A5035907417- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Supercapacitor Materials and Fabrication
- biodegradable polymer synthesis and properties
- Microbial Metabolism and Applications
- Electrospun Nanofibers in Biomedical Applications
- Transgenic Plants and Applications
- Nanofabrication and Lithography Techniques
- Conducting polymers and applications
- Surface Modification and Superhydrophobicity
- Electrochemical Analysis and Applications
- Immune Response and Inflammation
- Electrochemical sensors and biosensors
- Sepsis Diagnosis and Treatment
- Optical Coatings and Gratings
- RNA and protein synthesis mechanisms
- Intensive Care Unit Cognitive Disorders
The Ohio State University
2017-2019
The University of Texas Southwestern Medical Center
2019
Abstract Sub-wavelength antireflection moth-eye structures were fabricated with Nickel mold using Roll-to-Plate (R2P) ultraviolet nanoimprint lithography (UV-NIL) on transparent polycarbonate (PC) substrates. Samples well replicated patterns established an average reflection of 1.21% in the visible light range, 380 to 760 nm, at normal incidence. An excellent property a wide range incidence angles was shown below 4% 50°. Compared unpatterned ultraviolet-curable resin coating, resulting...
The use of cell-free systems to produce recombinant proteins has grown rapidly over the past decade. In particular, protein synthesis (CFPS) based on mammalian cells provide alternative methods for production many proteins, including those that contain disulfide bonds, glycosylation, and complex structures such as monoclonal antibodies. present study, we show robust turbo green fluorescent (tGFP) streptokinase in a system using instrumented mini-bioreactors highly reproducible production. We...
Sepsis is a major cause of patient mortality and morbidity from bacterial infections.Although neutrophils are known to be important in the development sepsis, how distinctive neutrophil subtypes regulate inflammatory processes involved septicemia remains unclear.Preconditioning protects organisms against subsequent higher-dose exposures same, or even different, stimuli.Several studies have reported various effects preconditioning on immune cells.However, detailed mechanisms underlying...
In this work, we describe a novel self-cleaving tag technology based on highly modified split-intein cleaving element. system, the N-terminal segment of an engineered split intein is expressed in E. coli and covalently immobilized onto capture resin, while smaller C-terminal fused to N-terminus desired target protein. The tagged can then be appropriate expression without concern for premature cleaving. During purification, strong association between segments effectively captures resin...
Stroke is the leading cause of death with over 5 million deaths worldwide each year. About 80% strokes are ischemic caused by blood clots. Tissue plasminogen activator (tPa) only FDA-approved drug to treat stroke a wholesale price $6000. tPa now off patent although no biosimilar has been developed. The production complicated 17 disulfide bonds that exist in correctly folded tPA. Here, we present an Escherichia coli-based cell-free protein synthesis platform for expression and report...