A5070243994- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Organic Chemistry Synthesis Methods
- Cancer-related molecular mechanisms research
- Classical Antiquity Studies
- RNA Interference and Gene Delivery
- ATP Synthase and ATPases Research
- Plant tissue culture and regeneration
- Peacebuilding and International Security
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Middle East and Rwanda Conflicts
- Cambodian History and Society
- Historical, Religious, and Philosophical Studies
- Chromosomal and Genetic Variations
- Cancer-related gene regulation
- Maritime Security and History
Australian Regenerative Medicine Institute
2019-2023
Monash University
2019-2023
University of North Carolina at Chapel Hill
2020-2021
UNC Lineberger Comprehensive Cancer Center
2020
University of Otago
2013
Norwich Research Park
2002
John Innes Centre
2002
Polycomb repressive complex 1 (PRC1) and PRC2 are responsible for epigenetic gene regulation. PRC1 ubiquitinates histone H2A (H2Aub), which subsequently promotes to introduce the H3 lysine 27 tri-methyl (H3K27me3) chromatin mark. Although this mechanism provides a link between two key transcriptional repressors, PRC2, it is unknown how histone-tail dynamics contribute process. Here, we have examined effect of ubiquitination linker-DNA on H3-tail H3K27 methylation by PRC2. In naïve...
Abstract Lung squamous carcinoma (LUSC) is a highly metastatic disease with poor prognosis. Using an integrated screening approach, we found that miR-671-5p reduces LUSC metastasis by inhibiting circular RNA (circRNA), CDR1as. Although the putative function of circRNA through miRNA sponging, more potently silenced axis CDR1as and its antisense transcript, cerebellar degeneration related protein 1 (CDR1). Silencing or CDR1 significantly inhibited metastases was sufficient to promote migration...
Abstract This paper closely examines Thucydides’ presentation of three naval battles fought in the Corinthian Gulf and battle Sybota off north-west Greece, order to show how his version action does not just stress pervasive impression Athenian dominance downplay Peloponnesian performance, but extends characterising fleet a surprisingly negative way. In first he claims that they were ignorant local weather patterns, second underwater hazards, after third ‘The Corinthians believed victors if...
<div>Abstract<p>Lung squamous carcinoma (LUSC) is a highly metastatic disease with poor prognosis. Using an integrated screening approach, we found that miR-671-5p reduces LUSC metastasis by inhibiting circular RNA (circRNA), CDR1as. Although the putative function of circRNA through miRNA sponging, more potently silenced axis CDR1as and its antisense transcript, cerebellar degeneration related protein 1 (CDR1). Silencing or CDR1 significantly inhibited metastases was sufficient...
<div>Abstract<p>Lung squamous carcinoma (LUSC) is a highly metastatic disease with poor prognosis. Using an integrated screening approach, we found that miR-671-5p reduces LUSC metastasis by inhibiting circular RNA (circRNA), CDR1as. Although the putative function of circRNA through miRNA sponging, more potently silenced axis CDR1as and its antisense transcript, cerebellar degeneration related protein 1 (CDR1). Silencing or CDR1 significantly inhibited metastases was sufficient...
<p>Time lapse imaging of RUSH assay in SK-MES-1-EV cells transfected with Ii-Str_ssSBP-EGFP (green) and Golgi marker mApple-SiT (magenta) beginning 5 min after biotin addition.</p>
<p>Candidate target genes for miR-671-5p, values based on median expression of miR-671-5p and candidate genes.</p>
<p>Expression of miRs in SK-MES-1 and LN1 measured by Nanostring with normalized counts greater than 5 at least one the two compared samples.</p>
<p>Supplementary Methods, Figures, and Tables</p>
<p>Time lapse imaging of RUSH assay in SK-MES-1-CDR1 cells transfected with Ii-Str_ssSBP-EGFP (green) and Golgi marker mApple-SiT (magenta) beginning 5 min after biotin addition.</p>
<p>Sequences of shRs and RT-qPCR primers.</p>
<p>Gene ontology terms associated with genes elevated in High CDR1-Low miR-671-5p tumors compared to Low CDR1-High miR-671 LUSC TCGA.</p>
<p>Genes differentially expressed in LUSC TCGA High CDR1-Low miR-671-5p tumors compared to Low CDR1-High miR-671 tumors.</p>
<p>miRs individually associated with survival in LUSC TCGA based on median expression of miR genes. FDR values were calculated considering the whole set genes tested (N=387).</p>
<p>Supplementary Methods, Figures, and Tables</p>
<p>Candidate target genes for miR-671-5p, values based on median expression of miR-671-5p and candidate genes.</p>
<p>Gene ontology terms associated with genes elevated in High CDR1-Low miR-671-5p tumors compared to Low CDR1-High miR-671 LUSC TCGA.</p>
<p>Sequences of shRs and RT-qPCR primers.</p>
<p>Time lapse imaging of RUSH assay in SK-MES-1-CDR1 cells transfected with Ii-Str_ssSBP-EGFP (green) and Golgi marker mApple-SiT (magenta) beginning 5 min after biotin addition.</p>
<p>Genes differentially expressed in LUSC TCGA High CDR1-Low miR-671-5p tumors compared to Low CDR1-High miR-671 tumors.</p>
<p>miRs individually associated with survival in LUSC TCGA based on median expression of miR genes. FDR values were calculated considering the whole set genes tested (N=387).</p>
<p>Expression of miRs in SK-MES-1 and LN1 measured by Nanostring with normalized counts greater than 5 at least one the two compared samples.</p>
<p>Time lapse imaging of RUSH assay in SK-MES-1-EV cells transfected with Ii-Str_ssSBP-EGFP (green) and Golgi marker mApple-SiT (magenta) beginning 5 min after biotin addition.</p>