A5011142547- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Hepatitis C virus research
- Viral Infections and Immunology Research
- Hepatitis B Virus Studies
- COVID-19 Clinical Research Studies
- Inflammasome and immune disorders
- Immune Response and Inflammation
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- RNA Research and Splicing
- RNA regulation and disease
- Cytokine Signaling Pathways and Interactions
- Liver Disease Diagnosis and Treatment
- SARS-CoV-2 detection and testing
- Long-Term Effects of COVID-19
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Immune responses and vaccinations
- Inflammatory mediators and NSAID effects
- Cancer-related Molecular Pathways
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- Cholesterol and Lipid Metabolism
Wuhan University
2015-2025
Zhongnan Hospital of Wuhan University
2012-2013
Wuhan Institute of Bioengineering
2012-2013
University of Minnesota Medical Center
2009-2012
NL63 coronavirus (NL63-CoV), a prevalent human respiratory virus, is the only group I known to use angiotensin-converting enzyme 2 (ACE2) as its receptor. Incidentally, ACE2 also used by II SARS (SARS-CoV). We investigated how different groups of coronaviruses recognize same receptor, whereas homologous receptors. determined crystal structure NL63-CoV spike protein receptor-binding domain (RBD) complexed with ACE2. RBD has novel β-sandwich core consisting layers β-sheets, presenting 3...
Upon recognition of viral components by pattern receptors, such as the toll-like receptors (TLRs) and retinoic acid-inducible gene I (RIG-I)-like helicases, cells are activated to produce type interferon (IFN) proinflammatory cytokines. These pathways tightly regulated host prevent an inappropriate cellular response, but viruses can modulate these proliferate spread. In this study, we revealed a novel mechanism in which hepatitis C virus (HCV) evades immune surveillance system activating...
Zika virus (ZIKV) infection is a public health emergency and host innate immunity essential for the control of infection. The NLRP3 inflammasome plays key role in immune responses by activating caspase-1 to facilitate interleukin-1β (IL-1β) secretion. Here we report that ZIKV stimulates IL-1β secretion infected patients, human PBMCs macrophages, mice, mice BMDCs. knockdown cells knockout inhibit ZIKV-mediated secretion, indicating an ZIKV-induced activation. Moreover, NS5 protein required...
Abstract Cytokine storm induced by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a major pathological feature of Disease 2019 (COVID-19) and crucial determinant in COVID-19 prognosis. Understanding the mechanism underlying SARS-CoV-2-induced cytokine critical for control. Here, we identify that SARS-CoV-2 ORF3a host hypoxia-inducible factor-1α (HIF-1α) play key roles virus infection pro-inflammatory responses. RNA sequencing shows HIF-1α signaling, immune response,...
One of the fundamental reactions innate immune responses to pathogen infection is release pro-inflammatory cytokines, including IL-1β, processed by NLRP3 inflammasome. The stimulator interferon genes (STING) has essential roles in response against infections. Here we reveal a distinct mechanism which STING regulates inflammasome activation, IL-1β secretion, and inflammatory human cell lines, mice primary cells, mice. Interestingly, upon HSV-1 cytosolic DNA stimulation, binds promotes...
SARS-CoV-2 is highly pathogenic in humans and poses a great threat to public health worldwide. Clinical data shows disturbed type I interferon (IFN) response during the virus infection. In this study, we discovered that nucleocapsid (N) protein of plays an important role inhibition beta (IFN-β) production. N repressed IFN-β production induced by poly(I:C) or upon Sendai (SeV) We noted also suppressed production, several signaling molecules downstream retinoic acid-inducible gene (RIG-I)...
The Systemic Immune-Inflammation Index (SII) is a quantitative measurement of the systemic immune-inflammatory response in human body. SII has been shown to have prognostic value various clinical settings, including critical illness, sepsis, and cancer. Its role chronic obstructive pulmonary disease (COPD) remains unclear requires further investigation.We analyzed demographic data from 16,636 participants National Health Nutrition Examination Survey. Logistic regression analysis was...
Abstract Background Among patients with coronavirus disease 2019 (COVID-19), the cases of a significant proportion are severe. A viral nucleic acid test is used for diagnosis COVID-19, and some hematological indicators have been in auxiliary identification severity COVID-19. Regarding body fluid samples, except being testing, relationship between COVID-19 routine parameters not known. Our aim was to investigate value urine biochemical prediction Methods total 119 were enrolled at Renmin...
As a neurotropic virus, human Enterovirus 71 (EV71) infection causes hand-foot-and-mouth disease (HFMD) and may develop severe neurological disorders in infants. Toll-like receptor 7 (TLR7) acts as an innate immune is also death the central nervous system (CNS). However, mechanisms underlying regulation of TLR7-mediated brain pathogenesis upon EV71 remain largely elusive. Here we reveal novel mechanism by which infects astrocytes induces neural via TLR7 interleukin-6 (IL-6) C57BL/6 mice...
Aberrant activity of NLRP3 has been shown associations with severe diseases. Palmitoylation is a kind protein post-translational modification, which to regulate cancer development and the innate immune system. Here, we showed that palmitoylated at Cys419 palmitoyltransferase ZDHHC17 predominant enzyme mediates palmitoylation promotes activation by interacting facilitating NIMA-related kinase 7 (NEK7)-NLRP3 interactions. Blockade inhibitor, 2-bromopalmitate, effectively inhibited in vitro....
Enterovirus 71 (EV71) infection causes hand, foot, and mouth disease (HFMD), even fatal neurological complications. However, the mechanisms underlying EV71 pathogeneses are largely unknown. This study reveals a distinct mechanism by which induces apoptosis autophagy in neural cells. non-structure protein 3D (also known as RNA-dependent RNA polymerase, RdRp) interacts with peroxisomal acyl-CoA oxidase 1 (ACOX1), contributes to ACOX1 downregulation. Further studies demonstrate that reduces...
Hepatitis B virus (HBV) infection may cause acute hepatitis B, chronic (CHB), liver cirrhosis, and hepatocellular carcinoma (HCC). However, the mechanisms by which HBV evades host immunity maintains are largely unknown. Here, we revealed that matrix metalloproteinase 9 (MMP-9) is activated in peripheral blood mononuclear cells (PBMCs) of HBV-infected patients, stimulates MMP-9 expression macrophages PBMCs isolated from healthy individuals. plays important roles breakdown extracellular...
Dengue virus (DENV) infection causes serious clinical symptoms, including dengue hemorrhagic fever (DHF) and shock syndrome (DSS). Vascular permeability change is the main feature of diseases, abnormal expression proinflammatory cytokines important cause vascular change. However, mechanism underlying induced by DENV has not been fully elucidated. Here, we reveal a distinct which promotes NLRP3 inflammasome activation interleukin-1 beta (IL-1β) release to induce endothelial leakage in mice. M...
Enterovirus 71 (EV71) is one causative agent of hand, foot, and mouth disease (HFMD), which may lead to severe neurological disorders mortality in children. EV71 genome a positive single-stranded RNA containing single open reading frame (ORF) flanked by 5′-untranslated region (5′UTR) 3′UTR. The 5′UTR fundamentally important for virus replication interacting with cellular proteins. Here, we revealed that poly(C)-binding protein 1 (PCBP1) specifically binds the EV71. Detailed studies indicated...
Hepatitis C virus (HCV) infection is a leading cause of chronic liver diseases and hepatocellular carcinoma (HCC) Golgi protein 73 (GP73) serum biomarker for HCC. However, the mechanism underlying GP73 regulates HCV largely unknown. Here, we revealed that acts as novel negative regulator host innate immunity to facilitate infection. expression activated correlated with interferon-beta (IFN-β) production during in patients' serum, primary human hepatocytes (PHHs) hepatoma cells through...
Abstract Background As the increasing number of Corona Virus Disease 2019 (COVID-19) patients caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), which an outbreak initiated from Wuhan, China in December, 2019, clinical features and treatment COVID-19 have been understood. However, it is urgent to need rapid accurate detection for SARS-CoV-2 infection diagnosis. We aimed evaluate antibodies-based nucleic acid-based tests (NAT) SARS-CoV-2-infected patients. Method retrospectively...
Coronavirus disease 2019 (COVID-19) causes pulmonary edema, which disrupts the lung alveoli-capillary barrier and leads to cell apoptosis, main cause of death. However, molecular mechanism behind SARS-CoV-2's apoptotic activity remains unknown. Here, we revealed that SARS-CoV-2-ORF-3a mediates pathology associated with SARS-CoV-2, is demonstrated by fact it tissue damage. The in vitro results showed triggers death via disruption mitochondrial homeostasis, modulated through regulation...