs, but not porcine which are considered an ideal model cell applications. Here, we developed a defined culture system the induction of primordial (PGCLCs) induced PSCs (piPSCs). identity PGCLCs was characterized by observing morphology, detecting marker gene expression evaluating epigenetic...
The pluripotency of stem cells determines their developmental potential. While the states pluripotent are variable and interconvertible, mechanisms underlying acquisition maintenance remain largely elusive. Here, we identified that methylenetetrahydrofolate dehydrogenase (NAD+-dependent), methenyltetrahydrofolate cyclohydrolase (Mthfd2) plays an essential role in maintaining embryonic cell promoting complete reprogramming induced cells. Mechanistically, mitochondria, Mthfd2 maintains...
Abstract Porcine embryonic stem cells (ESCs) are excellent models for exploring embryogenesis, producing genetically enhanced farm animals, and improving breeding. Various chemicals have been applied to generate porcine ESCs from embryos, which differ mouse human ESC derivation. Wnt inhibitors XAV939 or IWR1 required isolate maintain ESCs. How specify fate decisions remains poorly understood. Additionally, whether can be converted extraembryonic endoderm (XEN) without genetic interventions...
Mutton is one of the most widely consumed meats globally. The Chinese Mongolian sheep (MS) breed an indigenous characterised by high-quality meat and strong adaptability. Dorper × crossbred (DS) improved with a rapid growth rate high mutton yield found in parts China. rumen microbiota known to play key role shaping host nutrition health. However, carcass traits nutritional qualities DS MS remain poorly defined, as does how microbes affect these characteristics. objective this study was...
Cattle have emerged as one of the most important domestic animals widely used for meat, milk, and fur. Derivation bovine pluripotent stem cells (PSCs) can be applied in drug selecting human disease modeling facilitated agriculture‐related applications such production genetically excellent cattle by gene editing. Extended PSCs (EPSCs), capable differentiating into embryonic extraembryonic parts, been generated mouse, human, pig. Whether EPSCs could generated, their chimeric competency remains...
Developmental pluripotency-associated 2 (Dppa2) and developmental 4 (Dppa4) as positive drivers were helpful for transcriptional regulation of zygotic genome activation (ZGA). Here, we systematically assessed the cooperative interplay Dppa2 Dppa4 in regulating cell pluripotency found that simultaneous overexpression Dppa2/4 can make induced pluripotent stem cells closer to embryonic (ESCs). Compared with other transcription factors, regulate majorities signaling pathways by binding on...
Developmental pluripotency-associated 2 (DPPA2) and DPPA4 are crucial transcription factors involved in maintaining pluripotency humans mice. However, the role of DPPA2/4 bovine extended pluripotent stem cells (bEPSCs) has not been investigated. In this study, a subset bEPSC-related differentially expressed genes (DEGs), including DPPA2 DPPA4, was identified based on multiomics data (ATAC-seq RNA-seq). Subsequent investigations revealed that double overexpression facilitates reprogramming...
After zygotic genome activation and lineage specification, zygotes develop into late blastocysts comprising three distinct cell types. The molecular mechanisms underlying this progress are largely unknown in pigs. Here, we intended to analyze an extensive set of regulators at the single-cell level define events involved development porcine blastocysts. Using a quantitative microfluidics approach single cells, detected mRNA levels 96 genes known function early embryonic maintenance stem...
Abstract Induced pluripotent stem cells (iPSCs) have variable expression levels of a series genes that affect their potential, but the regulatory mechanisms controlling reprogramming remain unclear. By testing efficiency iPSC generation using Oct4, Sox2, Klf4 (termed OSK) plus one additional gene, we found Rab32 improved efficiency. We established system for detecting number and size lipid droplets autophagosomes per cell tracking morphological changes during reprogramming. Our results...
It is not fully clear why there a higher contribution of pluripotent stem cells (PSCs) to the chimera produced by injection PSCs into 4-cell or 8-cell stage embryos compared with blastocyst injection. Here, we show that only embryonic (ESCs) but also induced (iPSCs) can generate F0 nearly 100% donor cell-derived mice embryo injection, and approach has "dose effect". Through an analysis PSC-secreted proteins, Activin A was found impede epiblast (EPI) lineage development while promoting...
Human embryonic stem cells (hESCs) possess the ability to differentiate into insulin-producing (IPCs), which can be used treat type I diabetes. miR-375 is an essential transcriptional regulator in development and maturation of pancreas. In this study, we optimized a protocol hESCs IPCs successfully obtained IPCs. Then, performed overexpression inhibition experiments on at different stages differentiation RNA-seq. The results showed that expression fluctuated during hESC was affected by...
The Arbas cashmere goat is a unique biological resource that plays vital role in livestock husbandry China. LCDM medium with special small molecules (consisting of human LIF, CHIR99021, (S)-(+)-dimethindene maleate, and minocycline hydrochloride) for generation pluripotent stem cells (PSCs) bidirectional developmental potential mice, humans, pigs, bovines. However, there no report on whether can support PSCs the same ability goats. In this study, we applied to generate induced (giPSCs) from...
Skeletal muscle is a critical tissue for physiological activity and energy utilization, its insulin resistance indispensable the development of Type 2 diabetes. Dietary intervention has become one efficient methods enhancing metabolic health skeletal muscle. Plant extracts from fruits, tea, vegetables, Chinese herbal medicine, have been demonstrated to enhance myogenesis, reduce atrophy, improve oxidative functions. In this review, current knowledge about plant their application in...
Our study examined the in vivo chimeric and survival capacities of chimeras created by injecting tetraploid embryonic stem cells (ESCs) expressing green fluorescent protein (GFP) into diploid embryos. At 3.5 days post-coitum (dpc) 4.5 dpc, ESCs were able to contribute inner cell mass (ICM) just as tagged with GFP. 6.5 8.0 dpc 10.5 manifested same location ESCs. The GFP extraembryonic tissues fetuses ESC determined fluorescence activated sorting (FACS). Furthermore, contributed development...
The Arbas cashmere goat is a unique biological resource that plays vital role in livestock husbandry China. LCDM medium with special small molecules (consisting of human LIF, CHIR99021, (S)-(+)-dimethindene maleate, and minocycline hydrochloride) for generation pluripotent stem cells (PSCs) bidirectional developmental potential mice, humans, pigs, bovines. However, there no report on whether can support PSCs the same ability goats. In this study, we applied to induced (giPSCs) from fetal...
Pancreatic adenocarcinoma (PAAD) is a pancreatic disease with considerable mortality worldwide. Because of lack obvious symptoms at the early stage, most PAAD patients are diagnosed terminal stage and prognosis usually poor. In this study, we firstly obtained RNA sequencing data 181 from The Cancer Genome Atlas (TCGA) database to identify diagnostic biomarkers for PAAD. Survival-related mRNAs were identified using weighted gene co-expression network analysis (WGCNA), then linear prognostic...