A5103275896- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Genetics and Neurodevelopmental Disorders
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- Cancer-related Molecular Pathways
- Genomics and Phylogenetic Studies
- Autophagy in Disease and Therapy
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- Cancer-related gene regulation
- Bioinformatics and Genomic Networks
- PARP inhibition in cancer therapy
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- Microtubule and mitosis dynamics
- Frailty in Older Adults
- Sirtuins and Resveratrol in Medicine
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Toxoplasma gondii Research Studies
Tianjin Medical University General Hospital
2021-2023
Tianjin Medical University Cancer Institute and Hospital
2018-2022
Henan Agricultural University
2022
Tianjin Medical University
2014-2020
Carnegie Mellon University
2005
The histone demethylase PHF8 has been implicated in multiple pathological disorders, including X-linked mental retardation and tumorigenesis. However, it is not clear how the abundance function of are regulated. Here, we report that physically associates with deubiquitinase USP7. Specifically, demonstrated USP7 promotes deubiquitination stabilization PHF8, leading to upregulation a group genes, cyclin A2, critical for cell growth proliferation. USP7-encoding gene was also transcriptionally...
Significance DNA double-strand breaks are generally repaired in the context of highly organized chromatin. However, how epigenetic mechanisms involved maintenance genetic fidelity remains poorly understood. Here we report that lysine-specific histone demethylase 5B (KDM5B), a well-defined transcriptional repressor, promotes break signaling and is required for efficient repairs. We demonstrated KDM5B, doing so, functions to orchestrate checkpoint activation cell survival after damage. Our...
Abstract Defective centrosome duplication is implicated in microcephaly and primordial dwarfism as well various ciliopathies cancers. Yet, how the biogenesis regulated remains poorly understood. Here we report that X-linked deubiquitinase USP9X physically associated with centriolar satellite protein CEP131, thereby stabilizing CEP131 through its activity. We demonstrate an integral component of required for biogenesis. Loss-of-function impairs gain-of-function promotes amplification...
Central to the recognition, signaling, and repair of DNA double-strand breaks (DSBs) are MRE11-RAD50-NBS1 (MRN) complex mediator damage checkpoint protein 1 (MDC1), interplay which is essential for initiation amplification response (DDR). The intrinsic rule governing regulation function this molecular machinery remains be investigated. We report here that ubiquitin-specific protease USP7 was physically associated with MRN-MDC1 acted as a platform efficiently deubiquitinate stabilize MDC1,...
Histone chaperone ASF1A has been reported to be dysregulated in multiple tumors; however, the underlying molecular mechanism that how abundance and function of are regulated remains unclear. Here we report is physically associated with USP52, which previously identified as a pseudo-deubiquitinase. Interestingly, demonstrate USP52 bona fide ubiquitin-specific protease, promotes deubiquitination stabilization. USP52-promoted stabilization facilitates chromatin assembly favors cell cycle...
PHF8-promoted non-histone methylation switch plays a critical role in replication stress response and genome integrity.
Cell division cycle 123 (CDC123) has been implicated in a variety of human diseases. However, it remains unclear whether CDC123 plays role tumorigenesis and how its abundance is regulated. In this study, we found that was highly expressed breast cancer cells, high expression positively correlated with poor prognosis. Knowndown impaired the proliferation cells. Mechanistically, identified deubiquitinase, ubiquitin-specific peptidase 9, X-linked (USP9X), could physically interact...
Single-stranded DNA (ssDNA) coated with replication protein A (RPA) acts as a key platform for the recruitment and exchange of genome maintenance factors in damage response. Yet, how formation ssDNA-RPA intermediate is regulated remains elusive.
The NAD+ -dependent protein deacetylase silent information regulator 1 (SIRT1) targets multiple proteins for deacetylation, and it has been implicated in a variety of cellular pathways human diseases. However, remains unclear how the abundance SIRT1 is regulated. Here, by mass spectrometry analysis SIRT1-containing complexes, we revealed that physically associated with ubiquitin-specific protease USP7. Importantly, found USP7 cleaves K48-linked polyubiquitin chains promotes stabilization....
Rationale: A number of guanine nucleotide exchange factors (GEFs) including epithelial cell transforming factor ECT2 are believed to drive carcinogenesis through activating distinct oncogenic GTPases. Yet, whether GEF-independent activity also plays a role in tumorigenesis remains unclear. Methods: Immunohistochemical (IHC) staining, colony formation and xenograft assays were used examine the breast carcinogenesis. Co-immunoprecipitation, immunofluorescent stainings, vivo deubiquitination...
GATA3 is a key transcription factor in cell fate determination and its dysregulation has been implicated various types of malignancies. However, how the abundance function are regulated remains unclear. Here, we report that physically associated with FBXW7α, FBXW7α destabilizes through assembly SKP1‐CUL1‐F‐box E3 ligase complex. Importantly, showed promotes ubiquitination degradation GSK3 dependent manner. Furthermore, demonstrated inhibits breast cancer cells survival destabilizing GATA3,...
The checkpoint kinase ATR is a master regulator of DNA damage response. Yet, how activity regulated remains to be investigated. We report here that histone demethylase PHF8 critically required for activation and cellular response ATR-activating lesions. With combined approach structural biochemical analysis, we unveiled an extraordinary feature the association/dissociation with TOPBP1, essential allosteric activator ATR. removes TOPBP1 K118 mono-methylation facilitate association RAD9, thus...