A5061920028- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Monoclonal and Polyclonal Antibodies Research
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Chromosomal and Genetic Variations
- Escherichia coli research studies
- Viral Infections and Immunology Research
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- Cancer Research and Treatments
- RNA regulation and disease
- Microbial Community Ecology and Physiology
- Fungal Infections and Studies
- DNA Repair Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Tuberculosis Research and Epidemiology
University at Albany, State University of New York
2015-2024
Texas Children's Hospital
2023
Albany State University
1989-2019
John Wiley & Sons (United States)
2019
Hudson Institute
2019
New York State Department of Health
2004-2018
Wadsworth Center
2005-2018
Institute of Biological, Environmental and Rural Sciences
2016
Children's Hospital of Philadelphia
2014
Rensselaer Polytechnic Institute
1997-2013
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes gene products. It provides explicit categories the many different Type II enzymes now identified a system naming putative found by sequence analysis of microbial genomes.
Journal Article Protein splicing elements: inteins and exteins — a definition of terms recommended nomenclature Get access Francine B. Perler, Perler * *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Elaine O. Davis, Davis 1National Institute Medical ResearchMill Hill, London, UK Gary E. Dean, Dean 2University Cincinnati College MedicineCincinnati, OH 45267-0524 Frederick S. Gimble, Gimble 3Institute Biosciences...
Journal Article Structural conventions for group I introns Get access John M. Burke, Burke * To whom correspondence and reprint requests should be addressed Search other works by this author on: Oxford Academic PubMed Google Scholar Mariene Belfort, Belfort 1Wadsworth Center Laboratories Research, New York State Department of HealthAlbany, NY 12201 Thomas R. Cech, Cech 2Department Chemistry Biochemistry, University ColoradoBoulder, CO 80309, USA Wayne Davies, Davies 3Allelix, Inc.6850...
DsrA is an 87-nt untranslated RNA that regulates both the global transcriptional silencer and nucleoid protein H-NS stationary phase stress response sigma factor RpoS (σ s ). We demonstrate acts via specific RNA:RNA base pairing interactions at hns locus to antagonize translation. also give evidence supports a role for rpoS enhance Negative regulation of by achieved interaction blocking translation RNA. In contrast, results suggest positive occurs formation structure activates cis -acting...
The Lactococcus lactis group II intron Ll.ltrB is similar to mobile yeast mtDNA introns, which encode reverse transcriptase, RNA maturase, and DNA endonuclease activities for site-specific insertion. Here, we show that the Lactococcal can be expressed spliced efficiently in Escherichia coli. intron-encoded protein LtrA has transcriptase maturase activities, with latter activity shown both vivo vitro, a first any protein. As of associated RNP particles containing excised RNA. Also, cleaves...
The continuous sequence of 2.3 kilobases in a 3-kilobase DNA fragment encoding the structural gene for coliphage T4 thymidylate synthase (5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC 2.1.1.45) was determined by using M13 dideoxy chain-termination method. From coding information within this and that provided analysis selected CNBr peptides from protein product, primary structure determined. most significant finding these studies is presence 1017-base-pair interruption...
DsrA is an 87-nucleotide regulatory RNA of Escherichia coli that acts in trans by RNA–RNA interactions with two different mRNAs, hns and rpo S. has opposite effects on these transcriptional regulators. H-NS levels decrease, whereas RpoS (σ s ) increase. Here we show enhances mRNA turnover yet stabilizes S mRNA, either directly or via translation. Computational footprinting approaches led to a refined structure for DsrA, model which interacts the start stop codon regions form coaxial stack....
To address the effect of host proteins on self-splicing properties group I introns bacteriophage T4, we have purified an activity from Escherichia coli extracts that facilitates both trans- and cis-splicing T4 in vitro. The is attributable to a number proteins, several which are ribosomal proteins. Although these variable abilities stimulate splicing, protein S12 most effective. mitigates negative effects splicing large internal open reading frames (ORFs) common introns. In contrast shown...
Group II intron homing occurs primarily by a mechanism in which the RNA reverse splices into DNA target site and is then transcribed intron-encoded protein. The recognized an RNP complex containing protein excised RNA. Here, we analyzed target-site requirements for Lactococcus lactis Ll.LtrB group vitro vivo. Our results suggest model similar to yeast mtDNA introns, first recognizes small number of nucleotide residues double-stranded causes unwinding, enabling base-pair with splicing....
Inteins are protein-splicing elements, most of which contain conserved sequence blocks that define a family homing endonucleases. Like group I introns encode such endonucleases, inteins mobile genetic elements. Recent crystallography and computer modeling studies suggest consist two structural domains correspond to the endonuclease To determine whether bipartite structure is mirrored by functional independence domain, entire component was deleted from Mycobacterium tuberculosis recA intein....