A5005306560- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Plant Pathogenic Bacteria Studies
- Enzyme Catalysis and Immobilization
- Bacterial biofilms and quorum sensing
- Mycorrhizal Fungi and Plant Interactions
- Fungal and yeast genetics research
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Production and Characterization
- Plant Parasitism and Resistance
- Studies on Chitinases and Chitosanases
- Plant Virus Research Studies
- Mycotoxins in Agriculture and Food
- Metabolomics and Mass Spectrometry Studies
- Agricultural pest management studies
- Mass Spectrometry Techniques and Applications
- Phytase and its Applications
- Polysaccharides and Plant Cell Walls
- Fungal Biology and Applications
- Nitrogen and Sulfur Effects on Brassica
- Transgenic Plants and Applications
- Bacterial Genetics and Biotechnology
- Plant responses to water stress
Gembloux Agro-Bio Tech
2014-2024
University of Liège
2014-2024
AgroBio
2017
Bacillus velezensis is considered as model species for plant-associated bacilli providing benefits to its host such protection against phytopathogens. This mainly due the potential secrete a wide range of secondary metabolites with specific and complementary bioactivities. metabolite arsenal has been quite well defined genetically chemically but much remains be explored regarding how it expressed under natural conditions notably can modulated upon interspecies interactions in competitive...
Most isolates belonging to the Bacillus amyloliquefaciens subsp. plantarum clade retain potential produce a vast array of structurally diverse antimicrobial compounds that largely contribute their efficacy as biocontrol agents against numerous plant fungal pathogens. In context, role cyclic lipopeptides (CLPs) has been well-documented but still little is known about impact interactions with other soil-inhabiting microbes on expression these molecules. this work, we wanted investigate...
Plant-associated Bacillus velezensis and Pseudomonas spp. represent excellent model species as strong producers of bioactive metabolites involved in phytopathogen inhibition the elicitation plant immunity. However, ecological role these during microbial interspecies interactions way their expression may be modulated under naturally competitive soil conditions has been poorly investigated.
A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence gene cluster analysis showed wide diversity identified enzymes gave an idea microbial populations present during sample collection period. Lastly, endo-β-1,4-glucanase having less than 50% identity to sequences known cellulases purified partially characterized, showing...
Highlights•B. velezensis colonizes the entire mycelial network more efficiently than roots•Bacillus uses fungal hyphae as highways for soil invasion and to colonize new plant•The lipopeptide surfactin plays key roles in chemical ecology of interaction•The microbial partnership enhances systemic resistance tomato against BotrytisSummaryArbuscular mycorrhizal (AM) fungi (e.g., Rhizophagus species) recruit specific bacterial species their hyphosphere. However, interplay mutual benefit this...
In the prospect of novel potential biocontrol agents, a new strain BDI-IS1 belonging to recently described Bacillus nakamurai was selected for its strong in vitro antimicrobial activities against range bacterial and fungal phytopathogens. Genome mining coupled with metabolomics revealed that produces multiple non-ribosomal secondary metabolites including surfactin, iturin A, bacillaene, bacillibactin bacilysin, together some ribosomally-synthesized post-translationally modified peptides...
In recent years, the non-conventional model yeast species Yarrowia lipolytica has received much attention because it is a useful cell factory for producing recombinant proteins. this species, expression vectors involving LIP2 and POX2 promoters have been developed used successfully protein production at yields similar to or even higher than those of other factories, such as Pichia pastoris. However, processes these can be difficult manage, especially if carried out large scales in fed-batch...
Crown gall disease caused by Agrobacterium tumefaciens is considered to be the main bacterial threat of stone fruit plants in Mediterranean countries. In a previous study, Bacillus velezensis strain 32a was isolated from Tunisian rhizosphere soil and revealed high antagonistic potential against A. strains. order better characterize activity this important plant pathogen, production secondary metabolites analyzed using liquid chromatography coupled with mass spectrometry. The results...
Abstract The large-scale production of recombinant proteins (rProt) is becoming increasingly economically important. Among the different hosts used for rProt production, yeasts are gaining popularity. so-called non-conventional yeasts, such as methylotrophic Pichia pastoris and dimorphic Yarrowia lipolytica , popular choices due to their favorable characteristics well-established expression systems. Nevertheless, a direct comparison two systems secretion was lacking. This study therefore...
Cocultures have been widely explored for their use in deciphering microbial interaction and its impact on the metabolisms of interacting microorganisms. In this work, we investigate, different liquid coculture conditions, compatibility two microorganisms with potential biocontrol plant diseases: fungus Trichoderma harzianum IHEM5437 bacterium Bacillus velezensis GA1 (a strong antifungal lipopeptide producing strain). While overgrew a rich medium due to production, drastically trend was...
Komagataella phaffii (aka Pichia pastoris) is a yeast able to grow in methanol as the sole carbon and energy source. This substrate converted into formaldehyde, toxic intermediary that can either be assimilated biomass or dissimilated CO2 through enzymes formaldehyde dehydrogenase (FLD) formate dehydrogenase, also producing form of NADH. The dissimilative pathway has been described an detoxifying route, but conclusive evidence not provided for this. In order elucidate this theory, we...
Bacillus velezensis isolates are among the most promising plant-associated beneficial bacteria used as biocontrol agents. However, various aspects of chemical communication between plant and these beneficials, determining root colonization ability, remain poorly described. Here we investigated molecular basis such interkingdom interaction occurring upon contact its host via sensing pectin backbone homogalacturonan (HG). We showed that B. stimulates key developmental traits a dynamic process...
Bacillus velezensis is considered as a model species belonging to the so-called subtilis complex that evolved typically dwell in soil rhizosphere niche and establish an intimate association with plant roots. This bacterium provides protection its natural host against diseases represents one of most promising biocontrol agents. However, molecular basis cross talk this establishes has been poorly investigated. We show here these plant-associated bacteria have polymer-sensing system perceive...
The rhizobacterium Bacillus amyloliquefaciens subsp. plantarum S499 (S499) is particularly efficient in terms of the production cyclic lipopeptides, which are responsible for high level plant disease protection provided by this strain. Sequencing genome has highlighted genetic differences and similarities with closely related B. FZB42 (FZB42). More specifically, a rare 8008 bp plasmid (pS499) harboring rap-phr cassette constitutes major distinctive element between FZB42. By curing plasmid,...
In response to osmotic stress, the yeast Yarrowia lipolytica produces erythritol, a four-carbon sugar alcohol, from erythrose-P, an intermediate of pentose phosphate pathway. Under non-stressing conditions (isotonic environment), produced erythritol is subsequently recycled into erythrose-P that can feed Herein, gene YALI0F01584g was characterized as involved in catabolic Several experimental evidences suggested it encodes erythrulose-1P isomerase converts erythrulose-4P. On basis our...
Abstract Industrially relevant traits of Yarrowia lipolytica , like high growth rate, capacity to grow at cell density or synthesize biomolecules with productivities, strongly rely on sufficient oxygen provision. Although the impact availability (OA) physiology Y. has been already studied, its influence recombinant protein (rProt) synthesis and secretion largely neglected date. With aim fill this gap, a fluorescent reporter (yellow [YFP]) was used herein as proxy follow simultaneously rProt...
Abstract Pichia pastoris is a very popular yeast for recombinant protein production, mainly due to the strong, methanol‐inducible P AOX1 promoter. Methanol induction however poses several drawbacks. One approach improve processes use MutS strains with reduced methanol catabolic ability. Various reports claim that allows higher production levels than Mut+ but scarcely elaborate on reasons differences. In this study, enhanced green fluorescent was used as ‐driven reporter investigation of...
Abstract Arbuscular mycorrhizal (AM) fungi (e.g. Rhizophagus irregularis ) recruit specific bacterial species in their hyphosphere. However, the chemical interplay and mutual benefit of this intricate partnership have not yet been investigated especially as it involves bacteria known strong producers antifungal compounds such Bacillus velezensis . Here, we show that soil dwelling B. migrates along hyphal network AM fungus R. , forming biofilms inducing metabolic fluxes contributes to host...
Abstract Some bacterial species are important members of the rhizosphere microbiome and confer protection to host plant against pathogens. However, our knowledge multitrophic interactions determining ecological fitness these biocontrol bacteria in their highly competitive natural niche is still limited. In this work, we investigated molecular mechanisms underlying between B. velezensis, considered as model plant-associated beneficial Bacillus genus, Pseudomonas a rhizosphere-dwelling...
Crown gall disease caused by Agrobacterium tumefaciens is considered to be the main bacterial threat of stone fruit plants in Mediterranean countries. In a previous study, Bacillus velezensis strain 32a was isolated from Tunisian rhizosphere soil and revealed high antagonistic potential against A. strains. order better characterize activity this important plant pathogen, production secondary metabolites analyzed using liquid chromatography coupled with mass spectrometry. The results...
Arbuscular mycorrhizal (AM) fungi (e.g. Rhizophagus irregularis) recruit specific bacterial species in their hyphosphere. However, the chemical interplay and mutual benefit of this intricate partnership have not been investigated yet, especially as it involves bacteria known strong producers antifungal compounds such Bacillus velezensis. Here, we show that soil dwelling B. velezensis migrates along hyphal network AM fungus R. irregularis, forming biofilms inducing metabolic fluxes...