Breast cancer is a potentially fatal malignancy in females despite the improvement therapeutic techniques. The identification of novel molecular signatures needed for earlier detection, monitoring effects treatment, and predicting prognosis. We have previously used microarray analysis to identify differentially expressed genes aggressive breast tumors. purpose present study was investigate prognostic value candidate biomarkers CCNB2, ASPM, CDCA7, KIAA0101, SLC27A2 cancer. expression levels...

Abstract Cancer drug development has been riddled with high attrition rates, in part, due to poor reproducibility of preclinical models for discovery. Poor experimental design and lack scientific transparency may cause biases that turn affect data quality, robustness reproducibility. Here, we pinpoint sources variability conventional 2D cell-based cancer screens determine the effect confounders on cell viability MCF7 HCC38 breast lines treated platinum agents (cisplatin carboplatin) a...

The BIRC5 gene encodes for the Survivin protein, which is a member of inhibitor apoptosis family. found in humans during fetal development, but generally not adult cells thereafter. Previous studies have shown that abundant most cancer cells, thereby making it promising target anti-cancer drugs and potential prognostic tool.To assess genetic alterations mutations as well co-expression with other genes, genomic transcriptomic data were downloaded via cBioPortal approximately 9000 samples from...

Deregulation of key cellular pathways is fundamental for the survival and expansion neoplastic cells. In cancer, regulation gene transcription can be mediated in a variety ways. The purpose this study was to assess impact dosage on expression patterns effect other mechanisms transcriptional levels, associate these genomic changes with clinicopathologic parameters.We screened 97 invasive diploid breast tumors DNA copy number alterations levels using array comparative hybridization...

Genetic and epigenetic (DNA methylation, histone modifications, microRNA expression) crosstalk promotes inactivation of tumor suppressor genes or activation oncogenes by gene loss/hypermethylation duplications/hypomethylation, respectively. The 8p11-p12 chromosomal region is a hotspot for genomic aberrations (chromosomal rearrangements, amplifications deletions) in several cancer forms, including breast carcinoma where amplification has been associated with increased proliferation rates...

Abstract Triple-negative breast cancer (TNBC) is associated with poor prognosis and limited treatment options due to the lack of important receptors (estrogen receptor [ER], progesterone [PR], human epidermal growth factor 2 [HER2]) used for targeted therapy. However, high-throughput in vitro drug screening cell lines a powerful tool identifying effective drugs disease. Here, we determine intrinsic chemosensitivity TNBC proteasome inhibitors (PIs), thereby potentially potent 2-drug...

The deregulation of key cellular pathways is fundamental for the survival and expansion neoplastic cells, which in turn can have a detrimental effect on patient outcome. To develop effective individualized cancer therapies, we need to better understanding are perturbed genetically defined subgroup patients. Here, validate prognostic value 13‐marker signature independent gene expression microarray datasets ( n = 1,141) immunohistochemistry with full‐faced FFPE samples 71). predictive...

In cancer radiotherapy, knowledge of normal tissue responses and toxicity risks is essential in order to deliver the highest possible absorbed dose tumor while maintaining exposure at non-critical levels. However, few studies have investigated vivo after (211)At administration. identify molecular biomarkers ionizing radiation exposure, we genome-wide transcriptional (very) low mean doses from mouse tissues.Female BALB/c nude mice were intravenously injected with 1.7 kBq killed 1 h, 6 or 7...

About one-third of oestrogen receptor alpha-positive breast cancer patients treated with tamoxifen relapse. Here we identify the nuclear retinoic acid alpha as a marker resistance. Using quantitative mass spectrometry-based proteomics, show that protein networks and levels differ in tamoxifen-sensitive (MCF7) tamoxifen-resistant (LCC2) cell line. High intratumoural also correlate reduced relapse-free survival adjuvant solely. A similar expression pattern is seen comparable independent...

Abstract Background Ovarian cancer is the main cause of gynecological cancer-associated death. However, 5-year survival rates differ dramatically between five ovarian carcinoma histotypes. Therefore, we need to have a better understanding mechanisms that promote histotype-specific carcinogenesis and identify novel prognostic biomarkers. Methods Here, evaluated role 29 genes for early-stage (I II) carcinomas ( n = 206) using immunohistochemistry (IHC). Results We provide evidence aberrant...

Breast cancer (BC) histological and molecular classifications significantly improved the treatment strategy prognosis. Inhibitor of apoptosis BIRC5/survivin is often overexpressed in cancers, however, indications its importance BC are inconsistent. We integrate BIRC5 protein mRNA measures with clinical associates long-term outcome three independent cohorts Protein levels were measured primary lysates 845 patients West Swedish cohort (VGR-BC) linked to 5- 27-years survival. The results...

Progression to metastasis is the proximal cause of most cancer-related mortality. Yet much remains be understood about what determines spread tumor cells. This paper describes a novel pathway in breast cancer that regulates epithelial-to-mesenchymal transition (EMT), motility, and invasiveness. We identify two transcription factors, nuclear factor 1-C2 (NF1-C2) Forkhead box F1 (FoxF1), downstream prolactin/nuclear Janus-activated kinase 2, with opposite effects on these processes. show...

Squamous cell carcinoma of the oral cavity (OSCC) is a common cancer form with relatively low 5-year survival rates, due partially to late detection and lack complementary molecular markers as targets for treatment. Molecular profiling head neck has revealed biological similarities basal-like breast lung carcinoma. Recently, we showed that 16 genes were consistently altered in invasive tumors displaying varying degrees aggressiveness. To extend our findings from another type similar...

Previously, we performed analysis of gene expression in 46 axillary lymph node negative tumors and identified molecular signatures that resulted different clinical outcomes. The aim this study was to determine the correlation γ-glutamyl hydrolase (GGH), fatty acid amide (FAAH), Pirin (PIR) TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65 kDa (TAF5L), selected from signatures, with outcomes as well classical clinicopathological characteristics primary...

Early-stage (I and II) ovarian carcinoma patients generally have good prognosis. Yet, some die earlier than expected. Thus, it is important to stratify early-stage into risk groups identify those in need of more aggressive treatment regimens. The prognostic value 29 histotype-specific biomarkers identified using RNA sequencing was evaluated for clear-cell (CCC), endometrioid (EC) mucinous (MC) carcinomas (n = 112) immunohistochemistry on tissue microarrays. Biomarkers with significance were...

Previous studies have shown that the ADIPOR1, ADORA1, BTG2 and CD46 genes differ significantly between long-term survivors of breast cancer deceased patients, both in levels gene expression DNA copy numbers. The aim this study was to characterize corresponding proteins carcinoma determine their correlation with clinical outcome. Protein evaluated using immunohistochemistry an independent cohort 144 samples represented on tissue microarrays. Fisher's exact test used analyze differences...

Abstract Ovarian cancer is the leading cause of death among patients with gynecological cancers, but biology these tumors still least understood all major human malignancies. In this study, comparative genomic hybridization was used to determine chromosomal alterations in 98 stage III serous papillary adenocarcinomas. The were grouped according survival and main prognostic factors surgical outcome. There imbalances that significantly more common from who died than survived: gains 1q24–qter...

It is of great significance to find better markers correctly distinguish between high-risk and low-risk breast cancer patients since the majority cases are at present being overtreated. 46 tumours from node-negative were studied with gene expression microarrays. A t-test was carried out in order a set genes where might predict clinical outcome. Two classifiers used for evaluation lists, correlation-based classifier Voting Features Interval (VFI) classifier. We then evaluated predictive...

Astatine-211 (211At) is an alpha particle emitting halogen with almost optimal linear energy transfer for creating DNA double-strand breaks and thus proposed radionuclide therapy when bound to tumor-seeking agents. Unbound 211At accumulates in the thyroid gland, concept of basal radiation-induced biological effects tissue is, a high degree, unknown most valuable. Female BALB/c nude mice were intravenously injected 0.064 42 kBq 211At, resulting absorbed doses 0.05 32 Gy gland. Thyroids...

(211)At is a promising therapeutic radionuclide because of the nearly optimal biological effectiveness emitted α-particles. Unbound accumulates in thyroid gland and other vital normal tissues. However, few studies have been performed that assess (211)At-induced normal-tissue damage vivo. Knowledge about extent quality resulting responses various organs offers new venue for reducing risks side effects increasing overall well-being patient during after therapy.Female BALB/c nude mice were...

The aim of this study was to investigate the global gene expression response normal tissues following internal low absorbed dose irradiation 131I.Balb/c mice were intravenously injected with 13 260 kBq 131I and euthanized 24 h after injection. Kidneys, liver, lungs, spleen surgically removed. 0.1 9.7 mGy. Total RNA extracted, Illumina MouseRef-8 Whole-Genome Expression BeadChips (Illumina, Inc., San Diego, California, USA) used compare irradiated that non-irradiated controls....

177Lu-octreotate can be used to treat somatostatin receptor expressing neuroendocrine tumors. It is highly effective in animal models, but clinical studies have so far only demonstrated low cure rates. Hedgehog inhibitors shown therapeutic effect as monotherapy tumor model systems and might one option enhance the efficacy of therapy. The aim this study was determine combination therapy using signaling pathway inhibitor sonidegib. GOT1-bearing BALB/c nude mice were treated with either...

Abstract Mutation analysis is typically performed at the DNA level since most technical approaches are developed for analysis. However, some applications, like transcriptional mutagenesis, RNA editing and gene expression analysis, require Here, we combine reverse transcription digital sequencing to enable low error sequencing. We evaluate yield, reproducibility, dynamic range correction rate seven different conditions using multiplexed assays. The reproducibility vary substantially between...