A5041040082- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and biosynthesis
- Adipose Tissue and Metabolism
- Cholesterol and Lipid Metabolism
- Drug Transport and Resistance Mechanisms
- Diet, Metabolism, and Disease
- Eicosanoids and Hypertension Pharmacology
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Cancer, Lipids, and Metabolism
- Neurobiology and Insect Physiology Research
- Microbial Metabolic Engineering and Bioproduction
- Insect Resistance and Genetics
- Muscle metabolism and nutrition
- Fatty Acid Research and Health
- Insect-Plant Interactions and Control
- Pancreatic function and diabetes
- Lipoproteins and Cardiovascular Health
- Arsenic contamination and mitigation
- Nuclear Structure and Function
- Research in Cotton Cultivation
- Metabolism, Diabetes, and Cancer
- Adipokines, Inflammation, and Metabolic Diseases
University of North Carolina at Chapel Hill
2013-2023
Endocrinology Research Center
2014
Indiana University School of Medicine
2013
Medical University of South Carolina
1998-2006
Regulation of fatty acid (FA) metabolism is central to adipocyte dysfunction during diet-induced obesity (DIO). Long-chain acyl-CoA synthetase-4 (ACSL4) has been hypothesized modulate the metabolic fates polyunsaturated FA (PUFA), including arachidonic (AA), but in vivo actions ACSL4 are unknown. The purpose our studies was determine role regulating obesity-associated dysfunction.We developed a novel mouse model with adipocyte-specific ablation (Ad-KO) using loxP Cre recombinase technology....
Mutations in either of two genes comprising the STSL locus, ATP-binding cassette (ABC)-transporters ABCG5 (encoding sterolin-1) and ABCG8 sterolin-2), result sitosterolemia, a rare autosomal recessive disorder sterol trafficking characterized by increased plasma plant levels. Based upon genetics ABCG5/sterolin-1 ABCG8/sterolin-2 are hypothesized to function as obligate heterodimers. No phenotypic difference has yet been described humans with complete defects or ABCG8. These proteins, based...
Because the signaling eicosanoids, epoxyeicosatrienoic acids (EETs) and HETEs, are esterified to membrane phospholipids, we asked which long-chain acyl-CoA synthetase (ACSL) isoforms would activate these molecules whether apparent FA substrate preferences of each ACSL isoform might differ depending on it was assayed in mammalian cell membranes or as a purified bacterial recombinant protein. We found that all five were able use EETs HETEs substrates showed by LC-MS/MS ACSLs produce EET-CoAs....
Abstract Background The molecular mechanisms that regulate the entry of dietary sterols into body and their removal via hepatobiliary secretion are now beginning to be defined. These processes specifically disrupted in rare autosomal recessive disease, Sitosterolemia (MIM 210250). Mutations either, but not both, two genes ABCG5 or ABCG8 , comprising STSL locus, known cause this disease protein products proposed function as heterodimers. Under normal circumstances cholesterol, non-cholesterol...
The metabolic syndrome, a cluster of derangements that include obesity, glucose intolerance, dyslipidemia and hypertension, is major risk factor for cardiovascular disease. Insulin resistance has been proposed to be the common feature links obesity but mechanism remains obscure. Although excess content triacylglycerol in muscle liver highly associated with insulin these tissues, itself not causal merely marker. Thus, attention turned accumulation cellular lipids known have signaling roles....
Glycerol-3-phosphate acyltransferase (GPAT) activity is highly induced in obese individuals with insulin resistance, suggesting a correlation between GPAT function, triacylglycerol accumulation, and resistance. We asked whether microsomal GPAT4, an isoform regulated by insulin, might contribute to the development of hepatic Compared control mice fed high fat diet, Gpat4(-/-) were more glucose tolerant protected from Overexpression GPAT4 mouse hepatocytes impaired insulin-suppressed...
Elevated plant sterol accumulation has been reported in the spontaneously hypertensive rat (SHR), stroke-prone (SHRSP) and Wistar-Kyoto (WKY) rat. Additionally, a blood pressure quantitative trait locus (QTL) mapped to chromosome 6 New Zealand genetically strain (GH rat). ABCG5 ABCG8 (encoding sterolin-1 sterolin-2 respectively) have shown be responsible for causing sitosterolemia humans. These genes are organized head-to-head configuration at STSL on human 2p21. To investigate whether...