A5024471814- RNA Research and Splicing
- Lipid metabolism and biosynthesis
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
- Nuclear Structure and Function
- Cell Adhesion Molecules Research
- Congenital Heart Disease Studies
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Metabolomics and Mass Spectrometry Studies
- RNA Interference and Gene Delivery
- Cell Image Analysis Techniques
- MicroRNA in disease regulation
- Amyotrophic Lateral Sclerosis Research
- Viral Infections and Immunology Research
- Alzheimer's disease research and treatments
- Parvovirus B19 Infection Studies
- Circular RNAs in diseases
- Single-cell and spatial transcriptomics
- Force Microscopy Techniques and Applications
Stony Brook University
2025
University of North Carolina at Chapel Hill
2018-2024
McGill University Health Centre
2016-2024
University of North Carolina Health Care
2022-2023
Hôpital Marie Lannelongue
2019-2022
Département Environnement et Agronomie
2022
Université Paris-Saclay
2022
AstraZeneca (United Kingdom)
2020
Université Paris-Sud
2019
Eunice Kennedy Shriver National Institute of Child Health and Human Development
1996-2018
We have prepared a conjugate of epidermal growth factor (EGF) and ferritin that retains substantial binding affinity for cell receptors is biologically active. Glutaraldehyde-activated EGF was covalently linked to produce contained in 1:1 molar ratio. The separated from free by chromatography using antibodies EGF. Monolayers human epithelioid carcinoma cells (A-431) were incubated with EGF:ferritin at 4 degrees C processed transmission electron microscopy. Under these conditions,...
Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between makes it challenging to replicate key findings integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC deeply characterized their genetic properties using whole genome sequencing, genomic stability upon CRISPR-Cas9-based gene editing, including differentiation commonly used types. These studies...
The E4 variant of APOE strongly predisposes individuals to late-onset Alzheimer's disease. We demonstrate that in response lipogenesis, apolipoprotein E (APOE) astrocytes can avoid translocation into the endoplasmic reticulum (ER) lumen and traffic lipid droplets (LDs) via membrane bridges at ER-LD contacts. knockdown promotes fewer, larger LDs after a fatty acid pulse, which contain more unsaturated triglyceride pulse-chase. This LD size phenotype was rescued by chimeric targets only LDs....
Background: Adults with congenital heart disease (CHD) are exposed to increasing amounts of low-dose ionizing radiation (LDIR) from cardiac procedures. Cancer prevalence in this population is higher than the general population. This study estimates association between LDIR exposure procedures and incident cancer adult patients CHD. Methods: The derived Quebec Congenital Heart Disease Database. We measured cumulative numbers LDIR-related for each patient until 1 year before time diagnosis or...
Posttranscriptional regulation of genes mammalian iron metabolism is mediated by the interaction regulatory proteins (IRPs) with RNA stem-loop sequence elements known as iron-responsive (IREs). There are two identified IRPs, IRP1 and IRP2, each which binds consensus IREs present in eukaryotic transcripts equal affinity. Site-directed mutagenesis IRP2 reveals that, although binding affinities for indistinguishable, contributions arginine residues active-site cleft to affinity different sites....
Discoveries spanning several decades have pointed to vital membrane lipid trafficking pathways involving both vesicular and non-vesicular carriers. But the relative contributions for distinct delivery in cell growth organelle biogenesis continue be a puzzle. This is because lipids flow from many sources across paths via transport vesicles, transfer proteins, dynamic interactions between organelles at contact sites. forum presents our latest understanding, appreciation, queries regarding...
Cell and organelle shape are driven by diverse genetic environmental factors thus accurate quantification of cellular morphology is essential to experimental cell biology. Autoencoders a popular tool for unsupervised biological image analysis because they learn low-dimensional representation that maps images feature vectors generate semantically meaningful embedding space morphological variation. The learned can also be used clustering, dimensionality reduction, outlier detection, supervised...
Membrane contact sites (MCSs) are of close apposition between two organelles used to exchange ions, lipids, and information. Cells respond changing environmental or developmental conditions by modulating the number, extent, duration MCSs. Because their small size dynamic nature, tools study dynamics MCSs in live cells have been limited. Dimerization-dependent fluorescent proteins (ddFPs) targeted organelle membranes an ideal tool for studying MCS because they reversibly interact fluoresce...
Parvoviruses are small, nonenveloped, single-stranded DNA viruses which replicate in the nucleus of host cell. We have previously found that early during infection parvovirus minute virus mice (MVM) causes transient disruptions nuclear envelope (NE). now investigated mechanism used by MVM to disrupt NE. Here we show viral phospholipase A2, only known enzymatic domain on capsid, is not involved causing NE disruption. Instead, utilizes cell caspases, proteases breakdown apoptosis, facilitate...
Disassembly of the nuclear lamina is essential in mitosis and apoptosis requiring multiple coordinated enzymatic activities nucleus cytoplasm. Activation coordination different poorly understood moreover complicated as some factors translocate between cytoplasm preparatory phases. Here we used ability parvoviruses to induce membrane breakdown understand triggers key mitotic enzymes. Nuclear envelope disintegration was shown upon infection, microinjection but also their application...
The postantibiotic effect (PAE) is the persistent suppression of microbial growth following removal antimicrobial therapy. In general, antibiotics that generate a PAE are dosed less frequently, and thus, has important implications for dosing regimens. PAEs can arise through several mechanisms, including extended occupancy drug target elimination, correlation between drug-target residence time provides insight into vulnerability. To assess vulnerability Escherichia coli leucyl-tRNA synthetase...
The post-antibiotic effect (PAE) is the delay in bacterial regrowth following antibiotic removal. It has important implications for dosing regimens since drugs that have extended activity their elimination can be dosed less frequently, widening therapeutic window. While PAE been associated with target vulnerability and rate of turnover, little known about genetic components modulate PAE. Here, we developed a high-throughput assay to screen Escherichia coli Keio collection ∼4000 deletion...
The parvovirus Minute virus of mice (MVM) is a small DNA that replicates in the nucleus its host cells. However, very little known about mechanisms underlying parvovirus' nuclear import. Recently, it was found microinjection MVM into cytoplasm Xenopus oocytes causes damage to envelope (NE), suggesting nuclear-import mechanism involves disruption NE and import through resulting breaks. Here, fluorescence microscopy electron were used examine effect on host-cell structure during infection...
Abstract Fluorescent proteins and vital dyes are invaluable tools for studying dynamic processes within living cells. However, the ability to distinguish more than a few different fluorescent reporters in single sample is limited by spectral overlap of available fluorophores. Here, we present protocol imaging live cells labeled with six fluorophores simultaneously. A confocal microscope detector used acquire images, linear unmixing algorithms applied identify each pixel image. We describe...
The heterogeneity of individual cells in a tissue has been well characterized, largely using ex vivo approaches that do not permit longitudinal assessments the same over long periods time. We demonstrate potentially novel application adaptive optics fluorescence microscopy to visualize and track situ mosaicism retinal pigment epithelial (RPE) directly human eye. After short, dynamic period during which RPE take up i.v.-administered indocyanine green (ICG) dye, we observed remarkably stable...