A5067204984- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Growth Hormone and Insulin-like Growth Factors
- Reproductive Biology and Fertility
- Birth, Development, and Health
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Pancreatic function and diabetes
- Genomic variations and chromosomal abnormalities
- Animal Genetics and Reproduction
- Genetic Syndromes and Imprinting
- Metabolism and Genetic Disorders
- DNA Repair Mechanisms
- Molecular Biology Techniques and Applications
- Renal and related cancers
- Kruppel-like factors research
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
Université Libre de Bruxelles
2002-2021
Roslin Institute
2001
Zimmer Biomet (United Kingdom)
2001
University of Gothenburg
1986-1999
Harvard University
1995-1997
Brigham and Women's Hospital
1997
Istituto Neurologico Mediterraneo
1997
University of Kansas Medical Center
1993-1996
University of California, San Diego
1996
San Francisco VA Medical Center
1996
Abstract Nicotinamide phosphoribosyl transferase (Nampt)/pre-B cell colony-enhancing factor (PBEF)/visfatin is a protein displaying multiple functional properties. Originally described as cytokine-like able to regulate B development, apoptosis, and glucose metabolism, this also plays an important role in NAD biosynthesis. To gain insight into its physiological role, we have generated mouse strain expressing conditional Nampt allele. Lack of expression strongly affects development both T...
In the HXB and BXH recombinant inbred strains derived from spontaneously hypertensive rat normotensive Brown Norway rat, we determined strain distribution patterns of 500 genetic markers to scan rodent genome for quantitative trait loci regulating cardiac mass blood pressure. The spanned approximately 1,139 cM were tested correlations with left ventricular adjusted body weight, systolic, diastolic, mean arterial pressures. marker dopamine 1A receptor (Drd1a) on chromosome 17 showed strongest...
The alpha-fetoprotein (AFP) gene is highly activated in fetal liver but dramatically repressed shortly after birth. mechanisms that underlie AFP transcriptional repression postpartum are not well understood. enhancer, repressor region, and promoter implicated to be involved postnatal repression, the major remains undefined. We previously identified a zinc finger protein ZBTB20. To determine its physiological functions vivo , we have generated hepatocyte-specific ZBTB20 knockout mice by...
Genetic susceptibility to type 2 diabetes involves many genes, most of which are still unknown. The lipid phosphatase SHIP2 is a potent negative regulator insulin signaling and sensitivity in vivo thus good candidate gene. Here we report the presence gene mutations associated with rats humans. R1142C mutation specifically identified Goto-Kakizaki (GK) spontaneously hypertensive rat strains disrupts potential class II ligand for Src homology (SH)-3 domain slightly impairs cell culture. In...
The methods of somatic cell genetics and molecular hybridization were applied to a panel mouse × rat hepatocyte hybrids segregating chromosomes assign the genes coding for two serum proteins, albumin α-fetoprotein (<i>Alb </i>and <i>Afp</i>). DNAs from different with cloned DNA probes showed that all hybrid clones possessing <i>Alb </i>gene expressing it also retained <i>Afp </i>locus, which is not expressed in these hybrids. So </i>genes...
We report characterization of a novel isoform lysyl hydroxylase (lysyl 3, LH3). The cDNA clones encode polypeptide 738 amino acids, including signal peptide. acid sequence has high overall identity with LH1 and LH2, the isoforms characterized earlier. Conserved regions are present in carboxyl-terminal portion also central part molecules. Histidine asparagine residues, which conserved other known to be required for enzymatic activity, isoform. gene LH3 (PLOD3) been assigned human chromosome...
The human type 2 angiotensin II receptor (AT2R) gene was isolated from a genomic DNA library of adult female leukocytes. putative coding region the AT2R is intronless. primary structure deduced nucleotide sequence this contains 363 amino acid residues and highly homologous to sequences rat mouse AT2R. mapped X chromosome using chromosomal assignment analysis with human/rodent somatic cell hybrid panel. Northern blot demonstrated 3.0 kb mRNA in fetal lung kidney lung. These data probes should...
Androgens and their nuclear receptor regulate genes necessary for development of the male phenotype, a process that is thought to be modulated by extracellular androgen carrier proteins. Two these proteins, testicular androgen-binding protein (ABP) plasma sex hormone-binding globulin (SHBG), are encoded same gene, but differ in glycosylation possibly amino acid sequence. To investigate ABP-SHBG gene expression fetal rat liver, we analyzed RNA transcripts expressed protein. These studies...
In this report, we describe the isolation, molecular cloning, and characterization of a new member prolactin (PRL)-growth hormone (GH) family expressed in rat decidual tissue. A 29-kDa protein was isolated from medium conditioned by explants. The possessed an affinity for concanavalin cross-reactivity with antibodies to two placental proteins, PRL-like protein-B (PLP-B) PLP-C human PRL. NH2-terminal sequencing indicated that it shared significant sequence identity NH2 terminus PLP-C. termed...
The spontaneously hypertensive rat (SHR) is the most widely studied animal model of essential hypertension. Despite > 30 yr research, primary genetic lesions responsible for hypertension in SHR remain undefined. In this report, we describe construction and hemodynamic characterization a congenic strain (SHR-Lx) that carries defined segment chromosome 8 from normotensive Brown-Norway rats (BN-Lx strain). Transfer BN-Lx onto background resulted substantial reductions systolic diastolic blood...
Sex differences in gonadal function are driven by either cyclical (females) or tonic (males) hypothalamic GnRH1 release and, subsequently, gonadotrophin (LH and FSH) secretion from the pituitary. This sex difference seems to depend on perinatal actions of hormones hypothalamus. We used α-fetoprotein (AFP) knockout mice (Afp−/−) study mechanisms which estrogens affect sexual differentiation system. Afp−/− lack protective AFP against circulating during embryonic development, leading...