Abstract A hemocytometer‐based trypan blue dye exclusion cell quantitation and viability assay was compared with a similar using simultaneous fluorometric staining fluorescein diacetate propidium iodide. Viable nonviable densities were measured, culture calculated both during the normal growth cycle of murine hybridoma in response to application millimolar concentrations either tert ‐butyl hydroperoxide or ferrous iron. During early phase rapid growth, assay‐based differences viable density...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTModification of DNA bases in mammalian chromatin by radiation-generated free radicalsEwa Gajewski, Govind Rao, Zeena Nackerdien, and Miral DizdarogluCite this: Biochemistry 1990, 29, 34, 7876–7882Publication Date (Print):August 28, 1990Publication History Published online1 May 2002Published inissue 28 August 1990https://pubs.acs.org/doi/10.1021/bi00486a014https://doi.org/10.1021/bi00486a014research-articleACS PublicationsRequest reuse...

Abstract Acid mine drainage (AMD) containing high concentrations of sulfate and heavy metal ions can be treated by biological reduction. It has been reported that the effect metals on sulfate‐reducing bacteria (SRB) stimulatory at lower toxic/inhibitory higher concentrations. The quantification dissolved is critical for design operation an effective AMD bioremediation process. Serum bottle batch reactor studies toxicity to SRB indicate insoluble sulfides inhibit activity as well. mechanism...

Abstract A mathematical model has been developed to simulate the Texaco downflow entrained‐bed pilot‐plant gasifier using coal liquefaction residues and coal‐water slurries as feedstocks. This describes physical chemical processes occurring in an entrained gasifier. The gasification kinetics different complex reactions hydrodynamics mass, momentum energy balances for solid gas phases. Temperature, concentration velocity profiles along reactor height were obtained by solving balances....

Synthesis of an operon fusion protein was investigated in batch and fed-batch cultures at high cell densities recombinant Escherichia coli JM105 [pBAD-GFP::CAT]. Glucose-limited growth achieved without accumulation inhibitory byproducts allowing (110 g L−1 DCW) to be attained. This believed the highest reported value for dry mass E. strain expressing two proteins. Transcription reporter genes, green fluorescent (GFP) chloramphenicol acetyltransferase (CAT), under control pBAD promoter araBAD...

Abstract The adverse effects of copper and zinc on an acetate‐utilizing mixed cultures sulfate‐reducing bacteria (SRB) at concentrations below the toxic concentration (minimum metal which no sulfate reduction is observed) are reported in this paper. Mathematical models were developed to incorporate inhibitory (defined as bacterial population upon exposure decrease metabolic rate by SRB, respectively) into sulfate‐reduction biokinetics. characteristic toxicity inhibition constants obtained...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSorption of toxic organic compounds on wastewater solids: correlation with fundamental propertiesRichard A. Dobbs, Leping Wang, and Rakesh GovindCite this: Environ. Sci. Technol. 1989, 23, 9, 1092–1097Publication Date (Print):September 1, 1989Publication History Published online1 May 2002Published inissue 1 September 1989https://pubs.acs.org/doi/10.1021/es00067a004https://doi.org/10.1021/es00067a004research-articleACS PublicationsRequest reuse...

Three-month-old C3H female mice were given injections of 5-mg pieces mammary adenocarcinoma and then fed diets that either fat free or contained saturated (15% hydrogenated cottonseed oil) linoleate (1–15% corn oil). After 6 weeks, the tumors in diet weighed 3–4 times more than those fat-free saturated-fat diets. Despite a linoleate-free diet, appreciable amounts arachidonate (≈2 9% total fatty acids, respectively). When level dietary oil was increased from 1 to 15%, content 4 18% acids....

Abstract Since its cloning and commercial availability, applications of green fluorescent protein (GFP) as a reporter gene have become prevalent in many aspects science. The attributes GFP could also be applied to the area heterologous production. work described here represents first experiments use generic tool monitor production bioprocess development. We constructed plasmid containing an operon fusion two genes chloramphenicol acetyl transferase (CAT) . CAT served “model” recombinant...

We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using following motif: (His)(6)-GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively, (His)(6) a histidine affinity ligand purification, EK an enterokinase cleavage site recovering protein-of-interest from fusion. The CAT OPH products ( approximately 63 kDa GFP/CAT 70 GFP/OPH) were...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical nature of DNA-protein cross-links produced in mammalian chromatin by hydrogen peroxide the presence iron or copper ionsZeena Nackerdien, Govind Rao, Marco A. Cacciuttolo, Ewa Gajewski, and Miral DizdarogluCite this: Biochemistry 1991, 30, 20, 4873–4879Publication Date (Print):May 1, 1991Publication History Published online1 May 2002Published inissue 1...

INTRODUCTION: The impact of patient demographics and socioeconomic factors on healthcare outcomes in neurosurgical oncology is gaining greater recognition. METHODS: A retrospective analysis was conducted patients undergoing meningioma resection. Insurance status at time resection, preoperative health status, Modified Rankin Scores (mRS), Karnofsky Performance (KPS), tumor characteristics, radiographic findings from publicly (Medicare, Medicaid, VA) privately insured (all private insurer...

Ordinary Portland Cement (OPC) is a binding agent in concrete production. Despite the advantages OPC offers, its environmental impact cannot be overlooked. production contributes to over 11% of global carbon emissions and plays significant role climate change. Geopolymer, which involves alkali activation alumino-silicate materials, an alternative OPC. Rice Husk Ash (RHA), obtained by burning rice husks, has gained attention as sustainable material that can partially replace geopolymers. This...