A5063456785- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Transgenic Plants and Applications
- Hematopoietic Stem Cell Transplantation
- Biomedical Ethics and Regulation
- PARP inhibition in cancer therapy
- Microbial Metabolic Engineering and Bioproduction
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Gene Regulatory Network Analysis
- CAR-T cell therapy research
- Evolutionary Algorithms and Applications
- Microwave-Assisted Synthesis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Pancreatic function and diabetes
- Innovative Microfluidic and Catalytic Techniques Innovation
University of Maryland, Baltimore County
2018-2023
Cell-Free Protein Synthesis (CFPS) offers many advantages for the production of recombinant therapeutic proteins using CHO cell-free system. However, complex are still difficult to express this method. To investigate current bottlenecks in glycoprotein production, we chose erythropoietin (40% glycosylated), an essential endogenous hormone which stimulates development red blood cells. Here, report (EPO) Using method, EPO was expressed and purified with a twofold increase yield when reaction...
This study describes the cell-free biomanufacturing of a broad-spectrum antiviral protein, griffithsin (GRFT) such that it can be produced in microgram quantities with consistent purity and potency less than 24 h. We demonstrate GRFT production using two independent systems, one plant microbial. Griffithsin quality were verified standard regulatory metrics. Efficacy was demonstrated vitro against SARS-CoV-2 HIV-1 nearly identical to expressed vivo. The proposed process is efficient readily...
Protein therapeutics, also known as biologics, are currently manufactured at centralized facilities according to rigorous protocols. The manufacturing process takes months and the delivery of biological products needs a cold chain. This makes it less responsive rapid changes in demand. Here, we report on technology application for on-demand biologics (Bio-MOD) that can produce safe effective from cell-free systems point care without current challenges long-term storage cold-chain delivery....
Abstract There is an increasing interest in low-cost, facile and versatile thermoplastic bonding for microfluidic applications that can be easily transitioned from laboratory prototyping to industrial manufacturing. In addition, owing the surge usage of microfluidics its adverse effect on environment, it prudent source alternative materials are biodegradable, providing a sustainable, green approach. To address problems, here we introduce environment friendly, low-cost safe welding technology...
This study describes the cell-free biomanufacturing of a broad-spectrum antiviral protein, griffithsin (GRFT) such that it can be produced with consistent purity and potency in less than 24 hours. We demonstrate GRFT production using two independent systems, one plant microbial. Griffithsin quality were verified standard regulatory metrics. Efficacy was demonstrated vitro against SARS-CoV-2 HIV-1 nearly identical to expressed vivo . The proposed process is efficient readily scaled up...