A5088505544- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Microbial Metabolites in Food Biotechnology
- Fungal and yeast genetics research
- Fermentation and Sensory Analysis
- Yeasts and Rust Fungi Studies
- Mineral Processing and Grinding
- Probiotics and Fermented Foods
- Agriculture, Plant Science, Crop Management
- Metabolism, Diabetes, and Cancer
- Geophysical and Geoelectrical Methods
- Geochemistry and Geologic Mapping
- Analytical Chemistry and Chromatography
- Food composition and properties
- Amino Acid Enzymes and Metabolism
- Mycotoxins in Agriculture and Food
- Microbial bioremediation and biosurfactants
- Enzyme Structure and Function
- Plant Pathogens and Fungal Diseases
- Enzyme Production and Characterization
- Nanoparticles: synthesis and applications
- Nutrition and Health Studies
- Chemistry and Chemical Engineering
- Peptidase Inhibition and Analysis
Wroclaw University of Environmental and Life Sciences
2009-2022
Institute of Agricultural and Food Biotechnology
2017
University of Wrocław
2000-2009
University of Silesia in Katowice
2009
Instytut Środowiska Rolniczego i Leśnego Polskiej Akademii Nauk
1995-2005
University of Business in Wrocław
2000
University of Agriculture in Krakow
1994
Wroclaw Medical University
1983
Polish Academy of Sciences
1981
Sugar assimilation has been intensively studied in the model yeast S. cerevisiae, and for two decades, it clear that homologous HXT genes, which encode a set of hexose transporters, play central role this process. However, Yarrowia lipolytica, is well-known its biotechnological applications, sugar only poorly understood, even though exhibits peculiar intra-strain differences fructose uptake: some strains (e.g., W29) are known to be slow-growing while others H222) grow rapidly under same...
The progress of research on silver nanoparticles (AgNPs) has led to their inclusion in many consumer products (chemicals, cosmetics, clothing, water filters, and medical devices) as a biocide. Despite the widespread use AgNPs, biocidal activity is not yet fully understood usually associated with various factors (size, composition, surface, red-ox potential, concentration) and, obviously, specific features microorganisms. There are merely few studies concerning interaction molds AgNPs....
ABSTRACT Yarrowia lipolytica is an early diverging species of the Saccharomycotina subphylum, which recognized as a valuable host for many biotechnological applications exploiting its oleaginous capacities. The 20.5-Mb genome Polish Y. strain A-101 will greatly help decipher genetic basis regulation lipid metabolism.
Yarrowia lipolytica is a non-conventional yeast producing valuable compounds, such as citric acids, from renewable raw materials. This study investigated the impact of citrate synthase overexpression on biosynthesis and isocitric acid in Y. lipolytica. Two transformants A101.1.31 strain (efficient producer), overexpressing CIT1 or CIT2 gene (encoding proteins with activity), were constructed. The results revealed that either these genes enhances activity. Additionally, cit1 knockout was...
The ability of 16 strains
Invertases are important enzymes used in the food industry. Despite many studies on invertase-encoding SUC2 gene expression industrial yeast Yarrowia lipolytica, no biochemical characteristics of this enzyme expressed as heterologous protein have been provided. Here, two isoforms extracellular invertase produced by Y. lipolytica were detected using ion-exchange chromatography. Specific activities 226.45 and 432.66 U/mg for first second isoform, respectively, determined. Basic similar to one...
The goal of the experiment was to verify geoelectrical methods as a tool for detection oil<br>contaminations in soils and monitor their bioremediation process. survey area approximately<br>2000 m2 is situated former military camp Borne Sulinowo NW Poland. covered by<br>fluvioglacial sediments composed sands gravels with first water level at 11 ¸ 12 m depth.<br>Numerous shallow bore-holes were drilled recognize detailed geological structure. The<br>measurements...
Restriction fragment length polymorphism (RFLP) patterns of PCR-amplified ribosomal RNA gene fragments (rDNA) and randomly amplified polymorphic DNA (RAPD) were applied for the analysis 15 brewing 6 related yeast strains genus Saccharomyces. One five-base (ScrFI) two four-base cutting (HaeIII, MspI) restriction enzymes used. The primers 21 M13 core sequence selected RAPD analysis. PCR-RFLP rDNA with HaeIII, ScrFI MspI differentiated tested into four, five four types patterns, respectively...