Glycerol is a by-product generated in large amounts during the production of biofuels. This study presents an alternative means crude glycerol valorization through erythritol and mannitol. In shake-flasks experiment buffered medium, nine Yarrowia lipolytica strains were examined for polyols production. Three (A UV'1, A-15 Wratislavia K1) selected as promising producers or/and mannitol used bioreactor batch cultures fed-batch mode. Pure biodiesel-derived media both supplemented (to 2.5 3.25...

Avian pathogenic Escherichia coli (APEC) is a principal etiologic agent of avian colibacillosis, responsible for significant economic losses in the poultry industry due to high mortality and disease treatment with antibiotics. APEC its ability form biofilms on food processing surfaces contributes persistence within farms. Bacteriophages are promising antibacterial agents combating APEC. This study focused characterization newly isolated phages UPWr_E1, UPWr_E2, UPWr_E4 as well UPWr_E124 phage...

Citric acid and erythritol biosynthesis from pure crude glycerol by three acetate-negative mutants of Yarrowia lipolytica yeast was investigated in batch cultures a wide pH range (3.0–6.5). the most effective at 5.0–5.5 case Wratislavia 1.31 AWG7. With decreasing value, direction changed into synthesis accompanied low production citric acid. Pathways conversion were K1 cells. Enzymatic activity compared run 3.0 4.5, that is, under conditions promoting acid, respectively. The effect value...

Erythritol is a polyol with sweet taste but low energy value. Thanks to its valuable properties, as well growing social awareness and nutritional trends, popularity rapidly. The aim of this study was increase the effectiveness erythritol production from glucose using new UV mutants yeast Yarrowia lipolytica obtained in Wratislavia K1 strain. ability strains biosynthesize utilize examined shake-flask cultures fed-batch processes conducted stirred tank reactor total concentration 300 400 g/L....

This study investigates the waste-free bioconversion of glucose to erythritol using UV-mutagenized strain Yarrowia lipolytica Wratislavia KI UV15. research focuses on optimizing fermentation parameters enhance yield, with a key emphasis utilizing post-crystallization filtrate as primary carbon source, promoting cost-effective and sustainable approach production. The experimental design included systematic variations in ammonium sulfate concentration, yeast extract supplementation, initial...

The aim of this study was to examine the impact divalent copper, iron, manganese, and zinc ions on production erythritol from glycerol by Yarrowia lipolytica their effect activity erythrose reductase. No inhibitory examined minerals yeast growth observed in study. Supplementation with MnSO4 · 7H2O (25 mg l(-1)) increased Y. 14.5%. In bioreactor culture manganese ion addition, 47.1 g l(-1) produced 100.0 glycerol, which corresponded volumetric productivity 0.87 h(-1). addition Mn(2+) enhanced...

Citric acid and erythritol are obtained on an industrial scale using biotechnological methods. Due to the growing market demand for these products, research is underway improve process economics by introducing new microorganisms, in particular of species Yarrowia lipolytica. The aim this study was evaluate transformants Y. lipolytica growth ability overproduce citric acids from glycerol. were constructed overexpressing glycerol kinase, methylcitrate synthase mitochondrial succinate-fumarate...

Erythritol is a natural compound of great interest in food production because its very low caloric value, lack off-taste and side-effects from the gastric system even when consumed with in excess. In present study, the effect agitation rates 500 to 1100 rev/min on biomass erythritol glycerol media by an acetate-negative mutant of Yarrowia lipolytica Wratislavia K1 batch culture was studied. At constant aeration rate 0.36 vvm, between 900 rev/min wasfound suitable for efficient production. In...

This study demonstrates the potential applicability of UV mutant Yarrowia lipolytica MK1 for valorisation glycerol and erythritol production in a chemostat culture. The aim this research is to investigate optimal C:N ratio feeding medium order enhance production. highest concentration, at 113.1 g/L with volumetric rate 1.1 g/(L·h) yield 0.57 g/g, was obtained 80:1. Moreover, no residual observed culture broth during cultivation. chemical composition biomass analysed. contents lysine...

The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and natural method culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources of nitrogen other nutrients without compromising activities enzymes responsible its level. As consequence, process was performed where Y. A-6 able to produce 108.0 g/L erythritol,...

The microbiological biosynthesis of α-ketoglutaric acid (KGA) has recently captured the attention many scientists as an alternative to its common chemical synthesis. present study aimed evaluate effect feeding strategy substrates, i.e., glycerol (G = 20 g·dm−3) and rapeseed oil (O g·dm−3), on yeast growth parameters KGA by a wild strain Yarrowia lipolytica A-8 in fed-batch repeated-batch cultures. effectiveness was demonstrated depend thiamine concentration substrate method. In culture...

Yarrowia lipolytica, which is model oleaginous yeast with high industrial interest, was cultivated on fatty substrates. Data concerning acid composition of both substrate and lipids comparisons the experimental data predictions presented in "Biomodification fats oils scenarios adding value renewable materials through microbial fermentations: Modelling trials lipolytica" (Vasiliadou et al., 2018) were provided. Furthermore, total fractionated into their main fractions, that is, phospholipids,...

The present study aimed to develop a technology for the production of dietary supplements based on yeast biomass and α-ketoglutaric acid (KGA), produced by new transformant Yarrowia lipolytica with improved KGA biosynthesis ability, as well verify usefulness obtained products food feed purposes. Transformants Y. were constructed overexpress genes encoding glycerol kinase, methylcitrate synthase mitochondrial organic transporter. strains compared in terms growth ability glycerol- oil-based...

Most of the world's annual production mannitol is by chemical means, but, due to increasing demand for natural sweeteners, alternative methods are being sought. The aim study was screen Yarrowia lipolytica yeast strains and select culture conditions efficient selective biosynthesis from glycerol. From 21 examined in shake-flask glycerol (100 g/L), three were selected-S2, S3, S4-and further evaluated batch bioreactor cultures with technical raw (150 g/L). best parameters observed strain which...

With the problems related to chemical methods of pyruvic acid (PA) synthesis, a fast-growing interest has been observed in research aiming at reducing production cost PA by applying biotechnological methods. This study aimed investigate potential applicability Yarrowia lipolytica Wratislavia 1.31 yeast strain for valorisation pure and crude glycerol through industrially desired PA. Conditions required effective biosynthesis, i.e., pH value, thiamine concentration, agitation, substrate were...

α-Ketoglutaric acid (KGA) is a valuable compound with wide range of applications, e.g., in the cosmetics, pharmaceutical, chemical and food industries. The present study aimed to enhance efficiency KGA production by Yarrowia lipolytica CBS146773 from renewable carbon sources. In investigation, various factors that may potentially affect biosynthesis were examined bioreactor cultures performed on simple medium containing glycerol (20 g/L) fed four portions substrate mixture (15 + 15 g/L...