A5061047762- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Ion Transport and Channel Regulation
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Protein Hydrolysis and Bioactive Peptides
- Endoplasmic Reticulum Stress and Disease
- Growth Hormone and Insulin-like Growth Factors
- Pancreatic function and diabetes
- Studies on Chitinases and Chitosanases
- Blood groups and transfusion
- Nutritional Studies and Diet
- Metabolism, Diabetes, and Cancer
- Microbial Metabolites in Food Biotechnology
- Genetic Syndromes and Imprinting
- Diet and metabolism studies
- Probiotics and Fermented Foods
- Metabolomics and Mass Spectrometry Studies
- Muscle metabolism and nutrition
- Genetic factors in colorectal cancer
- Microbial Natural Products and Biosynthesis
- Ubiquitin and proteasome pathways
University of Liège
2001-2014
Gembloux Agro-Bio Tech
2010-2014
Université Libre de Bruxelles
2008
A novel serine protease gene, SBcas3.3, was identified by functional screening of a forest-soil metagenomic library on agar plates supplemented with AZCL-casein. Overproduction in Escherichia coli revealed that the enzyme is produced as 770-amino-acid precursor which processed to mature ~55 kDa. The latter purified affinity chromatography for characterization azocasein substrate. proved be an alkaline showing maximal activity between pH 9 and 10 at 50°C. Treatment chelating agent...
A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence gene cluster analysis showed wide diversity identified enzymes gave an idea microbial populations present during sample collection period. Lastly, endo-β-1,4-glucanase having less than 50% identity to sequences known cellulases purified partially characterized, showing...