A5030259389- Mast cells and histamine
- Food Allergy and Anaphylaxis Research
- Escherichia coli research studies
- Urinary Tract Infections Management
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Urinary Bladder and Prostate Research
- Fatty Acid Research and Health
- Monoclonal and Polyclonal Antibodies Research
- Caveolin-1 and cellular processes
- Glycosylation and Glycoproteins Research
- Polyamine Metabolism and Applications
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Bacterial Genetics and Biotechnology
- Antimicrobial Peptides and Activities
- Allergic Rhinitis and Sensitization
- Influenza Virus Research Studies
- Metabolomics and Mass Spectrometry Studies
- Muscle metabolism and nutrition
- Lipid metabolism and biosynthesis
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Meat and Animal Product Quality
- Probiotics and Fermented Foods
Duke Medical Center
2015-2024
National University of Singapore
2015-2024
Duke University Hospital
2015-2024
Duke University
2015-2024
Duke-NUS Medical School
2013-2016
Laboratory of Molecular Genetics
2002-2014
National Institute of Pathology
2006
Weizmann Institute of Science
2005
Washington University in St. Louis
1992-1999
University of Oxford
1999
Type 1 pili are heteropolymeric mannosebinding fibers produced by all members of the Enterobacteriaceae family. The bulk fiber is composed FimA. Two macromolecular complexes responsible for mediating an interaction with mannose-containing receptors were purified from fimA- Escherichia coli mannose affinity chromatography and ion-exchange chromatography. One complex contained only mannose-binding adhesin, FimH, associated FimG, a minor component type pilus. In other FimG-FimH moiety was...
Caveolae are subcellular structures implicated in the import and transcytosis of macromolecules transmembrane signaling. To date, evidence for existence caveolae hematopoietic cells has been ambiguous. were detected microvilli intracellular vesicles cultured mouse bone marrow–derived mast (BMMCs). CD48, a receptor FimH-expressing (type 1 fimbriated) Escherichia coli , was specifically localized to plasmalemmal BMMCs. The involvement bacterial entry into BMMCs indicated because...
Mast cells are well known for their harmful role in IgE-mediated hypersensitivity reactions, but physiological remains a mystery. Several recent studies have reported that mast play critical innate immunity mice by releasing tumor necrosis factor α (TNF-α) to recruit neutrophils sites of enterobacterial infection. In some cases, the cell TNF-α response was triggered when these directly bound FimH on surface Escherichia coli . We identified CD48, glycosylphosphatidylinositol-anchored...
Rabbit antibodies against components of the human milk fat globule bind specifically to normal breast epithelial cells and cell lines derived from carcinomas, as well outer surface globule. Variation in indirect immunofluorescence staining both intensity per percentage stained is observed for different brest lines. Cells other ectodermal tissues, fetal fibroblasts, blood buffy coat, even fibroblasts itself do not antibodies. This suggests that these are detecting cell-type-specific antigens....
Most studies of mast cells have been directed at their role in the pathophysiology IgE-mediated allergic reactions with little recognition participation bacterial infections. We report that can specifically bind FimH, a mannose-binding subunit on type 1 fimbriae expressed by Escherichia coli and other enterobacteria. This interaction triggers cell phagocytosis killing bacteria within vacuoles through release superoxide anions. Also, view fact capacity to inflammatory mediators are...
ABSTRACT The fungal secondary metabolite gliotoxin produced by Aspergillus fumigatus has been hypothesized to be important in the development of invasive aspergillosis. In this study, we addressed hypothesis disrupting a nonribosomal peptide synthetase (NRPS) (encoded gliP ) predicted involved production. Mutants with disrupted locus failed produce gliotoxin, which confirmed role NRPS encoded biosynthesis. We found no morphological, developmental, or physiological defects Δ mutant strains....
A wealth of evidence supports the essential contributions mast cells (MCs) to immune defense against bacteria and parasites; however, role MCs in viral infections has not been defined. We now report that rodent, monkey, human are able detect dengue virus (DENV), a lymphotropic, enveloped, single-stranded, positive-sense RNA results MC activation degranulation. observe response DENV also involves antiviral intracellular host pathways, melanoma differentiation-associated gene 5 (MDA5) retinoic...
Abstract Stimulated mast cells release a variety of chemotactic factors such as tumor necrosis factor α (TNF-α) and leukotriene B4. Recent studies have shown that cell-derived TNF-α plays critical role in host defense against Gram negative bacterial infections by the recruitment neutrophils to sites infection. In present study, we sought investigate if (LT) B4 response bacteria and, so, establish its vivo relevance. We show significant amounts LTB4 LTC4 exposure FimH-expressing type 1...
The relationship between the variability in fibronectin (Fn) content on human buccal epithelial cells and capacity of to bind gram-positive (Streptococcus pyogenes) or gram-negative (Escherichia coli Pseudomonas aeruginosa) bacteria was investigated. Adhesion experiments performed with mixtures mixed suspensions either S. pyogenes E. P. aeruginosa exhibited three major populations cells: one these able (gram positive) but neither bacteria; a second population not third various numbers both...