A5101746668- RNA modifications and cancer
- Prion Diseases and Protein Misfolding
- RNA Interference and Gene Delivery
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Meat and Animal Product Quality
- Advanced Battery Technologies Research
- Advancements in Battery Materials
- Identification and Quantification in Food
- RNA Research and Splicing
- Advanced Battery Materials and Technologies
- RNA regulation and disease
- Chromosomal and Genetic Variations
- Listeria monocytogenes in Food Safety
- Plant Gene Expression Analysis
- Microbial Metabolism and Applications
- Metabolism and Genetic Disorders
- Microbial Inactivation Methods
- Microfluidic and Bio-sensing Technologies
- DNA and Nucleic Acid Chemistry
- Constructed Wetlands for Wastewater Treatment
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Microfluidic and Capillary Electrophoresis Applications
China Agricultural University
2016-2023
Aarhus University
2019
University of Minnesota
2010
Karolinska Institutet
2006
University at Albany, State University of New York
1997-2005
New York State Department of Health
1997-2005
Wadsworth Center
1997-2005
Abbott (United States)
2000
Rensselaer Polytechnic Institute
1997
National Cancer Institute
1997
Floral organs in rice (Oryza sativa) can be purple, brown, or red color due to the accumulation of flavonoids, but molecular mechanism underlying specific organ pigmentation is not clear. Here, we propose a C–S–A gene model for hull and characterize it through genetic, molecular, metabolomic approaches. Furthermore, conducted phylogenetic studies reveal evolution color. In this system, C1 encodes R2R3-MYB transcription factor acts as color-producing gene, S1 bHLH protein that functions...
Inteins are protein-splicing elements, most of which contain conserved sequence blocks that define a family homing endonucleases. Like group I introns encode such endonucleases, inteins mobile genetic elements. Recent crystallography and computer modeling studies suggest consist two structural domains correspond to the endonuclease To determine whether bipartite structure is mirrored by functional independence domain, entire component was deleted from Mycobacterium tuberculosis recA intein....
Recently, the NLRP3 inflammasome activation in eyes has been known to be associated with pathogenesis of age-related macular degeneration. The aim this study was investigate protective effects cyanidin-3-glucoside (C3G), an important anthocyanin great potential for preventing eye diseases, against 4-hydroxyhexenal- (HHE-) induced inflammatory damages human retinal pigment epithelial cells, ARPE-19. We noticed that C3G pretreatment ARPE-19 cells rescued HHE-induced antiproliferative effects....
Small nucleolar RNAs (snoRNAs) and Cajal body-specific (scaRNAs) are named for their subcellular localization within nucleoli bodies (conserved subnuclear organelles present in the nucleoplasm), respectively. They have been found to play important roles rRNA, tRNA, snRNAs, even mRNA modification processing. All snoRNAs fall two categories, box C/D H/ACA snoRNAs, according distinct sequence secondary structure features. Box mainly function guiding 2'-O-ribose methylation pseudouridilation,...
Mitochondrial malfunction is a universal and critical step in the pathogenesis of many neurodegenerative diseases including prion diseases. Dynamin-like protein 1 (DLP1) one key regulators mitochondrial fission. In this study, we investigated role DLP1 fragmentation dysfunction neurons using vitro vivo disease models. Mitochondria became fragmented redistributed from axons to soma, correlated with increased expression murine primary (N2a cells) treated peptide PrP106-126 as well...
Mass spectrometry (MS) has become essential for efficient and accurate quantification of proteins proteomes and, thus, a key technology throughout all biosciences. However, validated MS methods are still scarce meat quality research applications. The objective this work was to develop compare two targeted proteomic methods, namely, selected reaction monitoring (SRM) sequential window acquisition theoretical spectra (SWATH), the 11 bovine muscle that may be indicators color. Both require...
Abstract Relative of Early Flowing 6 (REF6) is a DNA-sequence-specific H3K27me3/2 demethylase that contains four zinc finger (ZnF) domains and targets several thousand genes in Arabidopsis thaliana . The ZnF are essential for binding target genes, but the structural basis remains unclear. Here, we determined crystal structures REF6-DNA complex, revealing unique REF6-family-specific half-cross-braced (RCZ) domain two C2H2-type ZnFs. DNA-binding induces profound conformational change hinge...
Prion disease represents a group of fatal neurogenerative diseases in humans and animals that are associated with energy loss, axonal degeneration, mitochondrial dysfunction. Axonal degeneration is an early hallmark neurodegeneration triggered by SARM1. We found depletion or dysfunctional mutation SARM1 protected against NAD+ functional disorder induced the neurotoxic peptide PrP106-126. supplementation rescued prion-triggered dysfunction overexpression suppressed this protective effect....
Adenine N6 methylation in DNA (6mA) is a well-known epigenetic modification bacteria, phages, and eukaryotes. Recent research has identified the Mpr1/Pad1 N-terminal (MPN) domain-containing protein (MPND) as sensor that may recognize 6mA However, structural details of MPND molecular mechanism their interaction remain unknown. Herein, we report first crystal structures apo-MPND MPND-DNA complex at resolutions 2.06 Å 2.47 Å, respectively. In solution, assemblies both are dynamic. addition, was...
Abstract Testicular nuclear receptor 4 (TR4) modulates the transcriptional activation of genes and plays important roles in many diseases. The regulation TR4 on target involves direct interactions with DNA molecules via DNA-binding domain (DBD) recruitment coregulators by ligand-binding (LBD). However, their regulatory mechanisms are unclear. Here, we report high-resolution crystal structures TR4DBD, TR4DBD–DNA complexes TR4LBD–JAZF1 complex. For recognition, multiple factors come into play,...
Single-step fusion-based affinity purification of proteins with pH-controllable linkers was carried out in a fluidic device. The were previously derived from self-splicing protein elements called inteins. Two different generated to solve two distinct separation problems: one for rapid single-step wide range proteins, and the other specifically cytotoxic proteins. Scale-down factors 185 resulted separations 27 μl bed-volume. A rotating CD format chosen because its simplicity effecting fluid...
Lung Adenocarcinoma (LUAD) is one of the leading causes to cancer-related deaths worldwide and exhibited extensive molecular diversity in its cellular components. In summary, this study examined expression function KARS various LUAD cell populations using single-cell RNA sequencing (scRNA-seq) identify prognostic value as well potential implications for therapy. All datasets related were analyzed previous published studies, which was downloaded from The Cancer Genome Atlas (TCGA),...