A5043554741- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Adenosine and Purinergic Signaling
- ATP Synthase and ATPases Research
- Genomics and Chromatin Dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroendocrine regulation and behavior
- Epigenetics and DNA Methylation
- Inhalation and Respiratory Drug Delivery
- Neurogenesis and neuroplasticity mechanisms
- Evolutionary Psychology and Human Behavior
- Anthropological Studies and Insights
- Ferroptosis and cancer prognosis
- Virus-based gene therapy research
- Diabetes Management and Education
- Tryptophan and brain disorders
- Brain Metastases and Treatment
- Chinese history and philosophy
University of North Carolina at Chapel Hill
2017-2024
UNC Lineberger Comprehensive Cancer Center
2020
Carolina Institute for NanoMedicine
2019
University of Nebraska Medical Center
2013-2017
University of Nebraska at Omaha
2015
Harvard University
2014
Traumatic brain injury (TBI) is an important health concern and effective treatment strategies remain elusive. Understanding the complex multicellular response to TBI may provide new avenues for intervention. In context of TBI, cell-cell communication critical. One relatively unexplored form in extracellular vesicles (EVs). These membrane-bound can carry many different types cargo between cells. Recently, miRNA EVs have been shown mediate neuroinflammation neuronal injury. To explore role...
The ability to precisely upregulate genes in inflamed brain holds great therapeutic promise. Here we report a novel class of vectors, genetically modified macrophages that carry reporter and neural cells. Systemic administration transfected ex vivo with plasmid DNA (pDNA) encoding potent antioxidant enzyme, catalase, produced month-long expression levels catalase the resulting three-fold reductions inflammation complete neuroprotection mouse models Parkinson's disease (PD). This resulted...
Extracellular vesicles (EVs) are promising natural nanocarriers for delivery of various types therapeutics. Earlier engineered EV-based formulations neurodegenerative diseases and cancer reported. Herein, the use macrophage-derived EVs brain a soluble lysosomal enzyme tripeptidyl peptidase-1, TPP1, to treat storage disorder, Neuronal Ceroid Lipofuscinoses 2 (CLN2) or Batten disease, is investigated. TPP1 loaded into using two methods: i) transfection parental EV-producing macrophages with...
Lung cancer is the leading cause of cancer-related deaths worldwide, and lung squamous carcinomas (LUSC) represent about 30% cases. Molecular aberrations in adenocarcinomas have allowed for effective targeted treatments, but corresponding therapeutic advances LUSC not materialized. However, immune checkpoint inhibitors sub-populations patients led to exciting responses. Using computational analyses The Cancer Genome Atlas, we identified a subset tumors characterized by dense infiltration...
Abstract Metastatic non-small cell lung cancer (NSCLC) remains largely incurable and the prognosis is extremely poor once it spreads to brain. In particular, in patients with brain metastases, blood barrier (BBB) a significant obstacle for biodistribution of antitumor drugs immune cells. Here we report that chimeric antigen receptor (CAR) T cells targeting B7-H3 (B7-H3.CAR) exhibit activity vitro against tumor lines organoids, vivo xenotransplant models orthotopic metastatic NSCLC. The...
Abstract Lymph node (LN) metastases correspond with a worse prognosis in nearly all cancers, yet the occurrence of cancer spreading from LNs remains controversial. Additionally, mechanisms explaining how cancers survive and exit are largely unknown. Here, we show that breast patients frequently have LN closely resemble distant metastases. In addition, using microsurgical model, metastasis development dissemination is regulated by expression chromatin modifier, histone deacetylase 11...
CpG oligodeoxynucleotides are potent toll-like receptor (TLR) 9 agonists and have shown promise as anticancer agents in preclinical studies clinical trials. Binding of to TLR9 initiates a cascade innate adaptive immune responses, beginning with activation dendritic cells resulting range secondary effects that include the secretion pro-inflammatory cytokines, natural killer cells, expansion T cell populations. Recent literature suggests local delivery tumors results superior antitumor...
Oxytocin (OXT) is an important neurohypophyseal hormone that influences wide spectrum of reproductive and social processes. Eutherian mammals possess a highly conserved sequence OXT (Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly). However, in this study, we sequenced the coding region for 22 species covering all New World monkeys (NWM) genera clades, characterize five variants, including consensus mammalian Leu8-OXT, major variant Pro8-OXT, three previously unreported variants: Ala8-OXT, Thr8-OXT,...
Traumatic brain injury (TBI) produces profound and lasting neuroinflammation that has both beneficial detrimental effects. Recent evidence implicated microRNAs (miRNAs) in the regulation of inflammation periphery CNS. We examined expression associated miRNAs context TBI using a mouse controlled cortical impact (CCI) model found increased levels miR-21, miR-223 miR-155 hippocampus after CCI. The was elevated 9-fold CCI, an increase confirmed by situ hybridization (ISH). Interestingly, largely...
Extracellular vesicles (EVs) are cell-derived nanoparticles that facilitate transport of proteins, lipids, and genetic material, playing important roles in intracellular communication. They have remarkable potential as non-toxic non-immunogenic nanocarriers for drug delivery to unreachable organs tissues, particular, the central nervous system (CNS). Herein, we developed a novel platform based on macrophage-derived EVs treat Parkinson disease (PD). Specifically, evaluated therapeutic...
Abstract Lung squamous carcinoma (LUSC) is a highly metastatic disease with poor prognosis. Using an integrated screening approach, we found that miR-671-5p reduces LUSC metastasis by inhibiting circular RNA (circRNA), CDR1as. Although the putative function of circRNA through miRNA sponging, more potently silenced axis CDR1as and its antisense transcript, cerebellar degeneration related protein 1 (CDR1). Silencing or CDR1 significantly inhibited metastases was sufficient to promote migration...
Regulation of cellular proliferation and differentiation during brain development results from processes requiring several regulatory networks to function in synchrony. MicroRNAs are part this system. Although many microRNAs evolutionarily conserved, recent evolution such molecules can enable the acquisition new means attaining specialized functions. Here we identify report novel expression functions a human higher primate-specific microRNA, miR-1290, neurons. Using fetal-derived neural...
Abstract Extracellular vesicles (EVs) are cell-derived nanoparticles that facilitate transport of proteins, lipids and genetic material playing important roles in intracellular communication. They have a remarkable potential as non-toxic non-immunogenic nanocarriers for drug delivery to unreachable organs tissues, particular, the central nervous system (CNS). Herein, we developed novel platform based on macrophage derived EVs treat Parkinson’s disease (PD). Specifically, evaluated...
This collection of essays opens a critical examination compassionate acts responding to social suffering in the intensely complex moral context rapidly changing and globalizing China. Jeanne Shea describes self-compassion among older women China as post-revolutionary response opportunities resistance consumerism. Khun Eng Kuah-Pearce's essay frames Buddhist organizations NGOs shows compassion being mobilized its spiritual-philanthropic, not political. The next three papers illuminate...
Abstract Our group recently discovered that the circular RNA (circRNA), CDR1as, promotes lung cancer metastasis in part through stabilization of coding gene, CDR1. The purpose this study was to identify intramolecular secondary structures CDR1as and determine whether there is a functional relationship between structure non-small cell (NSCLC) metastasis. We employed chemical probing approach selective 2’- hydroxyl acylation analyzed by primer extension mutational profiling (SHAPE-MaP)...
Abstract Oncogenic mutations in the KRAS gene are well-established drivers of cancer. Promising preclinical strategies including RNA interference (RNAi) have been developed to target oncogenic function, yet a clinically effective therapy directly remains be achieved. While genetic knockdown mutant (mKRAS) with RNAi is one promising approach, current methods not mutant-selective, and thus they also decrease normal levels, raising concerns about cell toxicity. Here, we describe custom short...
<div>Abstract<p>Lung squamous carcinoma (LUSC) is a highly metastatic disease with poor prognosis. Using an integrated screening approach, we found that miR-671-5p reduces LUSC metastasis by inhibiting circular RNA (circRNA), CDR1as. Although the putative function of circRNA through miRNA sponging, more potently silenced axis CDR1as and its antisense transcript, cerebellar degeneration related protein 1 (CDR1). Silencing or CDR1 significantly inhibited metastases was sufficient...
<div>Abstract<p>Lung squamous carcinoma (LUSC) is a highly metastatic disease with poor prognosis. Using an integrated screening approach, we found that miR-671-5p reduces LUSC metastasis by inhibiting circular RNA (circRNA), CDR1as. Although the putative function of circRNA through miRNA sponging, more potently silenced axis CDR1as and its antisense transcript, cerebellar degeneration related protein 1 (CDR1). Silencing or CDR1 significantly inhibited metastases was sufficient...
<p>Time lapse imaging of RUSH assay in SK-MES-1-EV cells transfected with Ii-Str_ssSBP-EGFP (green) and Golgi marker mApple-SiT (magenta) beginning 5 min after biotin addition.</p>
<p>Candidate target genes for miR-671-5p, values based on median expression of miR-671-5p and candidate genes.</p>