A5100360409- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Viral Infections and Immunology Research
- RNA Research and Splicing
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- X-ray Diffraction in Crystallography
- Acute Myocardial Infarction Research
- Crystallization and Solubility Studies
- Genetics and Neurodevelopmental Disorders
- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
- Coronary Interventions and Diagnostics
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Cardiac Imaging and Diagnostics
- Calcium signaling and nucleotide metabolism
- Adenosine and Purinergic Signaling
- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Animal Disease Management and Epidemiology
- Ion Channels and Receptors
Australian Regenerative Medicine Institute
2019-2025
Monash University
2019-2025
Zhujiang Hospital
2024-2025
Southern Medical University
2024-2025
The University of Adelaide
2024-2025
Guangzhou Medical University
2024-2025
Chinese Academy of Sciences
2001-2025
Nanjing Tech University
2013-2025
Fuzhou University
2025
Fujian Institute of Research on the Structure of Matter
2025
Macrophage-associated immune response plays an important role in myocardial ischemia/reperfusion (IR) injury. Dectin-1, expressed mainly on activated myeloid cells, is crucial for the regulation of homeostasis as a pattern recognition receptor. However, its effects and roles during IR injury remain unknown. Genetic ablation, antibody blockade, or Dectin-1 activation, along with adoptive bone marrow transfer chimeric model, was used to determine functional significance Immune cell filtration...
The histone demethylase PHF8 has been implicated in multiple pathological disorders, including X-linked mental retardation and tumorigenesis. However, it is not clear how the abundance function of are regulated. Here, we report that physically associates with deubiquitinase USP7. Specifically, demonstrated USP7 promotes deubiquitination stabilization PHF8, leading to upregulation a group genes, cyclin A2, critical for cell growth proliferation. USP7-encoding gene was also transcriptionally...
RNA has been implicated in the recruitment of chromatin modifiers, and previous studies have provided evidence favor against this idea. RNase treatment is commonly used to study RNA-mediated regulation but limitations approach remain unclear. A during immunoprecipitation (ChIP) reduces occupancy H3K27me3 methyltransferase Polycomb repressive complex 2 (PRC2). This led suggestions an "RNA bridge" between PRC2 chromatin. Here, we show that ChIP causes apparent loss all facultative...
The compaction of chromatin is a prevalent paradigm in gene repression. Chromatin commonly thought to repress transcription by restricting accessibility. However, the spatial organization and dynamics compacted gene-repressing factors are unknown. Here, using cryo-electron tomography, we solved three-dimensional structure condensed polycomb repressive complex 1 (PRC1) with CBX8. PRC1-condensed porous stabilized through multivalent dynamic interactions PRC1 chromatin. Mechanistically,...
Expression of apoptotic protease activating factor-1 (Apaf-1) gradually decreases during brain development, and this decrease is likely responsible for the decreased sensitivity tissue to apoptosis. However, mechanism by which Apaf-1 expression remains elusive. In present study, we found that four microRNAs (miR-23a/b miR-27a/b) miR-23a-27a-24 miR-23b-27b-24 clusters play key roles in modulating Apaf-1. First, miR-23a/b miR-27a/b suppressed vitro. Interestingly, miR-23-27-24 mouse cortex...
Abstract PD-1 (CD279)–PD-L1 (CD274) inhibitory signaling is critical for cancer immune evasion, and thus has become one of the major targets in anticancer immunotherapy. There are several studies that demonstrate potent effects posttranslational modifications CD274 on inactivation suppression, such as ubiquitination, phosphorylation, glycosylation, palmitoylation. However, regulatory mechanisms deubiquitination still largely unclear. Here, we identified ubiquitin-specific protease 22 (USP22)...
Abstract Defective centrosome duplication is implicated in microcephaly and primordial dwarfism as well various ciliopathies cancers. Yet, how the biogenesis regulated remains poorly understood. Here we report that X-linked deubiquitinase USP9X physically associated with centriolar satellite protein CEP131, thereby stabilizing CEP131 through its activity. We demonstrate an integral component of required for biogenesis. Loss-of-function impairs gain-of-function promotes amplification...
Abstract Ferric uptake regulator (Fur) plays a key role in the iron homeostasis of prokaryotes, such as bacterial pathogens, but molecular mechanisms and structural basis Fur–DNA binding remain incompletely understood. Here, we report high-resolution structures Magnetospirillum gryphiswaldense MSR-1 Fur four different states: apo-Fur, holo-Fur, Fur– feoAB1 operator complex Pseudomonas aeruginosa box complex. Apo-Fur is transition metal ion-independent dimer whose induces profound...
Central to the recognition, signaling, and repair of DNA double-strand breaks (DSBs) are MRE11-RAD50-NBS1 (MRN) complex mediator damage checkpoint protein 1 (MDC1), interplay which is essential for initiation amplification response (DDR). The intrinsic rule governing regulation function this molecular machinery remains be investigated. We report here that ubiquitin-specific protease USP7 was physically associated with MRN-MDC1 acted as a platform efficiently deubiquitinate stabilize MDC1,...
As a neurotropic virus, human Enterovirus 71 (EV71) infection causes hand-foot-and-mouth disease (HFMD) and may develop severe neurological disorders in infants. Toll-like receptor 7 (TLR7) acts as an innate immune is also death the central nervous system (CNS). However, mechanisms underlying regulation of TLR7-mediated brain pathogenesis upon EV71 remain largely elusive. Here we reveal novel mechanism by which infects astrocytes induces neural via TLR7 interleukin-6 (IL-6) C57BL/6 mice...
The central region of MDM2 is critical for p53 activation and tumor suppression. Upon ribosomal stress, this bound by proteins, particularly protein L11 (RPL11), leading to inactivation subsequent activation. Here, we solved the complex structure human MDM2–RPL11 at 2.4 Å. extensively interacts with RPL11 through an acidic domain two zinc fingers. Formation induces substantial conformational changes in both proteins. RPL11, unable bind mutants, fails induce cells. mimics 28S rRNA binding...
Abstract Polycomb repressive complex 2 (PRC2) interacts with RNA in cells, but there is no consensus on how regulates PRC2 canonical functions, including chromatin modification and the maintenance of transcription programs lineage-committed cells. We assayed two separation-of-function mutants catalytic subunit EZH2, defective binding functional methyltransferase activity. find that part RNA-binding surface EZH2 required for modification, yet this activity independent RNA. Mechanistically,...