A5016361904- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Enzyme Structure and Function
- Mass Spectrometry Techniques and Applications
- Plant Stress Responses and Tolerance
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Protein Structure and Dynamics
- Photosynthetic Processes and Mechanisms
- Enzyme Catalysis and Immobilization
- Genomics and Chromatin Dynamics
- Chemical Synthesis and Analysis
- RNA Research and Splicing
- Amino Acid Enzymes and Metabolism
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Genetics and Neurodevelopmental Disorders
- Viral Infections and Vectors
- Protein Degradation and Inhibitors
- Advanced Proteomics Techniques and Applications
- Plant nutrient uptake and metabolism
China Agricultural University
2015-2024
Hong Kong Biotechnology Organization
2020
Qilu University of Technology
2018
Shandong Academy of Sciences
2018
John Wiley & Sons (Germany)
2018
Shenzhen Institutes of Advanced Technology
2017
University of Chinese Academy of Sciences
2017
First Affiliated Hospital of Nanchang University
2017
Nanchang University
2017
University of Minnesota
2010-2015
To investigate the regulation of LaCl3 on lipopolysaccharides (LPS)-induced pro-inflammatory cytokines and adhesion molecules in human umbilical vein endothelial cells (HUVECs).Primary cultured HUVECs were pretreated with 2.5 µM for 30 min followed by 1 µg/ml LPS 2 h. Pro-inflammatory cytokine molecule expressions determined real-time RT-PCR ELISA. NF-κB/p65 nuclear translocation was examined immunofluorescence immuno-blot, its DNA-binding activity measured chemiluminescence. Recruitment...
Abstract Catalases are key regulators of reactive oxygen species homeostasis in plant cells. However, the regulation catalase activity is not well understood. In this study, we isolated an Arabidopsis thaliana mutant, no activity1-3 (nca1-3) that hypersensitive to many abiotic stress treatments. The mutated gene was identified by map-based cloning as NCA1, which encodes a protein containing N-terminal RING-finger domain and C-terminal tetratricopeptide repeat-like helical domain. NCA1...
Abstract Transient ischemia is a leading cause of cognitive dysfunction. Postischemic ROS generation and an increase in the cytosolic Zn 2+ level ([Zn ] c ) are critical delayed CA1 pyramidal neuronal death, but underlying mechanisms not fully understood. Here we investigated role ROS-sensitive TRPM2 (transient receptor potential melastatin-related 2) channel. Using vivo vitro models ischemia–reperfusion, showed that genetic knockout strongly prohibited [Zn , generation, death postischemic...
Abstract Ferric uptake regulator (Fur) plays a key role in the iron homeostasis of prokaryotes, such as bacterial pathogens, but molecular mechanisms and structural basis Fur–DNA binding remain incompletely understood. Here, we report high-resolution structures Magnetospirillum gryphiswaldense MSR-1 Fur four different states: apo-Fur, holo-Fur, Fur– feoAB1 operator complex Pseudomonas aeruginosa box complex. Apo-Fur is transition metal ion-independent dimer whose induces profound...
The newly emerging variants of SARS-CoV-2 from South Africa (B.1.351/501Y.V2) and Brazil (P.1/501Y.V3) have led to a higher infection rate reinfection COVID-19 patients. We found that the mutations K417N, E484K, N501Y within receptor-binding domains (RBDs) virus could confer ~2-fold binding affinity human receptor, angiotensin converting enzyme 2 (ACE2), compared wildtype RBD. mutated version RBD also completely abolishes bamlanivimab, therapeutic antibody, in vitro. Detailed analysis shows...
The Jumonji C domain is a catalytic motif that mediates histone lysine demethylation. C-containing oxygenase JMJD2A specifically demethylates tri- and dimethylated lysine-9 lysine-36 of 3 (H3K9/36me3/2). Here we present structures the core complexed with methylated H3K36 peptide substrates in presence Fe(II) N -oxalylglycine. We found interaction between peptides largely involves main chains enzyme peptide. peptide-binding specificity primarily determined by primary structure peptide, which...
Abstract Respiratory infections, including Mycoplasma pneumoniae (Mp), contribute to asthma pathobiology. To date, the mechanisms underlying increased susceptibility of asthmatics airway Mp infection remain unclear. Short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein is a recently described large epithelial cell-derived molecule that was predicted exert host defense activities. However, SPLUNC1 function regulation in an infectious or allergic milieu are still unknown. We...
The major chemical constituents and antioxidant activities of water-soluble polysaccharide (PS) fractions isolated from three edible mushrooms were investigated. Crude PS (CPS) the mushroom hot water extract by ethanol precipitation contained 15–30% (w/w) total protein 2–5% phenolics. CPS fractionated ion exchange chromatography neutral, slightly ionic (eluted with 0 0.1 M NaCl) 70–80% carbohydrate low phenolic contents, while 0.3 0.7 had higher contents (20–70%) phenolics (2–13%). all...
The central region of MDM2 is critical for p53 activation and tumor suppression. Upon ribosomal stress, this bound by proteins, particularly protein L11 (RPL11), leading to inactivation subsequent activation. Here, we solved the complex structure human MDM2–RPL11 at 2.4 Å. extensively interacts with RPL11 through an acidic domain two zinc fingers. Formation induces substantial conformational changes in both proteins. RPL11, unable bind mutants, fails induce cells. mimics 28S rRNA binding...
Significance Enzymes responsible for the clipping of histone tails and removal arginine-methylated still remain elusive. The underlying mechanism high turnover rate in nonproliferated cells is a mystery. How RNA polymerase II overcomes nucleosome barriers during transcription unknown. This article described discovery JmjC domain containing subfamily members JMJD5 JMJD7, which could be these unsolved puzzles epigenetics fields.
Abstract All positive-stranded RNA viruses with genomes >∼7 kb encode helicases, which generally are poorly characterized. The core of the nidovirus superfamily 1 helicase (HEL1) is associated a unique N-terminal zinc-binding domain (ZBD) that was previously implicated in regulation, genome replication and subgenomic mRNA synthesis. high-resolution structure arterivirus (nsp10), alone complex polynucleotide substrate, now provides first insights into structural basis for function. A...