A5076238689- RNA modifications and cancer
- RNA Research and Splicing
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- interferon and immune responses
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Genomics and Chromatin Dynamics
- Protein Degradation and Inhibitors
- Animal Virus Infections Studies
- CAR-T cell therapy research
- Bacteriophages and microbial interactions
- Neuroendocrine regulation and behavior
- Insect and Arachnid Ecology and Behavior
- Neurobiology and Insect Physiology Research
California University of Pennsylvania
2020-2024
Children's Hospital of Philadelphia
2022-2024
University of Pennsylvania
2019-2023
Philadelphia University
2020
Stable isotope labeling by amino acids in cell culture (SILAC) coupled to data-dependent acquisition (DDA) is a common approach quantitative proteomics with the desirable benefit of reducing batch effects during sample processing and data acquisition. More recently, using data-independent (DIA/SWATH) systematically measure peptides has gained popularity for its comprehensiveness, reproducibility, accuracy quantification. The complementary advantages these two techniques logically suggests...
Abstract Biomolecular condensates (BMCs) play important roles in diverse biological processes. Many viruses form BMCs which have been implicated various functions critical for the productive infection of host cells. The adenovirus L1-52/55 kilodalton protein (52K) was recently shown to viral that coordinate genome packaging and capsid assembly. Although packaging, we do not know how are regulated during infection. Here show phosphorylation serine residues 28 75 within N-terminal...
The discovery and analysis of modifications on proteins nucleic acids has provided functional information that rapidly accelerated the field epigenetics. While protein post-translational (PTMs), especially histones, have been highlighted as critical components epigenetics, post-transcriptional modification RNA a subject more recently emergent interest. Multiple known to be present in tRNA rRNA since 1960s, but exploration mRNA, small RNA, inducible remains nascent. Sequencing-based methods...
Adenovirus is a common human pathogen that relies on host cell processes for transcription and processing of viral RNA protein production. Although adenoviral promoters, splice junctions, polyadenylation sites have been characterized using low-throughput biochemical techniques or short read cDNA-based sequencing, these technologies do not fully capture the complexity transcriptome. By combining Illumina short-read nanopore long-read direct sequencing approaches, we mapped start cleavage...
Abstract Temporal control of protein levels in cells and living animals can be used to improve our understanding function. In addition, engineered proteins could therapeutic applications. PRoteolysis-TArgeting Chimeras (PROTACs) have emerged as a small-molecule-driven strategy achieve rapid, post-translational regulation abundance via recruitment an E3 ligase the target interest. Here, we develop several PROTAC molecules by covalently linking antibiotic trimethoprim (TMP) pomalidomide,...
Summary Transcription factors (TFs) orchestrate the gene expression programs that define each cell’s identity. The canonical TF accomplishes this with two domains, one binds specific DNA sequences and other protein coactivators or corepressors. We find at least half of TFs also bind RNA, doing so through a previously unrecognized domain sequence functional features analogous to arginine-rich motif HIV transcriptional activator Tat. RNA-binding contributes function by promoting dynamic...
Viral infections induce myriad changes to the host cell proteome. As viruses harness cellular processes and counteract defenses, they impact abundance, posttranslational modifications, interactions, or localization of proteins.
Post-transcriptional modifications of RNA strongly influence the structure and function. Recent advances in sequencing mass spectrometry (MS) methods have identified over 140 these on a wide variety species. Most next-generation approaches can only map one modification at time, while MS assign multiple simultaneously an unbiased manner, cannot accurately catalog complex biological samples due to limitations fragment length coverage depth. Thus, facile method identify novel locating them...
ABSTRACT Social insects offer powerful models to investigate the mechanistic foundation of elaborate individual behaviors comprising a cooperative community. Workers leafcutter ant genus Atta provide an extreme example behavioral segregation among many phenotypically distinct worker types. We utilize complex system cephalotes test molecular underpinnings programming and, in particular, extent plasticity reprogramming. identify specific neuropeptides as mediators division labor A. , finding...
ABSTRACT Stable isotope labeling by amino acids in cell culture (SILAC) coupled to data-dependent acquisition (DDA) is a common approach quantitative proteomics with the desirable benefit of reducing batch effects during sample processing and data acquisition. More recently, using data-independent (DIA/SWATH) systematically measure peptides has gained popularity for its comprehensiveness, reproducibility, accuracy quantification. The complementary advantages these two techniques logically...
ABSTRACT Viruses promote infection by hijacking host ubiquitin machinery to counteract or redirect cellular processes. Adenovirus encodes two early proteins, E1B55K and E4orf6, that together co-opt a ligase complex overcome defenses virus production. mutants lacking E4orf6 display defects in viral RNA processing protein production, but previously identified substrates of the redirected do not explain these phenotypes. Here we used quantitative proteomics approach identify...
Abstract Post-transcriptional modifications of RNA strongly influence structure and function. Recent advances in sequencing mass spectrometry (MS) methods have identified over 140 these on a wide variety species. Most next-generation approaches can only map one modification at time, while MS assign multiple simultaneously an unbiased manner, cannot accurately catalog complex biological samples due to limitations fragment length coverage depth. Thus, facile method identify novel locating them...