A5014951086- Monoclonal and Polyclonal Antibodies Research
- Theoretical and Computational Physics
- RNA and protein synthesis mechanisms
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Stochastic processes and statistical mechanics
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Multimedia Communication and Technology
- Respiratory Support and Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Telecommunications and Broadcasting Technologies
- Quantum many-body systems
- Material Dynamics and Properties
- Receptor Mechanisms and Signaling
- Image and Video Quality Assessment
- RNA Research and Splicing
- Video Coding and Compression Technologies
- Transgenic Plants and Applications
- Neonatal Respiratory Health Research
- Complex Systems and Time Series Analysis
- bioluminescence and chemiluminescence research
- Bacteriophages and microbial interactions
- Asthma and respiratory diseases
- Physics of Superconductivity and Magnetism
The Ohio State University
2016-2025
QED Labs
1988-2020
Academy of Motion Picture Arts and Sciences
2019
Imperial College London
2014-2018
European Broadcasting Union
1991-2014
St. Mary's Hospital
2014
St Mary's Hospital
2014
Imperial College Healthcare NHS Trust
2014
St Mary's Hospital
2014
Ross Laboratories (United States)
2014
A clinical scoring system has been developed to detect impending or existing respiratory failure in childhood status asthmaticus. The score is based on a numerical evaluation of oxygenation, gas exchange, work breathing, airway obstruction, and cerebral function. highly significant correlation exists between the arterial carbon dioxide tension (Pco 2 ). oxygen (Po ) during air breathing also appear be significantly related.
The method of developing exact power-series expansions for the partition function ZN and related thermodynamic functions Ising model valid below critical point is generalized to include exchange interactions between first-, second-, third-neighbor pairs. Expansions spontaneous magnetization M0(T) zero field susceptibility χ0(T) are derived through sixth order perturbation s.q. lattice, fifth Δ′r, b.c.c., s.c., f.c.c. lattices, when J1SizSjz J2SkzSlz present first- second-neighbor spins,...
Two different techniques were used to photochemically modify 50 kDa poly(ether sulfone) (PES) membranes with the monomer N-vinyl-2-pyrrolidinone (NVP) increase surface wettability and decrease adsorptive fouling during constant volume diafiltration of 0.1 wt % bovine serum albumin (BSA). The filtration performance modified was compared that a commercially available PES membrane regenerated cellulose membrane. Both dip immersion modification produced essentially same as cellulose, 30% over...
Abstract This work combines two well‐established technologies to generate a breakthrough in protein production and purification. The first is the of polyhydroxybutyrate (PHB) granules engineered strains Escherichia coli . second recently developed group self‐cleaving affinity tags based on splicing elements known as inteins. By combining these with PHB‐specific binding protein, self‐contained expression purification system has been developed. In this system, PHB‐binding effectively acts an...
Inteins are protein-splicing elements, most of which contain conserved sequence blocks that define a family homing endonucleases. Like group I introns encode such endonucleases, inteins mobile genetic elements. Recent crystallography and computer modeling studies suggest consist two structural domains correspond to the endonuclease To determine whether bipartite structure is mirrored by functional independence domain, entire component was deleted from Mycobacterium tuberculosis recA intein....
The International Telecommunication Union Radiocommunication Sector (ITU-R) published Recommendation (Rec.) BT.2020? Parameter Values for Ultra-High-Definition Television Systems Production and Programme Exchange? [1] in August 2012. parameters television (TV) systems are roughly equivalent to the image format, including pixel count, frame frequency, colorimetry. These basic system determine what kind of visual experiences can provide viewers terms possible field view (FOV) size, quality...
The system described by the Hamiltonian shown is investigated on basis of exact high-temperature series expansions and Green function technique. Expansions for zero-field (B = 0) free energy susceptibility (valid arbitrary lattice structure) are developed to fifth order in reciprocal temperature S ½ with nearest-neighbour forces. critical behaviour discussed two three dimensions using random phase approximation Padé approximants. In particular variation η accurately determined common two-...
Here we introduce a Rapid Adaptable Portable In vitro Detection biosensor platform (RAPID) for detecting ligands that interact with nuclear hormone receptors (NHRs). The RAPID can be adapted field use, allowing rapid evaluation of endocrine disrupting chemicals (EDCs) presence or absence in environmental samples, and also applied drug screening. is based on an engineered, allosterically activated fusion protein, which contains the ligand binding domain from target NHR (human thyroid receptor...
Inteins are the protein analogs of self-splicing RNA introns, as they post-translationally excise themselves from a variety hosts. Intein insertion abolishes, in general, activity its host protein, which is subsequently restored upon intein excision. These elements therefore have potential to be used general molecular "switches" for control arbitrary target proteins. Based on rational design, an intein-based switch has been constructed whose splicing conditionally triggered vivo by presence...
An Ising model is defined on a simple quadratic lattice which has the usual nearest neighbour pair potential terms (-J2 sigma k l) and also triplet (-J3 j coupling spins over elementary triangular mesh cycles formed by connecting half of next sites, such that when J2=0 reduces to with only potentials present. It shown well known self dual property for case J2 not=0, J3=0 holds J2=0, J3 thereby yielding likely result transition point both these cases given exp(-2J2,3/kT)= square root 2-1.
Recombinant proteins have wide applications in the development of pharmaceutical compounds, industrial enzymes, and basic proteomics research. In this way, efficient production recombinant with high purity needs purification methods. Various strategies been devised to improve these such as affinity physicochemical methods which has some advantages over others. Affinity especially fusion indispensable tools for massive parallel production, identification from host systems. These facilitate...
Inteins (intervening proteins) interrupt host proteins and are removed through a protein splicing reaction that ligates adjacent N- C-exteins. The ability of inteins to specifically rearrange peptide bonds has proven exceptionally useful in engineering, thus, methods control intein activity considerable interest. One particularly application is for the removal an affinity tag following purification target N-terminal cleavage (NTC). Typically, extended incubation at high temperature (greater...
To reduce the number of recovery steps during downstream processing and to overcome limitations present fusion-based affinity separations, a controllable self-splicing protein element in form mini-intein was used optimize proteins for both batch flow purification strategies. The ability recover purified demonstrated using tripartite fusion consisting maltose binding domain, truncated intein as linker molecule, interest. characterize expression level, solubility, cleavage rates, pH...