A5100322666- Ion channel regulation and function
- Cleft Lip and Palate Research
- Genetic and Kidney Cyst Diseases
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Craniofacial Disorders and Treatments
- Biomedical Research and Pathophysiology
- Biochemical Analysis and Sensing Techniques
- Ion Channels and Receptors
- Congenital Anomalies and Fetal Surgery
- Tracheal and airway disorders
- Viral Infections and Immunology Research
- Porphyrin Metabolism and Disorders
- Renal and related cancers
- Regulation of Appetite and Obesity
- Human Health and Disease
- Lepidoptera: Biology and Taxonomy
- Sodium Intake and Health
- Spider Taxonomy and Behavior Studies
- RNA and protein synthesis mechanisms
- Nutrition, Genetics, and Disease
- Iron Metabolism and Disorders
- Animal Nutrition and Physiology
- Ion Transport and Channel Regulation
- Calcium signaling and nucleotide metabolism
Guangdong Academy of Agricultural Sciences
2013-2024
Chinese PLA General Hospital
2005-2024
Guangdong Polytechnic of Science and Technology
2024
St. John's University
2019-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Sichuan University
2006-2023
The University of Texas Southwestern Medical Center
2022
Harbin Medical University
2022
Zhengzhou University
2021
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2020
Intracellular calcium (Ca2+) inhibits the opening of L-type (alpha 1C) Ca2+ channels, providing physiological control entry into a wide variety cells. A structural determinant this Ca(2+)-sensitive inactivation was revealed by chimeric channels derived from parental alpha 1C and 1E latter which is neuronal channel lacking inactivation. consensus Ca(2+)-binding motif (an EF hand), located on subunit, required for Donation EF-hand region to conferred Ca(2+)-inactivating phenotype. These...
Transient receptor potential mucolipin 1 (TRPML1) is a Ca2+-permeable, nonselective cation channel ubiquitously expressed in the endolysosomes of mammalian cells and its loss-of-function mutations are direct cause type IV mucolipidosis (MLIV), an autosomal recessive lysosomal storage disease. TRPML1 ligand-gated that can be activated by phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2] as well some synthetic small-molecule agonists. Recently, rapamycin has also been shown to directly bind...
Autosomal dominant polycystic kidney disease (ADPKD) is caused by mutations in PKD1 or PKD2 gene, encoding the protein polycystin-1 and transient receptor potential channel polycystin-2 (also known as TRPP2), respectively. Polycystin-1 form a receptor-ion complex located primary cilia. The function of this complex, especially role polycystin-1, largely unknown due to lack reliable functional assay. In study, we dissect directly recording currents mediated gain-of-function (GOF)...
The ability to add noncanonical amino acids the genetic code may allow one evolve proteins with new or enhanced properties using a larger set of building blocks. To this end, we have been able select mutant thermal from library E. coli homoserine O-succinyltransferase (metA) mutants containing randomly incorporated acids. Here, show that substitution Phe 21 (p-benzoylphenyl)alanine (pBzF), increases melting temperature metA by °C. This dramatic increase in stability, arising single mutation,...
Tolerance to drugs that affect neural activity is mediated, in part, by adaptive mechanisms attempt restore normal excitability. Changes the expression of ion channel genes are thought play an important role these adaptations. The slo gene encodes pore-forming subunit BK-type Ca2+-activated K+ channels, which regulate many aspects activity. Given induction plays acquisition tolerance sedating drugs, we investigated molecular mechanism induction. Using chromatin immunoprecipitation followed...
Abstract The heteromeric complex between PKD1L3, a member of the polycystic kidney disease (PKD) protein family, and PKD2L1, also known as TRPP2 or TRPP3, has been prototype for mechanistic characterization heterotetrametric TRP-like channels. Here we show that truncated PKD1L3/PKD2L1 with C-terminal TRP-fold fragment PKD1L3 retains both Ca 2+ acid-induced channel activities. Cryo-EM structures this core heterocomplex without supplemented were determined at resolutions 3.1 Å 3.4 Å,...
Abstract The R-type voltage-gated Ca 2+ (Ca v ) channels 2.3, widely expressed in neuronal and neuroendocrine cells, represent potential drug targets for pain, seizures, epilepsy, Parkinson’s disease. Despite their physiological importance, there have lacked selective small-molecule inhibitors targeting these channels. High-resolution structures may aid rational design. Here, we report the cryo-EM structure of human 2.3 complex with α2δ−1 β3 subunits at an overall resolution 3.1 Å. is nearly...
We have studied the effects of activated G proteins (Gs alpha and Gi1 alpha), adenosine 3',5'-cyclic monophosphate-dependent protein kinase (PKA), okadaic acid on L-type Ca channels incorporated from porcine ventricular sarcolemma into planar lipid bilayers. Channel activity evoked by membrane depolarizations diminished to extremely low levels within 2 min incorporation (channel "rundown"). When Gs [activated with guanosine 5'-O-(3-thiotriphosphate)] was present in intracellular chamber,...
Autosomal dominant polycystic liver disease (PCLD) is caused by mutations of either PRKCSH or Sec63, two proteins associated with the endoplasmic reticulum (ER). Both are involved in carbohydrate processing, folding and translocation newly synthesized glycoproteins. It postulated that defective quality control initiates reticulum-associated degradation (ERAD), which disrupts hepatic homeostasis patients Sec63 mutations. However, precise molecular mechanisms not known. Here, we show...
Mobilization of iron stored in the interior cavity BfrB requires electron transfer from [2Fe–2S] cluster Bfd to core BfrB. A crystal structure Pseudomonas aeruginosa BfrB:Bfd complex revealed that can bind up 12 molecules at structurally identical binding sites, placing each immediately above a heme group [Yao, H., et al. (2012) J. Am. Chem. Soc., 134, 13470–13481]. We report here study aimed characterizing strength P. association using surface plasmon resonance and isothermal titration...
TRPP2, also known as polycystin-2, is a calcium permeable nonselective cation channel that mutated in autosomal dominant polycystic kidney disease but has been implicated the regulation of cardiac development, renal tubular differentiation, and left-to-right (L-R) axis determination. For obtaining further insight into how TRPP2 exerts tissue-specific functions, this study took advantage PACS-dependent trafficking zebrafish larvae. PACS proteins recognize an acidic cluster within...
Background Cleft lip (CL) and cleft palate (CP) are two of the most frequent congenital malformations. Many epidemiologic studies on this deformity have been conducted worldwide, often producing inconsistent results. This study assessed epidemiology some genetic aspects in a Chinese sample from Smile Train Program to compare with other methodologically sound surveys. Methods The general information, family history, classification associated malformations 8000 CL CP surgery patients were...
Walnuts are seeds with a hard shell from the genus Juglans (J. mandshurica, J. regia, sinensis, cathayensis, nigra and sigillata). can nourish brain cells to improve human memory. Other parts of plant also employed as traditional Chinese medicines. Modern research on species has been mostly focused above-mentioned species, which all called walnuts. have diverse chemical constituents, including diarylheptanoids, quinones, polyphenols, flavones terpenes. The diarylheptanoids quinones notable...
Coxsackievirus B (CVB) is one of the major viral pathogens human myocarditis and cardiomyopathy without any effective preventive measures; therefore, it necessary to develop a safe efficacious vaccine against CVB. Immunoinformatics methods are both economical convenient as in-silico simulations can shorten development time. Herein, we design novel multi-epitope for prevention CVB by using immunoinformatics methods. With help advanced approaches, predicted different B-cell, cytotoxic T...
Background: Velopharyngeal inadequacy (VPI), which has a significant negative impact on speech intelligibility and resonance quality, may be caused by physiological inadequacy. The current study aimed to investigate the maximal velar pharyngeal motions levator muscle shortening in children with repaired cleft palate different outcomes as well without using magnetic imaging techniques general anesthesia. Methods: Three groups of sex- age-matched were recruited: adequate velopharyngeal...
Abstract Background Hyperpigmentation of the visceral peritoneum (HVP) has recently garnered much attention in poultry industry because possible risk to health affected animals and damage it causes appearance commercial chicken carcasses. However, heritable characters HVP remain unclear. The objective this study was investigate genetic parameters by genome-wide association (GWAS) chickens. Results found be influenced factors, with a heritability score 0.33. had positive correlations growth...
Smoking is a complex behavior with heritability as high 50%. Given such large genetic contribution, it provides an opportunity to prevent those individuals who are susceptible smoking dependence from ever starting smoke by predicting their inherited predisposition genomic profiles. Although previous studies have identified many susceptibility variants for smoking, they limited power predict behavior. We applied the support vector machine (SVM) and random forest (RF) methods build prediction...
Otopetrin (Otop) proteins were recently found to function as proton channels, with Otop1 revealed be the sour taste receptor in mammals. Otop contain twelve transmembrane segments (S1-S12) which are divided into structurally similar N and C domains. The mechanisms by channels sense extracellular protons initiate gating conduct once activated remains largely elusive. Here we show that two loops playing key roles human channel function. We find residue H229 S5-S6 loop is critical for sensing...