A5048221077- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Protein purification and stability
- Enzyme Catalysis and Immobilization
- Cancer Research and Treatments
- Virus-based gene therapy research
- Chemical Synthesis and Analysis
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- Click Chemistry and Applications
- Advanced Biosensing Techniques and Applications
- Biotin and Related Studies
- bioluminescence and chemiluminescence research
- CAR-T cell therapy research
- Viral gastroenteritis research and epidemiology
- Biosensors and Analytical Detection
- Spaceflight effects on biology
- Viral Infections and Immunology Research
- Tissue Engineering and Regenerative Medicine
Brigham Young University
2016-2025
Provo College
2017
Stanford University
2007-2011
The tyrosine analog p-propargyloxyphenylalanine (pPa), like tyrosine, has limited water solubility. It been postulated that this solubility contributed to reduced cellular uptake of pPa and thus in vivo incorporation into proteins. Using a cell-free protein synthesis system (CFPS) circumvent uptake, incorporated site-specifically proteins with high specificity at yields up 27 times greater than the highest previously reported yield. alkyne group present on provides reactive residue for...
Virus-like particles (VLP) have received considerable attention for vaccine, drug delivery, gene therapy and material science applications. Although the number of unique VLP their applications are rapidly growing, positive impact is limited by current diverse, expensive, typically low-yielding production technologies available. These technologies, when scaled, often result in structurally compositionally inconsistent products. We present Escherichia coli-based cell-free protein synthesis as...
Biotherapeutics have many promising applications, such as anti-cancer treatments, immune suppression, and vaccines. However, due to their biological nature, some biotherapeutics can be challenging rapidly express screen for activity through traditional recombinant methods. For example, difficult-to-express proteins may cytotoxic or form inclusion bodies during expression, increasing the time, labor, difficulty of purification downstream characterization. One potential pathway simplify...
Escherichia coli-based cell extract is a vital component of inexpensive and high-yielding cell-free protein synthesis reactions. However, effective preparation E. coli limited to high-pressure (French press-style or impinge-style) bead mill homogenizers, which all require significant capital investment. Here we report the viability prepared using equipment that both common biotechnology laboratories able process small volume samples. Specifically, assessed low-capital-cost lysis techniques...
Cell-free protein synthesis (CFPS) is a versatile tool for rapid recombinant production and engineering. One drawback of cell-free technology the necessity to store major components—cell extracts energy systems—below freezing in bulky aqueous solutions. Here we describe simple methods lyophilizing preparing powdered systems CFPS. These techniques allow high-density storage that are more robust against temperature bacterial degradation. Our have potential decrease expenses, longer shelf-life...
Background/Objectives: Cancer is a leading cause of death. However, recently developed immunotherapies have shown significant promise to improve cancer treatment outcomes and survival rates. Pembrolizumab, immunotherapy drug, enables strong T-cell response specifically targeting cells patient in more than 16 types cancer. The increasing demand for pembrolizumab, the highest selling drug 2023, increases global dependence on production, which can be vulnerable supply chain disruptions....
Here we introduce a Rapid Adaptable Portable In vitro Detection biosensor platform (RAPID) for detecting ligands that interact with nuclear hormone receptors (NHRs). The RAPID can be adapted field use, allowing rapid evaluation of endocrine disrupting chemicals (EDCs) presence or absence in environmental samples, and also applied drug screening. is based on an engineered, allosterically activated fusion protein, which contains the ligand binding domain from target NHR (human thyroid receptor...
The COVID-19 pandemic has illustrated the global demand for rapid, low-cost, widely distributable and point-of-care nucleic acid diagnostic technologies. Such technologies could help disrupt transmission, sustain economies preserve health lives during widespread infection. In contrast, conventional procedures require trained personnel, complex laboratories, expensive equipment, protracted processing times. this work, lyophilized cell-free protein synthesis (CFPS) toehold switch...
Approximately one third of protein therapeutics are produced in Escherichia coli, targeting a wide variety diseases. However, due to immune recognition endotoxin (a lipid component the E. coli cell membrane), these products must be extensively purified before application avoid adverse reactions such as septic shock. coli-based cell-free synthesis (CFPS), which has emerged promising platform for development and production enhanced therapeutics, provides unique opportunity remove endotoxins...
Combining patient-specific cells with the appropriate scaffold to create functional kidneys is a promising technology provide immunocompatible for 100 000+ patients on organ waiting list. For proper recellularization occur, must possess critical microstructure and an intact vascular network. Detergent perfusion through vasculature of kidney preferred method decellularization; however, harsh detergents could be damaging renal tissue may undesirably solubilize endogenous growth signaling...
Enzymes play an essential role in many different industries; however, their operating conditions are limited due to the loss of enzyme activity presence proteases and at temperatures significantly above physiological conditions. One way improve stability these enzymes against high is encapsulate them protective shells or virus-like particles. This work presents a streamlined, three-step, cell-free protein synthesis (CFPS) procedure that enables rapid vitro production, targeted encapsulation...