Lactobacillus sakei is a psychotrophic lactic acid bacterium found naturally on fresh meat and fish. This microorganism widely used in the manufacture of fermented meats has biotechnological potential biopreservation food safety. We have explored 1,884,661-base-pair (bp) circular chromosome strain 23K encoding 1,883 predicted genes. Genome sequencing revealed specialized metabolic repertoire, including purine nucleoside scavenging that may contribute to an ability successfully compete raw...

Yarrowia lipolytica is a biotechnological chassis for the production of range products, such as microbial oils and organic acids. However, it unable to consume xylose, major pentose in lignocellulosic hydrolysates, which are considered preferred carbon source bioprocesses due their low cost, wide abundance high sugar content. Here, we engineered Y. metabolize xylose produce lipids or citric acid. The overexpression reductase xylitol dehydrogenase from Scheffersomyces stipitis were necessary...

Yarrowia lipolytica is a common biotechnological chassis for the production of lipids, which are preferred feedstock fuels and chemicals. To reduce cost microbial lipid production, inexpensive carbon sources must be used, such as lignocellulosic hydrolysates. Unfortunately, materials often contain toxic compounds large amount xylose, cannot used by Y. lipolytica.In this work, we engineered yeast to efficiently use xylose source lipids overexpressing native genes. We further increased content...

Microbial biolipid production has become an important part of making biofuel economically feasible. Genetic engineering been used to improve the ability Yarrowia lipolytica, oleaginous yeast, produce lipids using glucose-based media. However, few studies have examined lipid accumulation by Y. lipolytica's utilize other hexose sugars, and as yet, rate-limiting steps in this process are unidentified. In study, we investigated de novo lipolytica when grown glucose, fructose, sucrose. Three...

The complete 31754 bp genome of bIL170, a virulent bacteriophage Lactococcus lactis belonging to the 936 group, was analysed. Sixty-four ORFs were predicted and function 16 them assigned by significant homology proteins in databases. Three putative homing endonucleases HNH family found early region. An endonuclease with zinc-binding motif identified late cluster, potentially being part same functional module as terminase. structural analysed detail show interesting features among dairy...

ABSTRACT Lactobacillus sakei is a lactic acid bacterium that ubiquitous in the food environment and one of most important constituents commercial meat starter cultures. In this study, vivo expression technology (IVET) was applied to investigate gene L. 23K during fermentation. The IVET vector used (pEH100) contained promoterless transcriptionally fused reporter genes mediating β-glucuronidase activity erythromycin resistance. A genomic library established, clones were subjected fermentation...

Sugar assimilation has been intensively studied in the model yeast S. cerevisiae, and for two decades, it clear that homologous HXT genes, which encode a set of hexose transporters, play central role this process. However, Yarrowia lipolytica, is well-known its biotechnological applications, sugar only poorly understood, even though exhibits peculiar intra-strain differences fructose uptake: some strains (e.g., W29) are known to be slow-growing while others H222) grow rapidly under same...

In recent years, the non-conventional model yeast species Yarrowia lipolytica has received much attention because it is a useful cell factory for producing recombinant proteins. this species, expression vectors involving LIP2 and POX2 promoters have been developed used successfully protein production at yields similar to or even higher than those of other factories, such as Pichia pastoris. However, processes these can be difficult manage, especially if carried out large scales in fed-batch...

In the context of sustainable development, yeast are one class microorganisms foreseen for production oil from diverse renewable feedstocks, in particular those that do not compete with food supply. However, their use bulk production, such as biodiesel, is still cost effective, partly due to possible poor desired substrates or robustness practical bioconversion process. We investigated natural capacity Blastobotrys adeninivorans, a already used biotechnology, store lipids under different...

ABSTRACT The complete nucleotide sequence of the 13-kb plasmid pRV500, isolated from Lactobacillus sakei RV332, was determined. Sequence analysis enabled identification genes coding for a putative type I restriction-modification system, two recombinases integrase family, and region likely involved in replication. structural features this region, comprising ori segment containing 11- 22-bp repeats repA gene initiator protein, indicated that pRV500 belongs to pUCL287 subfamily theta-type...

The development of efficient bioprocesses requires inexpensive and renewable substrates. Molasses, a by-product the sugar industry, contains mostly sucrose, disaccharide composed glucose fructose, both easily absorbed by microorganisms. Yarrowia lipolytica, platform for production various chemicals, can be engineered sucrose utilization heterologous invertase expression, yet problem preferential use over fructose remains, as consumption begins only after depletion what significantly extends...

Abstract Background Alternative sigma factors trigger various adaptive responses. Lactobacillus sakei , a non-sporulating meat-borne bacterium, carries an alternative factor seemingly orthologous to σ H of Bacillus subtilis best known for its contribution the initiation large starvation response ultimately leading sporulation. As role -like has been little studied in bacteria, we investigated function L. . Results Transcription sigH coding was hardly affected by entry into stationary phase...

Although Yarrowia lipolytica is a model yeast for the study of lipid metabolism, its diversity poorly known, as studies generally consider only few standard laboratory strains. To extend our knowledge this biotechnological workhorse, we investigated genomic and phenotypic 56 natural isolates. Y. classified into five clades with no correlation between clade membership geographic or ecological origin. A low genetic (π = 0.0017) pan-genome (6528 genes) barely different from core genome (6315...

ABSTRACT Blastobotrys raffinosifermentans is an ascomycetous yeast with biotechnological applications, recently shown to be oleaginous accumulating lipids under nitrogen limitation. Diacylglycerol acyltransferases (DGATs) act in the lipid storage pathway, last step of triacylglycerol biosynthesis. Two DGAT families are widespread eukaryotes. We first checked that B. strain LS3 possessed both types DGAT, and we then overexpressed native DGAT-encoding genes, DGA1 DGA2, separately or together....